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Molecular epidemiology, sequence types, and plasmid analyses of KPC-producing Klebsiella pneumoniae strains in Israel.
Antimicrob Agents Chemother. 2010 Jul; 54(7):3002-6.AA

Abstract

Sporadic isolates of carbapenem-resistant KPC-2-producing Klebsiella pneumoniae were isolated in Tel Aviv Medical Center during 2005 and 2006, parallel to the emergence of the KPC-3-producing K. pneumoniae sequence type 258 (ST 258). We aimed to study the molecular epidemiology of these isolates and to characterize their bla(KPC)-carrying plasmids and their origin. Ten isolates (8 KPC-2 and 2 KPC-3 producing) were studied. All isolates were extremely drug resistant. They possessed the bla(KPC) gene and varied in their additional beta-lactamase contents. The KPC-2-producing strains belonged to three different sequence types: ST 340 (n = 2), ST 277 (n = 2), and a novel sequence type, ST 376 (n = 4). Among KPC-3-producing strains, a single isolate (ST 327) different from ST 258 was identified, but both strains carried the same plasmid (pKpQIL). The KPC-2-encoding plasmids varied in size (45 to 95 kb) and differed among each of the STs. Two of the Klebsiella bla(KPC-2)-carrying plasmids were identical to plasmids from Escherichia coli, suggesting a common origin of these plasmids. These data indicate that KPC evolution in K. pneumoniae is related to rare events of interspecies spread of bla(KPC-2)-carrying plasmids from E. coli followed by limited clonal spread, whereas KPC-3 carriage in this species is related almost strictly to clonal expansion of ST 258 carrying pKpQIL.

Authors+Show Affiliations

Molecular Epidemiology and Antimicrobial Resistance Laboratory, Division of Epidemiology, Tel Aviv Medical Center, Tel Aviv, Israel.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20350950

Citation

Leavitt, Azita, et al. "Molecular Epidemiology, Sequence Types, and Plasmid Analyses of KPC-producing Klebsiella Pneumoniae Strains in Israel." Antimicrobial Agents and Chemotherapy, vol. 54, no. 7, 2010, pp. 3002-6.
Leavitt A, Carmeli Y, Chmelnitsky I, et al. Molecular epidemiology, sequence types, and plasmid analyses of KPC-producing Klebsiella pneumoniae strains in Israel. Antimicrob Agents Chemother. 2010;54(7):3002-6.
Leavitt, A., Carmeli, Y., Chmelnitsky, I., Goren, M. G., Ofek, I., & Navon-Venezia, S. (2010). Molecular epidemiology, sequence types, and plasmid analyses of KPC-producing Klebsiella pneumoniae strains in Israel. Antimicrobial Agents and Chemotherapy, 54(7), 3002-6. https://doi.org/10.1128/AAC.01818-09
Leavitt A, et al. Molecular Epidemiology, Sequence Types, and Plasmid Analyses of KPC-producing Klebsiella Pneumoniae Strains in Israel. Antimicrob Agents Chemother. 2010;54(7):3002-6. PubMed PMID: 20350950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular epidemiology, sequence types, and plasmid analyses of KPC-producing Klebsiella pneumoniae strains in Israel. AU - Leavitt,Azita, AU - Carmeli,Yehuda, AU - Chmelnitsky,Inna, AU - Goren,Moran G, AU - Ofek,Itzhak, AU - Navon-Venezia,Shiri, Y1 - 2010/03/29/ PY - 2010/3/31/entrez PY - 2010/3/31/pubmed PY - 2010/9/23/medline SP - 3002 EP - 6 JF - Antimicrobial agents and chemotherapy JO - Antimicrob Agents Chemother VL - 54 IS - 7 N2 - Sporadic isolates of carbapenem-resistant KPC-2-producing Klebsiella pneumoniae were isolated in Tel Aviv Medical Center during 2005 and 2006, parallel to the emergence of the KPC-3-producing K. pneumoniae sequence type 258 (ST 258). We aimed to study the molecular epidemiology of these isolates and to characterize their bla(KPC)-carrying plasmids and their origin. Ten isolates (8 KPC-2 and 2 KPC-3 producing) were studied. All isolates were extremely drug resistant. They possessed the bla(KPC) gene and varied in their additional beta-lactamase contents. The KPC-2-producing strains belonged to three different sequence types: ST 340 (n = 2), ST 277 (n = 2), and a novel sequence type, ST 376 (n = 4). Among KPC-3-producing strains, a single isolate (ST 327) different from ST 258 was identified, but both strains carried the same plasmid (pKpQIL). The KPC-2-encoding plasmids varied in size (45 to 95 kb) and differed among each of the STs. Two of the Klebsiella bla(KPC-2)-carrying plasmids were identical to plasmids from Escherichia coli, suggesting a common origin of these plasmids. These data indicate that KPC evolution in K. pneumoniae is related to rare events of interspecies spread of bla(KPC-2)-carrying plasmids from E. coli followed by limited clonal spread, whereas KPC-3 carriage in this species is related almost strictly to clonal expansion of ST 258 carrying pKpQIL. SN - 1098-6596 UR - https://www.unboundmedicine.com/medline/citation/20350950/Molecular_epidemiology_sequence_types_and_plasmid_analyses_of_KPC_producing_Klebsiella_pneumoniae_strains_in_Israel_ L2 - http://aac.asm.org/cgi/pmidlookup?view=long&pmid=20350950 DB - PRIME DP - Unbound Medicine ER -