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Norovirus GII-4 2006b variant circulating in patients with acute gastroenteritis in Thailand during a 2006-2007 study.
J Med Virol. 2010 May; 82(5):854-60.JM

Abstract

Noroviruses (NoVs) are recognized as a significant cause of acute gastroenteritis in children and adults. A 14-month study, from January 2006 to February 2007, was undertaken in a hospital in Thailand to determine the prevalence and genetic characterization of NoVs in patients of all ages with acute gastroenteritis. Based on reverse transcription-nested polymerase chain reaction (RT-nested PCR), NoVs were detected in 122 of 273 (44.7%) collected stool samples. Of the 122 NoV-positive samples, 28 (23%) belonged to GI, 79 (64.8%) belonged to GII, and 15 (12.2%) were mixed infections of GI and GII strains. Three NoV GI-positive and 42 NoV GII-positive samples were characterized successfully by DNA sequencing of the RT-nested PCR products and phylogenetic analysis. For NoV GI, two genotypes were identified: GI-2 (one sample) and GI-6 (two samples). NoV GII could be classified further into five distinct genotypes: GII-2 (1 sample), GII-3 (3 samples), GII-4 (14 samples), GII-6 (3 samples), and GII-17 (2 samples), and one unclassified genotype (19 samples). All NoV GII-4 strains showed 88-98% nucleotide identity with NoV GII-4 2006b variants reported worldwide. Among genotypes of NoV characterized, one co-infected stool sample exhibited NoVs GI-6 and GII-4 2006b. This study suggests that there is an important role of NoVs as etiologic agents in patients with acute gastroenteritis. The predominant circulating genotype of NoV infections is GII-4 2006b variant.

Authors+Show Affiliations

Department of Microbiology, Faculty of Public Health, Mahidol University, Bangkok, Thailand. phlkt@mahidol.ac.thNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20336729

Citation

Kittigul, Leera, et al. "Norovirus GII-4 2006b Variant Circulating in Patients With Acute Gastroenteritis in Thailand During a 2006-2007 Study." Journal of Medical Virology, vol. 82, no. 5, 2010, pp. 854-60.
Kittigul L, Pombubpa K, Taweekate Y, et al. Norovirus GII-4 2006b variant circulating in patients with acute gastroenteritis in Thailand during a 2006-2007 study. J Med Virol. 2010;82(5):854-60.
Kittigul, L., Pombubpa, K., Taweekate, Y., Diraphat, P., Sujirarat, D., Khamrin, P., & Ushijima, H. (2010). Norovirus GII-4 2006b variant circulating in patients with acute gastroenteritis in Thailand during a 2006-2007 study. Journal of Medical Virology, 82(5), 854-60. https://doi.org/10.1002/jmv.21746
Kittigul L, et al. Norovirus GII-4 2006b Variant Circulating in Patients With Acute Gastroenteritis in Thailand During a 2006-2007 Study. J Med Virol. 2010;82(5):854-60. PubMed PMID: 20336729.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Norovirus GII-4 2006b variant circulating in patients with acute gastroenteritis in Thailand during a 2006-2007 study. AU - Kittigul,Leera, AU - Pombubpa,Kannika, AU - Taweekate,Yuthana, AU - Diraphat,Pornphan, AU - Sujirarat,Dusit, AU - Khamrin,Pattara, AU - Ushijima,Hiroshi, PY - 2010/3/26/entrez PY - 2010/3/26/pubmed PY - 2010/6/16/medline SP - 854 EP - 60 JF - Journal of medical virology JO - J Med Virol VL - 82 IS - 5 N2 - Noroviruses (NoVs) are recognized as a significant cause of acute gastroenteritis in children and adults. A 14-month study, from January 2006 to February 2007, was undertaken in a hospital in Thailand to determine the prevalence and genetic characterization of NoVs in patients of all ages with acute gastroenteritis. Based on reverse transcription-nested polymerase chain reaction (RT-nested PCR), NoVs were detected in 122 of 273 (44.7%) collected stool samples. Of the 122 NoV-positive samples, 28 (23%) belonged to GI, 79 (64.8%) belonged to GII, and 15 (12.2%) were mixed infections of GI and GII strains. Three NoV GI-positive and 42 NoV GII-positive samples were characterized successfully by DNA sequencing of the RT-nested PCR products and phylogenetic analysis. For NoV GI, two genotypes were identified: GI-2 (one sample) and GI-6 (two samples). NoV GII could be classified further into five distinct genotypes: GII-2 (1 sample), GII-3 (3 samples), GII-4 (14 samples), GII-6 (3 samples), and GII-17 (2 samples), and one unclassified genotype (19 samples). All NoV GII-4 strains showed 88-98% nucleotide identity with NoV GII-4 2006b variants reported worldwide. Among genotypes of NoV characterized, one co-infected stool sample exhibited NoVs GI-6 and GII-4 2006b. This study suggests that there is an important role of NoVs as etiologic agents in patients with acute gastroenteritis. The predominant circulating genotype of NoV infections is GII-4 2006b variant. SN - 1096-9071 UR - https://www.unboundmedicine.com/medline/citation/20336729/Norovirus_GII_4_2006b_variant_circulating_in_patients_with_acute_gastroenteritis_in_Thailand_during_a_2006_2007_study_ L2 - https://doi.org/10.1002/jmv.21746 DB - PRIME DP - Unbound Medicine ER -