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Association between Faecalibacterium prausnitzii and dietary fibre in colonic fermentation in healthy human subjects.
Br J Nutr. 2010 Sep; 104(5):693-700.BJ

Abstract

The intestinal microbiota are a complex ecosystem influencing the immunoregulation of the human host, providing protection from colonising pathogens and producing SCFA as the main energy source of colonocytes. Our objective was to investigate the effect of dietary fibre exclusion and supplementation on the intestinal microbiota and SCFA concentrations. Faecal samples were obtained from healthy volunteers before and after two 14 d periods of consuming formulated diets devoid or supplemented with fibre (14 g/l). The faecal microbiota were analysed using fluorescent in situ hybridisation and SCFA were measured using GLC. There were large and statistically significant reductions in the numbers of the Faecalibacterium prausnitzii (P < or = 0.01) and Roseburia spp. (P < or = 0.01) groups during both the fibre-free and fibre-supplemented diets. Significant and strong positive correlations between the proportion of F. prausnitzii and the proportion of butyrate during both baseline normal diets were found (pre-fibre free r 0.881, P = 0.001; pre-fibre supplemented r 0.844, P = 0.002). A significant correlation was also found between the proportional reduction in F. prausnitzii and the proportional reduction in faecal butyrate during both the fibre-free (r 0.806; P = 0.005) and the fibre-supplemented diet (r 0.749; P = 0.013). These findings may contribute to the understanding of the association between fibre, microbiota and fermentation in health, during enteral nutrition and in disease states such as Crohn's disease.

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Authors+Show Affiliations

Benus RF
Department of Medical Microbiology, University Medical Centre Groningen, University of Groningen, Groningen, The Netherlands. r.f.j.benus@mmb.umcg.nl
van der Werf TS
No affiliation info available
Welling GW
No affiliation info available
Judd PA
No affiliation info available
Taylor MA
No affiliation info available
Harmsen HJ
No affiliation info available
Whelan K
No affiliation info available

MeSH

AdultButyratesColonDietDietary FiberFatty Acids, VolatileFecesFemaleFermentationFusobacteriumHumansMaleYoung Adult

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20346190

Citation

Benus, Robin F J., et al. "Association Between Faecalibacterium Prausnitzii and Dietary Fibre in Colonic Fermentation in Healthy Human Subjects." The British Journal of Nutrition, vol. 104, no. 5, 2010, pp. 693-700.
Benus RF, van der Werf TS, Welling GW, et al. Association between Faecalibacterium prausnitzii and dietary fibre in colonic fermentation in healthy human subjects. Br J Nutr. 2010;104(5):693-700.
Benus, R. F., van der Werf, T. S., Welling, G. W., Judd, P. A., Taylor, M. A., Harmsen, H. J., & Whelan, K. (2010). Association between Faecalibacterium prausnitzii and dietary fibre in colonic fermentation in healthy human subjects. The British Journal of Nutrition, 104(5), 693-700. https://doi.org/10.1017/S0007114510001030
Benus RF, et al. Association Between Faecalibacterium Prausnitzii and Dietary Fibre in Colonic Fermentation in Healthy Human Subjects. Br J Nutr. 2010;104(5):693-700. PubMed PMID: 20346190.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association between Faecalibacterium prausnitzii and dietary fibre in colonic fermentation in healthy human subjects. AU - Benus,Robin F J, AU - van der Werf,Tjip S, AU - Welling,Gjalt W, AU - Judd,Patricia A, AU - Taylor,Moira A, AU - Harmsen,Hermie J M, AU - Whelan,Kevin, Y1 - 2010/03/29/ PY - 2010/3/30/entrez PY - 2010/3/30/pubmed PY - 2010/9/24/medline SP - 693 EP - 700 JF - The British journal of nutrition JO - Br J Nutr VL - 104 IS - 5 N2 - The intestinal microbiota are a complex ecosystem influencing the immunoregulation of the human host, providing protection from colonising pathogens and producing SCFA as the main energy source of colonocytes. Our objective was to investigate the effect of dietary fibre exclusion and supplementation on the intestinal microbiota and SCFA concentrations. Faecal samples were obtained from healthy volunteers before and after two 14 d periods of consuming formulated diets devoid or supplemented with fibre (14 g/l). The faecal microbiota were analysed using fluorescent in situ hybridisation and SCFA were measured using GLC. There were large and statistically significant reductions in the numbers of the Faecalibacterium prausnitzii (P < or = 0.01) and Roseburia spp. (P < or = 0.01) groups during both the fibre-free and fibre-supplemented diets. Significant and strong positive correlations between the proportion of F. prausnitzii and the proportion of butyrate during both baseline normal diets were found (pre-fibre free r 0.881, P = 0.001; pre-fibre supplemented r 0.844, P = 0.002). A significant correlation was also found between the proportional reduction in F. prausnitzii and the proportional reduction in faecal butyrate during both the fibre-free (r 0.806; P = 0.005) and the fibre-supplemented diet (r 0.749; P = 0.013). These findings may contribute to the understanding of the association between fibre, microbiota and fermentation in health, during enteral nutrition and in disease states such as Crohn's disease. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/20346190/Association_between_Faecalibacterium_prausnitzii_and_dietary_fibre_in_colonic_fermentation_in_healthy_human_subjects_ L2 - https://www.cambridge.org/core/product/identifier/S0007114510001030/type/journal_article DB - PRIME DP - Unbound Medicine ER -