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Human retinal pigment epithelium-induced CD4+CD25+ regulatory T cells suppress activation of intraocular effector T cells.
Clin Immunol. 2010 Jul; 136(1):83-95.CI

Abstract

Murine retinal pigment epithelial (RPE) cells suppress T-cell activation by releasing soluble inhibitory factors and promote the generation of regulatory T cells in vitro. These T cells exposed to RPE supernatants (RPE-induced Treg cells) can suppress the activation of bystander effector T cells via the production of transforming growth factor-beta (TGFbeta). In the present study, we showed that human RPE-induced Treg cells are also able to acquire regulatory function when human RPE cell lines were pretreated with recombinant TGF beta 2. These RPE-induced Treg cells produced TGF beta 1 and IL-10 but not IFN gamma, and they significantly suppressed the activation of target cell lines and intraocular T-cell clones established from patients with active uveitis. Moreover, CD4(+)CD25(+) RPE-induced Treg cells expressed CTLA-4 and Foxp3 molecules, and the CD25(+) Treg cells profoundly suppressed the T-cell activation. Thus, in vitro manipulated Treg cells acquire functions that participate in the establishment of immune tolerance in the eye.

Authors+Show Affiliations

Department of Ophthalmology & Visual Science, Tokyo Medical and Dental University, Graduate School of Medical and Dental Sciences, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20350837

Citation

Horie, Shintaro, et al. "Human Retinal Pigment Epithelium-induced CD4+CD25+ Regulatory T Cells Suppress Activation of Intraocular Effector T Cells." Clinical Immunology (Orlando, Fla.), vol. 136, no. 1, 2010, pp. 83-95.
Horie S, Sugita S, Futagami Y, et al. Human retinal pigment epithelium-induced CD4+CD25+ regulatory T cells suppress activation of intraocular effector T cells. Clin Immunol. 2010;136(1):83-95.
Horie, S., Sugita, S., Futagami, Y., Yamada, Y., & Mochizuki, M. (2010). Human retinal pigment epithelium-induced CD4+CD25+ regulatory T cells suppress activation of intraocular effector T cells. Clinical Immunology (Orlando, Fla.), 136(1), 83-95. https://doi.org/10.1016/j.clim.2010.03.001
Horie S, et al. Human Retinal Pigment Epithelium-induced CD4+CD25+ Regulatory T Cells Suppress Activation of Intraocular Effector T Cells. Clin Immunol. 2010;136(1):83-95. PubMed PMID: 20350837.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human retinal pigment epithelium-induced CD4+CD25+ regulatory T cells suppress activation of intraocular effector T cells. AU - Horie,Shintaro, AU - Sugita,Sunao, AU - Futagami,Yuri, AU - Yamada,Yukiko, AU - Mochizuki,Manabu, Y1 - 2010/03/29/ PY - 2009/09/11/received PY - 2010/01/27/revised PY - 2010/03/02/accepted PY - 2010/3/31/entrez PY - 2010/3/31/pubmed PY - 2010/8/7/medline SP - 83 EP - 95 JF - Clinical immunology (Orlando, Fla.) JO - Clin Immunol VL - 136 IS - 1 N2 - Murine retinal pigment epithelial (RPE) cells suppress T-cell activation by releasing soluble inhibitory factors and promote the generation of regulatory T cells in vitro. These T cells exposed to RPE supernatants (RPE-induced Treg cells) can suppress the activation of bystander effector T cells via the production of transforming growth factor-beta (TGFbeta). In the present study, we showed that human RPE-induced Treg cells are also able to acquire regulatory function when human RPE cell lines were pretreated with recombinant TGF beta 2. These RPE-induced Treg cells produced TGF beta 1 and IL-10 but not IFN gamma, and they significantly suppressed the activation of target cell lines and intraocular T-cell clones established from patients with active uveitis. Moreover, CD4(+)CD25(+) RPE-induced Treg cells expressed CTLA-4 and Foxp3 molecules, and the CD25(+) Treg cells profoundly suppressed the T-cell activation. Thus, in vitro manipulated Treg cells acquire functions that participate in the establishment of immune tolerance in the eye. SN - 1521-7035 UR - https://www.unboundmedicine.com/medline/citation/20350837/Human_retinal_pigment_epithelium_induced_CD4+CD25+_regulatory_T_cells_suppress_activation_of_intraocular_effector_T_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1521-6616(10)00083-5 DB - PRIME DP - Unbound Medicine ER -