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Up-regulation of crystallins is involved in the neuroprotective effect of wolfberry on survival of retinal ganglion cells in rat ocular hypertension model.
J Cell Biochem. 2010 May 15; 110(2):311-20.JC

Abstract

Wolfberry (fruit of Lycium barbarum Linn) has been known for balancing 'Yin' and 'Yang' in the body, nourishing the liver and kidney, improving visual acuity for more than 2,500 years in oriental countries. The active components in wolfberry include L. barbarum polysaccharide (LBP), zeaxanthine, betaine, cerebroside and trace amounts of zinc, iron, and copper. Each of them confers distinct beneficial effects and together they help to explain widespread use of wolfberry in the eastern world. Earlier study reported the neuroprotective effects of LBP on retinal ganglion cell (RGC) in an experimental model of glaucoma and the underlying in vivo cellular mechanisms of LBP neuroprotection deserve further exploration. In this study, we adopted proteomics, functional genomics, to evaluate pharmacological effects of LBP on the neuronal survival pathways. Among the significantly changed proteins induced by LBP feeding on ocular hypertension (OH) retinas, only proteins in crystallin family were focused in this study. The proteomic results were further confirmed using the Western blotting of the retinas and immunohistochemical staining of the retinal sections. We demonstrated that neuroprotective effect of-wolfberry extract-LBP on the survival of RGCs may be mediated via direct up-regulation of neuronal survival signal betaB2-crystallin.

Authors+Show Affiliations

Laboratory of Neurodegenerative Diseases, Department of Anatomy, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20336662

Citation

Chiu, Kin, et al. "Up-regulation of Crystallins Is Involved in the Neuroprotective Effect of Wolfberry On Survival of Retinal Ganglion Cells in Rat Ocular Hypertension Model." Journal of Cellular Biochemistry, vol. 110, no. 2, 2010, pp. 311-20.
Chiu K, Zhou Y, Yeung SC, et al. Up-regulation of crystallins is involved in the neuroprotective effect of wolfberry on survival of retinal ganglion cells in rat ocular hypertension model. J Cell Biochem. 2010;110(2):311-20.
Chiu, K., Zhou, Y., Yeung, S. C., Lok, C. K., Chan, O. O., Chang, R. C., So, K. F., & Chiu, J. F. (2010). Up-regulation of crystallins is involved in the neuroprotective effect of wolfberry on survival of retinal ganglion cells in rat ocular hypertension model. Journal of Cellular Biochemistry, 110(2), 311-20. https://doi.org/10.1002/jcb.22539
Chiu K, et al. Up-regulation of Crystallins Is Involved in the Neuroprotective Effect of Wolfberry On Survival of Retinal Ganglion Cells in Rat Ocular Hypertension Model. J Cell Biochem. 2010 May 15;110(2):311-20. PubMed PMID: 20336662.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Up-regulation of crystallins is involved in the neuroprotective effect of wolfberry on survival of retinal ganglion cells in rat ocular hypertension model. AU - Chiu,Kin, AU - Zhou,Yuan, AU - Yeung,Sze-Chun, AU - Lok,Carmen Ka-Ming, AU - Chan,Owen O-Cheung, AU - Chang,Raymond Chuen-Chung, AU - So,Kwok-Fai, AU - Chiu,Jin-Fu, PY - 2010/3/26/entrez PY - 2010/3/26/pubmed PY - 2010/8/28/medline SP - 311 EP - 20 JF - Journal of cellular biochemistry JO - J Cell Biochem VL - 110 IS - 2 N2 - Wolfberry (fruit of Lycium barbarum Linn) has been known for balancing 'Yin' and 'Yang' in the body, nourishing the liver and kidney, improving visual acuity for more than 2,500 years in oriental countries. The active components in wolfberry include L. barbarum polysaccharide (LBP), zeaxanthine, betaine, cerebroside and trace amounts of zinc, iron, and copper. Each of them confers distinct beneficial effects and together they help to explain widespread use of wolfberry in the eastern world. Earlier study reported the neuroprotective effects of LBP on retinal ganglion cell (RGC) in an experimental model of glaucoma and the underlying in vivo cellular mechanisms of LBP neuroprotection deserve further exploration. In this study, we adopted proteomics, functional genomics, to evaluate pharmacological effects of LBP on the neuronal survival pathways. Among the significantly changed proteins induced by LBP feeding on ocular hypertension (OH) retinas, only proteins in crystallin family were focused in this study. The proteomic results were further confirmed using the Western blotting of the retinas and immunohistochemical staining of the retinal sections. We demonstrated that neuroprotective effect of-wolfberry extract-LBP on the survival of RGCs may be mediated via direct up-regulation of neuronal survival signal betaB2-crystallin. SN - 1097-4644 UR - https://www.unboundmedicine.com/medline/citation/20336662/Up_regulation_of_crystallins_is_involved_in_the_neuroprotective_effect_of_wolfberry_on_survival_of_retinal_ganglion_cells_in_rat_ocular_hypertension_model_ L2 - https://doi.org/10.1002/jcb.22539 DB - PRIME DP - Unbound Medicine ER -