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Repeatability of the measurement of exhaled volatile metabolites using selected ion flow tube mass spectrometry.
J Am Soc Mass Spectrom. 2010 Jun; 21(6):1070-4.JA

Abstract

Selected ion flow tube mass spectrometry, SIFT-MS, has been used to determine the repeatability of the analysis of volatile metabolites within the breath of healthy volunteers, with emphasis on the influence of sampling methodology. Baseline instrument specific coefficients of variability for examined metabolites were as follows: acetone (1%), ammonia (1%), isoprene (2%), propanol (6%), ethanol (7%), acetic acid (7%), and hydrogen cyanide (19%). Metabolite concentration and related product ion count rate were identified as strong determinants of measurement variation. With the exception of ammonia, an orally released metabolite, variability in repeated on-line breath analysis tended to be lower for metabolites of systemic origin. Standardization of sampling technique improved the repeatability of the analysis of selected metabolites. Off-line (bag) alveolar breath sampling, as opposed to mixed (whole) breath sampling, likewise improved the repeatability of the analysis of all metabolites investigated, with the exception of acetic acid. We conclude that SIFT-MS analysis of common volatile metabolites within the breath of healthy volunteers is both reliable and repeatable. For selected metabolites, the finding that repeatability is improved through modification of sampling methodology may have implications in terms of future recommended practices.

Authors+Show Affiliations

Department of Biosurgery and Surgical Technology, Imperial College London, St. Mary's Hospital, London, United Kingdom.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20335048

Citation

Boshier, Piers R., et al. "Repeatability of the Measurement of Exhaled Volatile Metabolites Using Selected Ion Flow Tube Mass Spectrometry." Journal of the American Society for Mass Spectrometry, vol. 21, no. 6, 2010, pp. 1070-4.
Boshier PR, Marczin N, Hanna GB. Repeatability of the measurement of exhaled volatile metabolites using selected ion flow tube mass spectrometry. J Am Soc Mass Spectrom. 2010;21(6):1070-4.
Boshier, P. R., Marczin, N., & Hanna, G. B. (2010). Repeatability of the measurement of exhaled volatile metabolites using selected ion flow tube mass spectrometry. Journal of the American Society for Mass Spectrometry, 21(6), 1070-4. https://doi.org/10.1016/j.jasms.2010.02.008
Boshier PR, Marczin N, Hanna GB. Repeatability of the Measurement of Exhaled Volatile Metabolites Using Selected Ion Flow Tube Mass Spectrometry. J Am Soc Mass Spectrom. 2010;21(6):1070-4. PubMed PMID: 20335048.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Repeatability of the measurement of exhaled volatile metabolites using selected ion flow tube mass spectrometry. AU - Boshier,Piers R, AU - Marczin,Nandor, AU - Hanna,George B, Y1 - 2010/02/10/ PY - 2009/11/17/received PY - 2010/02/05/revised PY - 2010/02/06/accepted PY - 2010/3/26/entrez PY - 2010/3/26/pubmed PY - 2010/9/11/medline SP - 1070 EP - 4 JF - Journal of the American Society for Mass Spectrometry JO - J Am Soc Mass Spectrom VL - 21 IS - 6 N2 - Selected ion flow tube mass spectrometry, SIFT-MS, has been used to determine the repeatability of the analysis of volatile metabolites within the breath of healthy volunteers, with emphasis on the influence of sampling methodology. Baseline instrument specific coefficients of variability for examined metabolites were as follows: acetone (1%), ammonia (1%), isoprene (2%), propanol (6%), ethanol (7%), acetic acid (7%), and hydrogen cyanide (19%). Metabolite concentration and related product ion count rate were identified as strong determinants of measurement variation. With the exception of ammonia, an orally released metabolite, variability in repeated on-line breath analysis tended to be lower for metabolites of systemic origin. Standardization of sampling technique improved the repeatability of the analysis of selected metabolites. Off-line (bag) alveolar breath sampling, as opposed to mixed (whole) breath sampling, likewise improved the repeatability of the analysis of all metabolites investigated, with the exception of acetic acid. We conclude that SIFT-MS analysis of common volatile metabolites within the breath of healthy volunteers is both reliable and repeatable. For selected metabolites, the finding that repeatability is improved through modification of sampling methodology may have implications in terms of future recommended practices. SN - 1879-1123 UR - https://www.unboundmedicine.com/medline/citation/20335048/Repeatability_of_the_measurement_of_exhaled_volatile_metabolites_using_selected_ion_flow_tube_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1044-0305(10)00102-9 DB - PRIME DP - Unbound Medicine ER -