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Immobilized enzyme electrode for creatinine determination in serum.
Anal Chem. 1991 Mar 15; 63(6):611-4.AC

Abstract

An immobilized enzyme electrode for continuous creatinine determination in blood serum is described. The enzymes creatinine amidohydrolase, creatine amidinohydrolase, and sarcosine oxidase are coimmobilized to the surface of the polypropylene membrane of a Clark-type electrode responsive to oxygen. The immobilized enzymes catalyze the decomposition of creatinine with the consumption of oxygen and thus permit the creatinine measurement. The whole assay takes less than 1 min. Effects of pH and temperature on electrode response are also described. The proposed technique offers a rapid, simple, and inexpensive means to determine creatinine in blood serum within the normal and abnormal ranges. The repeatability of the creatine determination in serum is 2.5% (relative standard deviation), and the detection limit is 3 x 10(-6) mol L-1. The results obtained by this method were compared to those obtained with the Technicon AutoAnalyzer SMAC system based on the Jaffé reaction; the correlation factor between the two methods was found to be r = 0.9997.

Authors+Show Affiliations

Nguyen VK
Laboratoire de Chimie Physique et Electrochimie, Unité associée au CNRS No. 405, Ecole des Hautes Etudes des Industries Chimiques de Strasbourg, France.
Wolff CM
No affiliation info available
Seris JL
No affiliation info available
Schwing JP
No affiliation info available

MeSH

AmidohydrolasesCreatinineElectrodesEnzymes, ImmobilizedHumansHydrogen-Ion ConcentrationOxidoreductases, N-DemethylatingSarcosine OxidaseTemperatureUreohydrolases

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2031562

Citation

Nguyen, V K., et al. "Immobilized Enzyme Electrode for Creatinine Determination in Serum." Analytical Chemistry, vol. 63, no. 6, 1991, pp. 611-4.
Nguyen VK, Wolff CM, Seris JL, et al. Immobilized enzyme electrode for creatinine determination in serum. Anal Chem. 1991;63(6):611-4.
Nguyen, V. K., Wolff, C. M., Seris, J. L., & Schwing, J. P. (1991). Immobilized enzyme electrode for creatinine determination in serum. Analytical Chemistry, 63(6), 611-4.
Nguyen VK, et al. Immobilized Enzyme Electrode for Creatinine Determination in Serum. Anal Chem. 1991 Mar 15;63(6):611-4. PubMed PMID: 2031562.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immobilized enzyme electrode for creatinine determination in serum. AU - Nguyen,V K, AU - Wolff,C M, AU - Seris,J L, AU - Schwing,J P, PY - 1991/3/15/pubmed PY - 1991/3/15/medline PY - 1991/3/15/entrez SP - 611 EP - 4 JF - Analytical chemistry JO - Anal Chem VL - 63 IS - 6 N2 - An immobilized enzyme electrode for continuous creatinine determination in blood serum is described. The enzymes creatinine amidohydrolase, creatine amidinohydrolase, and sarcosine oxidase are coimmobilized to the surface of the polypropylene membrane of a Clark-type electrode responsive to oxygen. The immobilized enzymes catalyze the decomposition of creatinine with the consumption of oxygen and thus permit the creatinine measurement. The whole assay takes less than 1 min. Effects of pH and temperature on electrode response are also described. The proposed technique offers a rapid, simple, and inexpensive means to determine creatinine in blood serum within the normal and abnormal ranges. The repeatability of the creatine determination in serum is 2.5% (relative standard deviation), and the detection limit is 3 x 10(-6) mol L-1. The results obtained by this method were compared to those obtained with the Technicon AutoAnalyzer SMAC system based on the Jaffé reaction; the correlation factor between the two methods was found to be r = 0.9997. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/2031562/Immobilized_enzyme_electrode_for_creatinine_determination_in_serum_ DB - PRIME DP - Unbound Medicine ER -