Tags

Type your tag names separated by a space and hit enter

Simultaneous determination of procaine, lidocaine, ropivacaine, tetracaine and bupivacaine in human plasma by high-performance liquid chromatography.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 May 01; 878(15-16):1185-9.JC

Abstract

A simple and sensitive high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method has been developed and validated for simultaneous quantification of five local anesthetics in human plasma: procaine, lidocaine, ropivacaine, tetracaine and bupivacaine. In an ice-water bath, 500 microL plasma sample, containing 100 microg/mL neostigmine methylsulfate as anticholinesterase, was spiked with carbamazepine as internal standard and alkalized by sodium hydroxide. Liquid-liquid extraction with ethyl ether was used for plasma sample preparation. The chromatographic separation was achieved on a Kromosil ODS C18 column with a mobile phase consisting of 30 mM potassium dihydrogen phosphate buffer (0.16% triethylamine, pH adjusted to 4.9 with phosphoric acid) and acetonitrile (63/37, v/v). The detection was performed simultaneously at wavelengths of 210 and 290 nm. The chromatographic analysis time was 13 min per sample. The calibration curves of all five analytes were linear between 0.05 and 5.0 microg/mL (r(2) > or = 0.998). Precision ranged from 1.4% to 7.9% and accuracy was between 91.7% and 106.5%. The validated method is applicable for simultaneous determination of procaine, lidocaine, ropivacaine, tetracaine and bupivacaine for therapeutic drug monitoring and pharmacokinetic study.

Authors+Show Affiliations

Clinical Pharmacy Laboratory, Huashan Hospital, Fudan University, 12 Wu Lu Mu Qi M Rd, Shanghai 200040, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20356808

Citation

Qin, Wei-Wei, et al. "Simultaneous Determination of Procaine, Lidocaine, Ropivacaine, Tetracaine and Bupivacaine in Human Plasma By High-performance Liquid Chromatography." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 878, no. 15-16, 2010, pp. 1185-9.
Qin WW, Jiao Z, Zhong MK, et al. Simultaneous determination of procaine, lidocaine, ropivacaine, tetracaine and bupivacaine in human plasma by high-performance liquid chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2010;878(15-16):1185-9.
Qin, W. W., Jiao, Z., Zhong, M. K., Shi, X. J., Zhang, J., Li, Z. D., & Cui, X. Y. (2010). Simultaneous determination of procaine, lidocaine, ropivacaine, tetracaine and bupivacaine in human plasma by high-performance liquid chromatography. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 878(15-16), 1185-9. https://doi.org/10.1016/j.jchromb.2010.03.003
Qin WW, et al. Simultaneous Determination of Procaine, Lidocaine, Ropivacaine, Tetracaine and Bupivacaine in Human Plasma By High-performance Liquid Chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2010 May 1;878(15-16):1185-9. PubMed PMID: 20356808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of procaine, lidocaine, ropivacaine, tetracaine and bupivacaine in human plasma by high-performance liquid chromatography. AU - Qin,Wei-Wei, AU - Jiao,Zheng, AU - Zhong,Ming-Kang, AU - Shi,Xiao-Jin, AU - Zhang,Jun, AU - Li,Zhong-Dong, AU - Cui,Xue-Yan, Y1 - 2010/03/11/ PY - 2009/11/15/received PY - 2010/02/26/revised PY - 2010/03/04/accepted PY - 2010/4/2/entrez PY - 2010/4/2/pubmed PY - 2010/8/3/medline SP - 1185 EP - 9 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 878 IS - 15-16 N2 - A simple and sensitive high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method has been developed and validated for simultaneous quantification of five local anesthetics in human plasma: procaine, lidocaine, ropivacaine, tetracaine and bupivacaine. In an ice-water bath, 500 microL plasma sample, containing 100 microg/mL neostigmine methylsulfate as anticholinesterase, was spiked with carbamazepine as internal standard and alkalized by sodium hydroxide. Liquid-liquid extraction with ethyl ether was used for plasma sample preparation. The chromatographic separation was achieved on a Kromosil ODS C18 column with a mobile phase consisting of 30 mM potassium dihydrogen phosphate buffer (0.16% triethylamine, pH adjusted to 4.9 with phosphoric acid) and acetonitrile (63/37, v/v). The detection was performed simultaneously at wavelengths of 210 and 290 nm. The chromatographic analysis time was 13 min per sample. The calibration curves of all five analytes were linear between 0.05 and 5.0 microg/mL (r(2) > or = 0.998). Precision ranged from 1.4% to 7.9% and accuracy was between 91.7% and 106.5%. The validated method is applicable for simultaneous determination of procaine, lidocaine, ropivacaine, tetracaine and bupivacaine for therapeutic drug monitoring and pharmacokinetic study. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/20356808/Simultaneous_determination_of_procaine_lidocaine_ropivacaine_tetracaine_and_bupivacaine_in_human_plasma_by_high_performance_liquid_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(10)00137-6 DB - PRIME DP - Unbound Medicine ER -