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Effects of lipopolysaccharide on P-glycoprotein expression and activity in the liver and kidneys.
Eur J Pharmacol. 2010 Jun 25; 636(1-3):155-8.EJ

Abstract

There have been many reports that P-glycoprotein expression and activity are altered during sepsis, but few of them have examined such changes over 72 h. In this study, we examined the effect of lipopolysaccharide (LPS, 5mg/kg, ip) on P-glycoprotein expression (Western blotting) and activity (rhodamine-123 (Rho123) pharmacokinetics) in liver and kidneys for 7 days. On day 1 after LPS administration, hepatic P-glycoprotein expression and activity significantly decreased. On day 3, hepatic P-glycoprotein expression significantly increased compared with the control group, while activity had returned to the control level. On day 7, hepatic P-glycoprotein expression returned to the control level. There were no significant changes in P-glycoprotein expression or activity in the kidneys after LPS administration. The amount of Rho123 excretion in urine remained unchanged with (4.2%) or without (4.0%) LPS administration, but the amount of Rho123 excretion in bile decreased from 2.0 to 0.7% with LPS administration. Our findings suggested that hepatic P-glycoprotein expression and activity decreased on day 1 but recovered within 3 days, but there were no significant differences in the kidneys after LPS administration. These results suggested that the change in P-glycoprotein activity might be due to change in P-glycoprotein expression in the liver rather than the kidneys.

Authors+Show Affiliations

Department of Clinical Pharmacy and Clinical Pharmacokinetics, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takastuki, Osaka 569-1094, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20353768

Citation

Kato, Ryuji, et al. "Effects of Lipopolysaccharide On P-glycoprotein Expression and Activity in the Liver and Kidneys." European Journal of Pharmacology, vol. 636, no. 1-3, 2010, pp. 155-8.
Kato R, Moriguchi J, Irie T, et al. Effects of lipopolysaccharide on P-glycoprotein expression and activity in the liver and kidneys. Eur J Pharmacol. 2010;636(1-3):155-8.
Kato, R., Moriguchi, J., Irie, T., Nakagawa, M., Kusukawa, Y., Matsumura, H., Ijiri, Y., & Tanaka, K. (2010). Effects of lipopolysaccharide on P-glycoprotein expression and activity in the liver and kidneys. European Journal of Pharmacology, 636(1-3), 155-8. https://doi.org/10.1016/j.ejphar.2010.03.024
Kato R, et al. Effects of Lipopolysaccharide On P-glycoprotein Expression and Activity in the Liver and Kidneys. Eur J Pharmacol. 2010 Jun 25;636(1-3):155-8. PubMed PMID: 20353768.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of lipopolysaccharide on P-glycoprotein expression and activity in the liver and kidneys. AU - Kato,Ryuji, AU - Moriguchi,Jun, AU - Irie,Takako, AU - Nakagawa,Machiko, AU - Kusukawa,Yugo, AU - Matsumura,Hitoshi, AU - Ijiri,Yoshio, AU - Tanaka,Kazuhiko, Y1 - 2010/03/29/ PY - 2009/08/18/received PY - 2010/02/25/revised PY - 2010/03/12/accepted PY - 2010/4/1/entrez PY - 2010/4/1/pubmed PY - 2010/8/10/medline SP - 155 EP - 8 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 636 IS - 1-3 N2 - There have been many reports that P-glycoprotein expression and activity are altered during sepsis, but few of them have examined such changes over 72 h. In this study, we examined the effect of lipopolysaccharide (LPS, 5mg/kg, ip) on P-glycoprotein expression (Western blotting) and activity (rhodamine-123 (Rho123) pharmacokinetics) in liver and kidneys for 7 days. On day 1 after LPS administration, hepatic P-glycoprotein expression and activity significantly decreased. On day 3, hepatic P-glycoprotein expression significantly increased compared with the control group, while activity had returned to the control level. On day 7, hepatic P-glycoprotein expression returned to the control level. There were no significant changes in P-glycoprotein expression or activity in the kidneys after LPS administration. The amount of Rho123 excretion in urine remained unchanged with (4.2%) or without (4.0%) LPS administration, but the amount of Rho123 excretion in bile decreased from 2.0 to 0.7% with LPS administration. Our findings suggested that hepatic P-glycoprotein expression and activity decreased on day 1 but recovered within 3 days, but there were no significant differences in the kidneys after LPS administration. These results suggested that the change in P-glycoprotein activity might be due to change in P-glycoprotein expression in the liver rather than the kidneys. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/20353768/Effects_of_lipopolysaccharide_on_P_glycoprotein_expression_and_activity_in_the_liver_and_kidneys_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(10)00220-7 DB - PRIME DP - Unbound Medicine ER -