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The protein moiety of Brucella abortus outer membrane protein 16 is a new bacterial pathogen-associated molecular pattern that activates dendritic cells in vivo, induces a Th1 immune response, and is a promising self-adjuvanting vaccine against systemic and oral acquired brucellosis.
J Immunol. 2010 May 01; 184(9):5200-12.JI

Abstract

Knowing the inherent stimulatory properties of the lipid moiety of bacterial lipoproteins, we first hypothesized that Brucella abortus outer membrane protein (Omp)16 lipoprotein would be able to elicit a protective immune response without the need of external adjuvants. In this study, we demonstrate that Omp16 administered by the i.p. route confers significant protection against B. abortus infection and that the protective response evoked is independent of the protein lipidation. To date, Omp16 is the first Brucella protein that without the requirement of external adjuvants is able to induce similar protection levels to the control live vaccine S19. Moreover, the protein portion of Omp16 (unlipidated Omp16 [U-Omp16]) elicits a protective response when administered by the oral route. Either systemic or oral immunization with U-Omp16 elicits a Th1-specific response. These abilities of U-Omp16 indicate that it is endowed with self-adjuvanting properties. The adjuvanticity of U-Omp16 could be explained, at least in part, by its capacity to activate dendritic cells in vivo. U-Omp16 is also able to stimulate dendritic cells and macrophages in vitro. The latter property and its ability to induce a protective Th1 immune response against B. abortus infection have been found to be TLR4 dependent. The facts that U-Omp16 is an oral protective Ag and possesses a mucosal self-adjuvanting property led us to develop a plant-made vaccine expressing U-Omp16. Our results indicate that plant-expressed recombinant U-Omp16 is able to confer protective immunity, when given orally, indicating that a plant-based oral vaccine expressing U-Omp16 could be a valuable approach to controlling this disease.

Authors+Show Affiliations

Laboratory of Immunogenetics, Clinical Hospital José de San Martín, School of Medicine, University of Buenos Aires, Buenos Aires.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20351187

Citation

Pasquevich, Karina A., et al. "The Protein Moiety of Brucella Abortus Outer Membrane Protein 16 Is a New Bacterial Pathogen-associated Molecular Pattern That Activates Dendritic Cells in Vivo, Induces a Th1 Immune Response, and Is a Promising Self-adjuvanting Vaccine Against Systemic and Oral Acquired Brucellosis." Journal of Immunology (Baltimore, Md. : 1950), vol. 184, no. 9, 2010, pp. 5200-12.
Pasquevich KA, García Samartino C, Coria LM, et al. The protein moiety of Brucella abortus outer membrane protein 16 is a new bacterial pathogen-associated molecular pattern that activates dendritic cells in vivo, induces a Th1 immune response, and is a promising self-adjuvanting vaccine against systemic and oral acquired brucellosis. J Immunol. 2010;184(9):5200-12.
Pasquevich, K. A., García Samartino, C., Coria, L. M., Estein, S. M., Zwerdling, A., Ibañez, A. E., Barrionuevo, P., Oliveira, F. S., Carvalho, N. B., Borkowski, J., Oliveira, S. C., Warzecha, H., Giambartolomei, G. H., & Cassataro, J. (2010). The protein moiety of Brucella abortus outer membrane protein 16 is a new bacterial pathogen-associated molecular pattern that activates dendritic cells in vivo, induces a Th1 immune response, and is a promising self-adjuvanting vaccine against systemic and oral acquired brucellosis. Journal of Immunology (Baltimore, Md. : 1950), 184(9), 5200-12. https://doi.org/10.4049/jimmunol.0902209
Pasquevich KA, et al. The Protein Moiety of Brucella Abortus Outer Membrane Protein 16 Is a New Bacterial Pathogen-associated Molecular Pattern That Activates Dendritic Cells in Vivo, Induces a Th1 Immune Response, and Is a Promising Self-adjuvanting Vaccine Against Systemic and Oral Acquired Brucellosis. J Immunol. 2010 May 1;184(9):5200-12. PubMed PMID: 20351187.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The protein moiety of Brucella abortus outer membrane protein 16 is a new bacterial pathogen-associated molecular pattern that activates dendritic cells in vivo, induces a Th1 immune response, and is a promising self-adjuvanting vaccine against systemic and oral acquired brucellosis. AU - Pasquevich,Karina A, AU - García Samartino,Clara, AU - Coria,Lorena M, AU - Estein,Silvia M, AU - Zwerdling,Astrid, AU - Ibañez,Andrés E, AU - Barrionuevo,Paula, AU - Oliveira,Fernanda Souza de, AU - Carvalho,Natalia Barbosa, AU - Borkowski,Julia, AU - Oliveira,Sergio Costa, AU - Warzecha,Heribert, AU - Giambartolomei,Guillermo H, AU - Cassataro,Juliana, Y1 - 2010/03/29/ PY - 2010/3/31/entrez PY - 2010/3/31/pubmed PY - 2010/7/2/medline SP - 5200 EP - 12 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J. Immunol. VL - 184 IS - 9 N2 - Knowing the inherent stimulatory properties of the lipid moiety of bacterial lipoproteins, we first hypothesized that Brucella abortus outer membrane protein (Omp)16 lipoprotein would be able to elicit a protective immune response without the need of external adjuvants. In this study, we demonstrate that Omp16 administered by the i.p. route confers significant protection against B. abortus infection and that the protective response evoked is independent of the protein lipidation. To date, Omp16 is the first Brucella protein that without the requirement of external adjuvants is able to induce similar protection levels to the control live vaccine S19. Moreover, the protein portion of Omp16 (unlipidated Omp16 [U-Omp16]) elicits a protective response when administered by the oral route. Either systemic or oral immunization with U-Omp16 elicits a Th1-specific response. These abilities of U-Omp16 indicate that it is endowed with self-adjuvanting properties. The adjuvanticity of U-Omp16 could be explained, at least in part, by its capacity to activate dendritic cells in vivo. U-Omp16 is also able to stimulate dendritic cells and macrophages in vitro. The latter property and its ability to induce a protective Th1 immune response against B. abortus infection have been found to be TLR4 dependent. The facts that U-Omp16 is an oral protective Ag and possesses a mucosal self-adjuvanting property led us to develop a plant-made vaccine expressing U-Omp16. Our results indicate that plant-expressed recombinant U-Omp16 is able to confer protective immunity, when given orally, indicating that a plant-based oral vaccine expressing U-Omp16 could be a valuable approach to controlling this disease. SN - 1550-6606 UR - https://www.unboundmedicine.com/medline/citation/20351187/The_protein_moiety_of_Brucella_abortus_outer_membrane_protein_16_is_a_new_bacterial_pathogen_associated_molecular_pattern_that_activates_dendritic_cells_in_vivo_induces_a_Th1_immune_response_and_is_a_promising_self_adjuvanting_vaccine_against_systemic_and_oral_acquired_brucellosis_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=20351187 DB - PRIME DP - Unbound Medicine ER -