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Determination of steroidal glycosides in Yucca gloriosa flowers by LC/MS/MS.
J Pharm Biomed Anal. 2010 Sep 05; 52(5):791-5.JP

Abstract

An high-performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS) method, was developed for the quantitative analysis of the steroidal glycosides occurring in Yucca gloriosa flowers. The HPLC experiments were performed by means of an octadecyl-modified reversed-phase C-18 column and a binary mobile phase system under gradient elution conditions. The fragmentation patterns of steroidal saponins were analyzed by ESI-MS(n) in positive ion mode and a specific multiple reaction monitoring MS/MS detection was developed for their quantitative determination. The described method provides high sensitivity and specificity for quantitative determination of the steroidal glycosides in Y. gloriosa flowers. Quantification was performed against an external calibration line obtained using each pure steroidal glycoside. Short- and long-term repeatabilities of the methods were better than 3 and 6%, respectively. The method was validated according to EMEA guidelines and applied to real samples.

Authors+Show Affiliations

Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, via Ponte Don Melillo, 84084 Fisciano (SA), Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

20346608

Citation

Montoro, Paola, et al. "Determination of Steroidal Glycosides in Yucca Gloriosa Flowers By LC/MS/MS." Journal of Pharmaceutical and Biomedical Analysis, vol. 52, no. 5, 2010, pp. 791-5.
Montoro P, Skhirtladze A, Perrone A, et al. Determination of steroidal glycosides in Yucca gloriosa flowers by LC/MS/MS. J Pharm Biomed Anal. 2010;52(5):791-5.
Montoro, P., Skhirtladze, A., Perrone, A., Benidze, M., Kemertelidze, E., & Piacente, S. (2010). Determination of steroidal glycosides in Yucca gloriosa flowers by LC/MS/MS. Journal of Pharmaceutical and Biomedical Analysis, 52(5), 791-5. https://doi.org/10.1016/j.jpba.2010.02.033
Montoro P, et al. Determination of Steroidal Glycosides in Yucca Gloriosa Flowers By LC/MS/MS. J Pharm Biomed Anal. 2010 Sep 5;52(5):791-5. PubMed PMID: 20346608.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of steroidal glycosides in Yucca gloriosa flowers by LC/MS/MS. AU - Montoro,Paola, AU - Skhirtladze,Alexandre, AU - Perrone,Angela, AU - Benidze,Mariam, AU - Kemertelidze,Ether, AU - Piacente,Sonia, Y1 - 2010/03/03/ PY - 2009/12/21/received PY - 2010/02/22/revised PY - 2010/02/23/accepted PY - 2010/3/30/entrez PY - 2010/3/30/pubmed PY - 2010/8/11/medline SP - 791 EP - 5 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 52 IS - 5 N2 - An high-performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS) method, was developed for the quantitative analysis of the steroidal glycosides occurring in Yucca gloriosa flowers. The HPLC experiments were performed by means of an octadecyl-modified reversed-phase C-18 column and a binary mobile phase system under gradient elution conditions. The fragmentation patterns of steroidal saponins were analyzed by ESI-MS(n) in positive ion mode and a specific multiple reaction monitoring MS/MS detection was developed for their quantitative determination. The described method provides high sensitivity and specificity for quantitative determination of the steroidal glycosides in Y. gloriosa flowers. Quantification was performed against an external calibration line obtained using each pure steroidal glycoside. Short- and long-term repeatabilities of the methods were better than 3 and 6%, respectively. The method was validated according to EMEA guidelines and applied to real samples. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/20346608/Determination_of_steroidal_glycosides_in_Yucca_gloriosa_flowers_by_LC/MS/MS_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(10)00119-6 DB - PRIME DP - Unbound Medicine ER -