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Water extract of Zanthoxylum piperitum induces vascular relaxation via endothelium-dependent NO-cGMP signaling.
J Ethnopharmacol. 2010 May 27; 129(2):197-202.JE

Abstract

AIM OF THE STUDY

The aim of the present study was to define the effects of extracts of leaves of Zanthoxylum piperitum (ZP) on the vascular tension and its mechanisms responsible in rat thoracic aortic rings.

MATERIALS AND METHODS

Methanol extract of ZP and aqueous fraction of the methanol extract (AZP) were examined for their vascular relaxant effects in isolated phenylephrine-precontracted aortic rings.

RESULTS

Methanol extract of ZP and aqueous fraction of the methanol extract (AZP) induced relaxation of the phenylephrine-precontracted aortic rings in a concentration-dependent manner. Endothelium-denudation abolished the AZP-induced vasorelaxation. Pretreatment of the endothelium-intact aortic rings with N(G)-nitro-L-arginine methylester (L-NAME) and 1H-[1,2,4]-oxadiazolo-[4,3-alpha]-quinoxalin-1-one (ODQ) inhibited the AZP-induced vasorelaxation. Inhibition of Ca(2+) entry via L-type Ca(2+) channels failed to block the AZP-induced vasorelaxation. Extracellular Ca(2+) depletion slightly but significantly attenuated the AZP-induced vasorelaxation. Thapsigargin significantly attenuated the AZP-induced vasorelaxation. Further, Gd(3+) and 2-aminoethyl diphenylborinate (2-APB), inhibitors of store-operated Ca(2+) entry (SOCE), markedly attenuated the AZP-induced vasorelaxation. Also, wortmannin, an inhibitor of Akt, an upstream signaling molecule of eNOS, attenuated the AZP-induced vasorelaxation. AZP increased cGMP levels of the aortic rings in a concentration-dependent manner and the effect was blocked by L-NAME, ODQ, thapsigargin, Gd(3+), 2-APB, and wortmannin. K(+) channel inhibition with glibenclamide and tetraethylammonium, cyclooxygenase inhibition with indomethacin, and adrenergic and muscarinic receptors blockade had no effects on the AZP-induced vasorelaxation.

CONCLUSION

Taken together, the present study suggests that AZP relaxes vascular smooth muscle via endothelium-dependent activation of NO-cGMP signaling through the Akt- and SOCE-eNOS pathways.

Authors+Show Affiliations

College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20347946

Citation

Li, Xiang, et al. "Water Extract of Zanthoxylum Piperitum Induces Vascular Relaxation Via Endothelium-dependent NO-cGMP Signaling." Journal of Ethnopharmacology, vol. 129, no. 2, 2010, pp. 197-202.
Li X, Kim HY, Cui HZ, et al. Water extract of Zanthoxylum piperitum induces vascular relaxation via endothelium-dependent NO-cGMP signaling. J Ethnopharmacol. 2010;129(2):197-202.
Li, X., Kim, H. Y., Cui, H. Z., Cho, K. W., Kang, D. G., & Lee, H. S. (2010). Water extract of Zanthoxylum piperitum induces vascular relaxation via endothelium-dependent NO-cGMP signaling. Journal of Ethnopharmacology, 129(2), 197-202. https://doi.org/10.1016/j.jep.2010.03.003
Li X, et al. Water Extract of Zanthoxylum Piperitum Induces Vascular Relaxation Via Endothelium-dependent NO-cGMP Signaling. J Ethnopharmacol. 2010 May 27;129(2):197-202. PubMed PMID: 20347946.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Water extract of Zanthoxylum piperitum induces vascular relaxation via endothelium-dependent NO-cGMP signaling. AU - Li,Xiang, AU - Kim,Hye Yoom, AU - Cui,Hao Zhen, AU - Cho,Kyung Woo, AU - Kang,Dae Gill, AU - Lee,Ho Sub, Y1 - 2010/03/27/ PY - 2009/11/10/received PY - 2010/02/25/revised PY - 2010/03/08/accepted PY - 2010/3/30/entrez PY - 2010/3/30/pubmed PY - 2010/10/29/medline SP - 197 EP - 202 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 129 IS - 2 N2 - AIM OF THE STUDY: The aim of the present study was to define the effects of extracts of leaves of Zanthoxylum piperitum (ZP) on the vascular tension and its mechanisms responsible in rat thoracic aortic rings. MATERIALS AND METHODS: Methanol extract of ZP and aqueous fraction of the methanol extract (AZP) were examined for their vascular relaxant effects in isolated phenylephrine-precontracted aortic rings. RESULTS: Methanol extract of ZP and aqueous fraction of the methanol extract (AZP) induced relaxation of the phenylephrine-precontracted aortic rings in a concentration-dependent manner. Endothelium-denudation abolished the AZP-induced vasorelaxation. Pretreatment of the endothelium-intact aortic rings with N(G)-nitro-L-arginine methylester (L-NAME) and 1H-[1,2,4]-oxadiazolo-[4,3-alpha]-quinoxalin-1-one (ODQ) inhibited the AZP-induced vasorelaxation. Inhibition of Ca(2+) entry via L-type Ca(2+) channels failed to block the AZP-induced vasorelaxation. Extracellular Ca(2+) depletion slightly but significantly attenuated the AZP-induced vasorelaxation. Thapsigargin significantly attenuated the AZP-induced vasorelaxation. Further, Gd(3+) and 2-aminoethyl diphenylborinate (2-APB), inhibitors of store-operated Ca(2+) entry (SOCE), markedly attenuated the AZP-induced vasorelaxation. Also, wortmannin, an inhibitor of Akt, an upstream signaling molecule of eNOS, attenuated the AZP-induced vasorelaxation. AZP increased cGMP levels of the aortic rings in a concentration-dependent manner and the effect was blocked by L-NAME, ODQ, thapsigargin, Gd(3+), 2-APB, and wortmannin. K(+) channel inhibition with glibenclamide and tetraethylammonium, cyclooxygenase inhibition with indomethacin, and adrenergic and muscarinic receptors blockade had no effects on the AZP-induced vasorelaxation. CONCLUSION: Taken together, the present study suggests that AZP relaxes vascular smooth muscle via endothelium-dependent activation of NO-cGMP signaling through the Akt- and SOCE-eNOS pathways. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/20347946/Water_extract_of_Zanthoxylum_piperitum_induces_vascular_relaxation_via_endothelium_dependent_NO_cGMP_signaling_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(10)00165-0 DB - PRIME DP - Unbound Medicine ER -