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Gender-dependent effect of Gpbar1 genetic deletion on the metabolic profiles of diet-induced obese mice.
J Endocrinol. 2010 Jun; 205(3):225-32.JE

Abstract

G-protein-coupled bile acid receptor 1 (GPBAR1/TGR5/M-Bar/GPR131) is a cell surface receptor involved in the regulation of bile acid metabolism. We have previously shown that Gpbar1-null mice are resistant to cholesterol gallstone disease when fed a lithogenic diet. Other published studies have suggested that Gpbar1 is involved in both energy homeostasis and glucose homeostasis. Here, we examine the functional role of Gpbar1 in diet-induced obese mice. We found that body weight, food intake, and fasted blood glucose levels were similar between Gpbar1-null mice and their wild-type (WT) littermates when fed a chow or high-fat diet (HFD) for 2 months. However, insulin tolerance tests revealed improved insulin sensitivity in male Gpbar1(-/-) mice fed chow, but impaired insulin sensitivity when fed a HFD. In contrast, female Gpbar1(-/-) mice exhibited improved insulin sensitivity when fed a HFD compared with their WT littermates. Female Gpbar1(-/-) mice had significantly lower plasma cholesterol and triglyceride levels than their WT littermates on both diets. Male Gpbar1(-/-) mice on HFD displayed increased hepatic steatosis when compared with Gpbar1(+)(/)(+) males and Gpbar1(-/-) females on HFD. These results suggest a gender-dependent regulation of Gpbar1 function in metabolic disease.

Authors+Show Affiliations

Merck Research Laboratories, Department of Discovery Technologies, Kenilworth, New Jersey 07033, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20354075

Citation

Vassileva, Galya, et al. "Gender-dependent Effect of Gpbar1 Genetic Deletion On the Metabolic Profiles of Diet-induced Obese Mice." The Journal of Endocrinology, vol. 205, no. 3, 2010, pp. 225-32.
Vassileva G, Hu W, Hoos L, et al. Gender-dependent effect of Gpbar1 genetic deletion on the metabolic profiles of diet-induced obese mice. J Endocrinol. 2010;205(3):225-32.
Vassileva, G., Hu, W., Hoos, L., Tetzloff, G., Yang, S., Liu, L., Kang, L., Davis, H. R., Hedrick, J. A., Lan, H., Kowalski, T., & Gustafson, E. L. (2010). Gender-dependent effect of Gpbar1 genetic deletion on the metabolic profiles of diet-induced obese mice. The Journal of Endocrinology, 205(3), 225-32. https://doi.org/10.1677/JOE-10-0009
Vassileva G, et al. Gender-dependent Effect of Gpbar1 Genetic Deletion On the Metabolic Profiles of Diet-induced Obese Mice. J Endocrinol. 2010;205(3):225-32. PubMed PMID: 20354075.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gender-dependent effect of Gpbar1 genetic deletion on the metabolic profiles of diet-induced obese mice. AU - Vassileva,Galya, AU - Hu,Weiwen, AU - Hoos,Lizbeth, AU - Tetzloff,Glen, AU - Yang,Shijun, AU - Liu,Li, AU - Kang,Ling, AU - Davis,Harry R, AU - Hedrick,Joseph A, AU - Lan,Hong, AU - Kowalski,Timothy, AU - Gustafson,Eric L, Y1 - 2010/03/30/ PY - 2010/4/1/entrez PY - 2010/4/1/pubmed PY - 2010/6/4/medline SP - 225 EP - 32 JF - The Journal of endocrinology JO - J. Endocrinol. VL - 205 IS - 3 N2 - G-protein-coupled bile acid receptor 1 (GPBAR1/TGR5/M-Bar/GPR131) is a cell surface receptor involved in the regulation of bile acid metabolism. We have previously shown that Gpbar1-null mice are resistant to cholesterol gallstone disease when fed a lithogenic diet. Other published studies have suggested that Gpbar1 is involved in both energy homeostasis and glucose homeostasis. Here, we examine the functional role of Gpbar1 in diet-induced obese mice. We found that body weight, food intake, and fasted blood glucose levels were similar between Gpbar1-null mice and their wild-type (WT) littermates when fed a chow or high-fat diet (HFD) for 2 months. However, insulin tolerance tests revealed improved insulin sensitivity in male Gpbar1(-/-) mice fed chow, but impaired insulin sensitivity when fed a HFD. In contrast, female Gpbar1(-/-) mice exhibited improved insulin sensitivity when fed a HFD compared with their WT littermates. Female Gpbar1(-/-) mice had significantly lower plasma cholesterol and triglyceride levels than their WT littermates on both diets. Male Gpbar1(-/-) mice on HFD displayed increased hepatic steatosis when compared with Gpbar1(+)(/)(+) males and Gpbar1(-/-) females on HFD. These results suggest a gender-dependent regulation of Gpbar1 function in metabolic disease. SN - 1479-6805 UR - https://www.unboundmedicine.com/medline/citation/20354075/Gender_dependent_effect_of_Gpbar1_genetic_deletion_on_the_metabolic_profiles_of_diet_induced_obese_mice_ L2 - https://joe.bioscientifica.com/doi/10.1677/JOE-10-0009 DB - PRIME DP - Unbound Medicine ER -