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Antioxidant capacity, phenolic content, and profiling of phenolic compounds in the seeds, skin, and pulp of Vitis rotundifolia (Muscadine Grapes) As determined by HPLC-DAD-ESI-MS(n).
J Agric Food Chem. 2010 Apr 28; 58(8):4681-92.JA

Abstract

The objective of this study was to determine and compare the total phenolic content and antioxidant capacity in the seeds, skin, and pulp of eight cultivars of Florida-grown muscadine grapes and to identify the phenolic compounds in these respective portions. Total phenolic contents were determined colorimetrically using Folin-Ciocalteu reagent, and antioxidant capacity was determined by oxygen radical absorbance capacity (ORAC). High-performance liquid chromatography equipped with diode array (HPLC-DAD) and electrospray ionization mass spectrometric detection (ESI-MS(n)) was used to identify the phenolic compounds in the seeds, skin, and pulp of muscadine grapes. The total phenolic content and antioxidant capacity, based on fresh weight, were highest in seeds followed by skin and pulp. On average, 87.1, 11.3, and 1.6% of phenolic compounds were present in seeds, skin, and pulp, respectively. A total of 88 phenolic compounds of diverse structures were tentatively identified in muscadines, which included 17 in the pulp, 28 in the skin, and 43 in the seeds. Seventeen compounds were identified for the first time in muscadine grapes. The compounds identified in seeds included hydrolyzable tannins, flavan-3-ols and condensed tannins, ellagic acid derivatives, and quercetin rhamnoside. The skin contained hydrolyzable tannins, flavonoids, including anthocyanin 3,5-diglucosides, quercetin, myricetin, and kaempferol glycosides.

Authors+Show Affiliations

Department of Food Science and Human Nutrition, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, Florida 32611, USA.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20334341

Citation

Sandhu, Amandeep K., and Liwei Gu. "Antioxidant Capacity, Phenolic Content, and Profiling of Phenolic Compounds in the Seeds, Skin, and Pulp of Vitis Rotundifolia (Muscadine Grapes) as Determined By HPLC-DAD-ESI-MS(n)." Journal of Agricultural and Food Chemistry, vol. 58, no. 8, 2010, pp. 4681-92.
Sandhu AK, Gu L. Antioxidant capacity, phenolic content, and profiling of phenolic compounds in the seeds, skin, and pulp of Vitis rotundifolia (Muscadine Grapes) As determined by HPLC-DAD-ESI-MS(n). J Agric Food Chem. 2010;58(8):4681-92.
Sandhu, A. K., & Gu, L. (2010). Antioxidant capacity, phenolic content, and profiling of phenolic compounds in the seeds, skin, and pulp of Vitis rotundifolia (Muscadine Grapes) As determined by HPLC-DAD-ESI-MS(n). Journal of Agricultural and Food Chemistry, 58(8), 4681-92. https://doi.org/10.1021/jf904211q
Sandhu AK, Gu L. Antioxidant Capacity, Phenolic Content, and Profiling of Phenolic Compounds in the Seeds, Skin, and Pulp of Vitis Rotundifolia (Muscadine Grapes) as Determined By HPLC-DAD-ESI-MS(n). J Agric Food Chem. 2010 Apr 28;58(8):4681-92. PubMed PMID: 20334341.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant capacity, phenolic content, and profiling of phenolic compounds in the seeds, skin, and pulp of Vitis rotundifolia (Muscadine Grapes) As determined by HPLC-DAD-ESI-MS(n). AU - Sandhu,Amandeep K, AU - Gu,Liwei, PY - 2010/3/26/entrez PY - 2010/3/26/pubmed PY - 2010/7/27/medline SP - 4681 EP - 92 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 58 IS - 8 N2 - The objective of this study was to determine and compare the total phenolic content and antioxidant capacity in the seeds, skin, and pulp of eight cultivars of Florida-grown muscadine grapes and to identify the phenolic compounds in these respective portions. Total phenolic contents were determined colorimetrically using Folin-Ciocalteu reagent, and antioxidant capacity was determined by oxygen radical absorbance capacity (ORAC). High-performance liquid chromatography equipped with diode array (HPLC-DAD) and electrospray ionization mass spectrometric detection (ESI-MS(n)) was used to identify the phenolic compounds in the seeds, skin, and pulp of muscadine grapes. The total phenolic content and antioxidant capacity, based on fresh weight, were highest in seeds followed by skin and pulp. On average, 87.1, 11.3, and 1.6% of phenolic compounds were present in seeds, skin, and pulp, respectively. A total of 88 phenolic compounds of diverse structures were tentatively identified in muscadines, which included 17 in the pulp, 28 in the skin, and 43 in the seeds. Seventeen compounds were identified for the first time in muscadine grapes. The compounds identified in seeds included hydrolyzable tannins, flavan-3-ols and condensed tannins, ellagic acid derivatives, and quercetin rhamnoside. The skin contained hydrolyzable tannins, flavonoids, including anthocyanin 3,5-diglucosides, quercetin, myricetin, and kaempferol glycosides. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/20334341/Antioxidant_capacity_phenolic_content_and_profiling_of_phenolic_compounds_in_the_seeds_skin_and_pulp_of_Vitis_rotundifolia__Muscadine_Grapes__As_determined_by_HPLC_DAD_ESI_MS_n__ L2 - https://doi.org/10.1021/jf904211q DB - PRIME DP - Unbound Medicine ER -