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Selenium-enriched milk proteins and selenium yeast affect selenoprotein activity and expression differently in mouse colon.
Br J Nutr 2010; 104(1):17-23BJ

Abstract

Certain forms of dietary Se may have an advantage in improving Se status and reducing cancer risk. The present study compared the effects of an Se-enriched milk protein product (dairy-Se) with an Se yeast (yeast-Se) on selenoprotein activity and expression in the mouse colon. Mice were fed four diets for 4 weeks: a control milk protein diet (Se at 0.068 parts per million (ppm)), dairy-Se diets with Se at 0.5 and 1 ppm, and a yeast-Se diet with Se at 1 ppm. Cytosolic glutathione peroxidase-1 (GPx-1) activity, mRNA of selenoprotein P (SeP), GPx-1, gastrointestinal glutathione peroxidase-2 (GPx-2) and thioredoxin reductase-1 (TrxR-1) were examined in the mouse colon. Dairy-Se diets did not significantly affect GPx-1 mRNA and GPx-1 activity but produced a dose-dependent increase in SeP and GPx-2 mRNA, with a significantly higher level achieved at 1 ppm Se (P < 0.05). Yeast-Se at 1 ppm significantly increased GPx-1 mRNA and GPx-1 activity (P < 0.01) but not GPx-2 mRNA. Neither Se supplement had any effect on TrxR-1. The present study indicates that selenoprotein levels in the mouse colon are regulated differently depending on the Se supplement. As we have previously shown that dairy-Se at 1 ppm was protective against colorectal cancer (CRC) in an azoxymethane-induced CRC mouse model, this up-regulation of colonic GPx-2 and SeP with Se supplementation may be crucial to its chemopreventive action.

Authors+Show Affiliations

Flinders Centre for Cancer Prevention and Control, Flinders University of South Australia, Bedford Park 5042, Australia. ying.hu@flinders.edu.auNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20346189

Citation

Hu, Ying, et al. "Selenium-enriched Milk Proteins and Selenium Yeast Affect Selenoprotein Activity and Expression Differently in Mouse Colon." The British Journal of Nutrition, vol. 104, no. 1, 2010, pp. 17-23.
Hu Y, McIntosh GH, Le Leu RK, et al. Selenium-enriched milk proteins and selenium yeast affect selenoprotein activity and expression differently in mouse colon. Br J Nutr. 2010;104(1):17-23.
Hu, Y., McIntosh, G. H., Le Leu, R. K., & Young, G. P. (2010). Selenium-enriched milk proteins and selenium yeast affect selenoprotein activity and expression differently in mouse colon. The British Journal of Nutrition, 104(1), pp. 17-23. doi:10.1017/S0007114510000309.
Hu Y, et al. Selenium-enriched Milk Proteins and Selenium Yeast Affect Selenoprotein Activity and Expression Differently in Mouse Colon. Br J Nutr. 2010;104(1):17-23. PubMed PMID: 20346189.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selenium-enriched milk proteins and selenium yeast affect selenoprotein activity and expression differently in mouse colon. AU - Hu,Ying, AU - McIntosh,Graeme H, AU - Le Leu,Richard K, AU - Young,Graeme P, Y1 - 2010/03/29/ PY - 2010/3/30/entrez PY - 2010/3/30/pubmed PY - 2010/7/10/medline SP - 17 EP - 23 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 104 IS - 1 N2 - Certain forms of dietary Se may have an advantage in improving Se status and reducing cancer risk. The present study compared the effects of an Se-enriched milk protein product (dairy-Se) with an Se yeast (yeast-Se) on selenoprotein activity and expression in the mouse colon. Mice were fed four diets for 4 weeks: a control milk protein diet (Se at 0.068 parts per million (ppm)), dairy-Se diets with Se at 0.5 and 1 ppm, and a yeast-Se diet with Se at 1 ppm. Cytosolic glutathione peroxidase-1 (GPx-1) activity, mRNA of selenoprotein P (SeP), GPx-1, gastrointestinal glutathione peroxidase-2 (GPx-2) and thioredoxin reductase-1 (TrxR-1) were examined in the mouse colon. Dairy-Se diets did not significantly affect GPx-1 mRNA and GPx-1 activity but produced a dose-dependent increase in SeP and GPx-2 mRNA, with a significantly higher level achieved at 1 ppm Se (P < 0.05). Yeast-Se at 1 ppm significantly increased GPx-1 mRNA and GPx-1 activity (P < 0.01) but not GPx-2 mRNA. Neither Se supplement had any effect on TrxR-1. The present study indicates that selenoprotein levels in the mouse colon are regulated differently depending on the Se supplement. As we have previously shown that dairy-Se at 1 ppm was protective against colorectal cancer (CRC) in an azoxymethane-induced CRC mouse model, this up-regulation of colonic GPx-2 and SeP with Se supplementation may be crucial to its chemopreventive action. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/20346189/Selenium_enriched_milk_proteins_and_selenium_yeast_affect_selenoprotein_activity_and_expression_differently_in_mouse_colon_ L2 - https://www.cambridge.org/core/product/identifier/S0007114510000309/type/journal_article DB - PRIME DP - Unbound Medicine ER -