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Evaluation of antioxidant defense systems in H4IIE cells infected with a retroviral vector.
Toxicol In Vitro. 2010 Jun; 24(4):1105-10.TV

Abstract

Retroviral gene transfer technology is frequently used to establish stable transgenic cell lines. However, no studies to date have evaluated antioxidant defense systems in cells infected with retroviral particles. In the present study, we examined the effects of retroviral infection on antioxidant defense systems using H4IIE cells infected with a retrovirus that overexpresses green fluorescent protein (retro-H4IIE cells). Total oxyradical scavenging capacity and glutathione (GSH), malondialdehyde, and peroxide levels were not significantly altered in retro-H4IIE cells; however, retro-H4IIE cells showed a higher resistance against cytotoxicity, GSH depletion, and malondialdehyde elevation under H(2)O(2)-induced oxidative stress conditions. Immunoblot analysis showed that alpha-class GSH S-transferase (GST) was increased 2.5-fold in retro-H4IIE cells as compared with H4IIE cells; however, catalase, GSH peroxidase-1, peroxiredoxin-1, and thioredoxin-1 remained unaltered or slightly decreased. l-Buthionine-(S,R)-sulfoximine, a GSH synthesis inhibitor, and 1-chloro-2,4-dinitrobenzene, a GST substrate and competitive inhibitor, decreased the difference in H(2)O(2) responses between the two cell types. These results support the hypothesis that the resistance of retro-H4IIE cells to H(2)O(2) can be attributed to an increase in alpha-class GST expression, as levels of GSH and GSH peroxidase-1 were not altered. The present study suggests that antioxidant enzyme expression may change during the establishment of stable transformed cell lines using retroviral techniques.

Authors+Show Affiliations

College of Pharmacy and RCTCP, Chungnam National University, 220 Gung-dong, Yuseong-gu, Daejeon 305-764, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20332021

Citation

Oh, Soo Jin, et al. "Evaluation of Antioxidant Defense Systems in H4IIE Cells Infected With a Retroviral Vector." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 24, no. 4, 2010, pp. 1105-10.
Oh SJ, Chae J, Zhu H, et al. Evaluation of antioxidant defense systems in H4IIE cells infected with a retroviral vector. Toxicol In Vitro. 2010;24(4):1105-10.
Oh, S. J., Chae, J., Zhu, H., Hien, T. T., Lee, K., Kim, H. M., Kang, K. W., Song, G. Y., Kang, J. S., Kim, B. H., Kwon, K. I., & Kim, S. K. (2010). Evaluation of antioxidant defense systems in H4IIE cells infected with a retroviral vector. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 24(4), 1105-10. https://doi.org/10.1016/j.tiv.2010.03.010
Oh SJ, et al. Evaluation of Antioxidant Defense Systems in H4IIE Cells Infected With a Retroviral Vector. Toxicol In Vitro. 2010;24(4):1105-10. PubMed PMID: 20332021.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of antioxidant defense systems in H4IIE cells infected with a retroviral vector. AU - Oh,Soo Jin, AU - Chae,Jooyoung, AU - Zhu,Hongmei, AU - Hien,Tran Thi, AU - Lee,Kiho, AU - Kim,Hwan Mook, AU - Kang,Keon Wook, AU - Song,Gyu Yong, AU - Kang,Jong Seong, AU - Kim,Bong-Hee, AU - Kwon,Kwang-il, AU - Kim,Sang Kyum, Y1 - 2010/03/21/ PY - 2009/10/05/received PY - 2010/02/02/revised PY - 2010/03/17/accepted PY - 2010/3/25/entrez PY - 2010/3/25/pubmed PY - 2010/6/18/medline SP - 1105 EP - 10 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 24 IS - 4 N2 - Retroviral gene transfer technology is frequently used to establish stable transgenic cell lines. However, no studies to date have evaluated antioxidant defense systems in cells infected with retroviral particles. In the present study, we examined the effects of retroviral infection on antioxidant defense systems using H4IIE cells infected with a retrovirus that overexpresses green fluorescent protein (retro-H4IIE cells). Total oxyradical scavenging capacity and glutathione (GSH), malondialdehyde, and peroxide levels were not significantly altered in retro-H4IIE cells; however, retro-H4IIE cells showed a higher resistance against cytotoxicity, GSH depletion, and malondialdehyde elevation under H(2)O(2)-induced oxidative stress conditions. Immunoblot analysis showed that alpha-class GSH S-transferase (GST) was increased 2.5-fold in retro-H4IIE cells as compared with H4IIE cells; however, catalase, GSH peroxidase-1, peroxiredoxin-1, and thioredoxin-1 remained unaltered or slightly decreased. l-Buthionine-(S,R)-sulfoximine, a GSH synthesis inhibitor, and 1-chloro-2,4-dinitrobenzene, a GST substrate and competitive inhibitor, decreased the difference in H(2)O(2) responses between the two cell types. These results support the hypothesis that the resistance of retro-H4IIE cells to H(2)O(2) can be attributed to an increase in alpha-class GST expression, as levels of GSH and GSH peroxidase-1 were not altered. The present study suggests that antioxidant enzyme expression may change during the establishment of stable transformed cell lines using retroviral techniques. SN - 1879-3177 UR - https://www.unboundmedicine.com/medline/citation/20332021/Evaluation_of_antioxidant_defense_systems_in_H4IIE_cells_infected_with_a_retroviral_vector_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(10)00074-3 DB - PRIME DP - Unbound Medicine ER -