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Regulatory effects of insulin-like growth factor-1 on the expression of sensory neuropeptide mRNAs in cultured dorsal root ganglion neurons with excitotoxicity induced by glutamate.
Neurosci Bull. 2010 Apr; 26(2):126-32.NB

Abstract

OBJECTIVE

To determine the effects of insulin-like growth factor-1 (IGF-1) on the expression of preprotachykinin (PPT) mRNA encoding substance P (SP) and calcitonin gene-related peptide (CGRP) mRNA in cultured dorsal root ganglion (DRG) neurons with excitotoxicity induced by glutamate (Glu).

METHODS

DRGs were dissected from embryonic day 15 Wistar rats. DRG neurons were dissociated and cultured for 48 h and then exposed to Glu (0.2 mmol/L) or Glu (0.2 mmol/L) plus IGF-1 (5 nmol/L, 10 nmol/L and 20 nmol/L) for 12 h. The DRG neurons in control group were exposed to only growth media throughout the experiment. After that, the living DRG neurons were observed under inverted phase contrast microscope and microphotographs were taken. The expression levels of PPT and CGRP mRNAs were detected by reverse transcription-polymerase chain reaction (RT-PCR).

RESULTS

IGF-1 could inhibit Glu-induced shortening of neurite. Besides, IGF-1 could significantly increase the levels of PPT mRNA and CGRP mRNA in primary cultured DRG neurons with Glu-induced excitotoxicity, in a dose-dependent manner.

CONCLUSION

IGF-1 may exert neuroprotective effects on DRG neurons against Glu-induced excitotoxicity, probably through regulating the expression levels of PPT and CGRP mRNAs.

Authors+Show Affiliations

Department of Anatomy, Shandong University School of Medicine, Jinan 250012, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20332817

Citation

Gong, Hui-Cui, et al. "Regulatory Effects of Insulin-like Growth Factor-1 On the Expression of Sensory Neuropeptide mRNAs in Cultured Dorsal Root Ganglion Neurons With Excitotoxicity Induced By Glutamate." Neuroscience Bulletin, vol. 26, no. 2, 2010, pp. 126-32.
Gong HC, Yang XD, Liu Z, et al. Regulatory effects of insulin-like growth factor-1 on the expression of sensory neuropeptide mRNAs in cultured dorsal root ganglion neurons with excitotoxicity induced by glutamate. Neurosci Bull. 2010;26(2):126-32.
Gong, H. C., Yang, X. D., Liu, Z., Xing, Z. Y., Wang, H. J., & Li, Z. Z. (2010). Regulatory effects of insulin-like growth factor-1 on the expression of sensory neuropeptide mRNAs in cultured dorsal root ganglion neurons with excitotoxicity induced by glutamate. Neuroscience Bulletin, 26(2), 126-32. https://doi.org/10.1007/s12264-010-9142-0
Gong HC, et al. Regulatory Effects of Insulin-like Growth Factor-1 On the Expression of Sensory Neuropeptide mRNAs in Cultured Dorsal Root Ganglion Neurons With Excitotoxicity Induced By Glutamate. Neurosci Bull. 2010;26(2):126-32. PubMed PMID: 20332817.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulatory effects of insulin-like growth factor-1 on the expression of sensory neuropeptide mRNAs in cultured dorsal root ganglion neurons with excitotoxicity induced by glutamate. AU - Gong,Hui-Cui, AU - Yang,Xiang-Dong, AU - Liu,Zhen, AU - Xing,Zi-Ying, AU - Wang,Huai-Jing, AU - Li,Zhen-Zhong, PY - 2010/3/25/entrez PY - 2010/3/25/pubmed PY - 2010/7/8/medline SP - 126 EP - 32 JF - Neuroscience bulletin JO - Neurosci Bull VL - 26 IS - 2 N2 - OBJECTIVE: To determine the effects of insulin-like growth factor-1 (IGF-1) on the expression of preprotachykinin (PPT) mRNA encoding substance P (SP) and calcitonin gene-related peptide (CGRP) mRNA in cultured dorsal root ganglion (DRG) neurons with excitotoxicity induced by glutamate (Glu). METHODS: DRGs were dissected from embryonic day 15 Wistar rats. DRG neurons were dissociated and cultured for 48 h and then exposed to Glu (0.2 mmol/L) or Glu (0.2 mmol/L) plus IGF-1 (5 nmol/L, 10 nmol/L and 20 nmol/L) for 12 h. The DRG neurons in control group were exposed to only growth media throughout the experiment. After that, the living DRG neurons were observed under inverted phase contrast microscope and microphotographs were taken. The expression levels of PPT and CGRP mRNAs were detected by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: IGF-1 could inhibit Glu-induced shortening of neurite. Besides, IGF-1 could significantly increase the levels of PPT mRNA and CGRP mRNA in primary cultured DRG neurons with Glu-induced excitotoxicity, in a dose-dependent manner. CONCLUSION: IGF-1 may exert neuroprotective effects on DRG neurons against Glu-induced excitotoxicity, probably through regulating the expression levels of PPT and CGRP mRNAs. SN - 1995-8218 UR - https://www.unboundmedicine.com/medline/citation/20332817/Regulatory_effects_of_insulin_like_growth_factor_1_on_the_expression_of_sensory_neuropeptide_mRNAs_in_cultured_dorsal_root_ganglion_neurons_with_excitotoxicity_induced_by_glutamate_ L2 - https://dx.doi.org/10.1007/s12264-010-9142-0 DB - PRIME DP - Unbound Medicine ER -