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Prokaryotic community structure and diversity in the sediments of an active submarine mud volcano (Kazan mud volcano, East Mediterranean Sea).
FEMS Microbiol Ecol. 2010 Jun; 72(3):429-44.FM

Abstract

We investigated 16S rRNA gene diversity at a high sediment depth resolution (every 5 cm, top 30 cm) in an active site of the Kazan mud volcano, East Mediterranean Sea. A total of 242 archaeal and 374 bacterial clones were analysed, which were attributed to 38 and 205 unique phylotypes, respectively (> or = 98% similarity). Most of the archaeal phylotypes were related to ANME-1, -2 and -3 members originating from habitats where anaerobic oxidation of methane (AOM) occurs, although they occurred in sediment layers with no apparent AOM (below the sulphate depletion depth). Proteobacteria were the most abundant and diverse bacterial group, with the Gammaproteobacteria dominating in most sediment layers and these were related to phylotypes involved in methane cycling. The Deltaproteobacteria included several of the sulphate-reducers related to AOM. The rest of the bacterial phylotypes belonged to 15 known phyla and three unaffiliated groups, with representatives from similar habitats. Diversity index H was in the range 0.56-1.73 and 1.47-3.82 for Archaea and Bacteria, respectively, revealing different depth patterns for the two groups. At 15 and 20 cm below the sea floor, the prokaryotic communities were highly similar, hosting AOM-specific Archaea and Bacteria. Our study revealed different dominant phyla in proximate sediment layers.

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Authors+Show Affiliations

Pachiadaki MG
Environmental Chemical Processes Laboratory, Department of Chemistry, University of Crete, Voutes-Heraklion, Greece.
Lykousis V
No affiliation info available
Stefanou EG
No affiliation info available
Kormas KA
No affiliation info available

MeSH

ArchaeaBacteriaBiodiversityDNA, ArchaealDNA, BacterialGeologic SedimentsMediterranean SeaMethanePhylogenyRNA, Ribosomal, 16SSequence Analysis, DNAWater Microbiology

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20370830

Citation

Pachiadaki, Maria G., et al. "Prokaryotic Community Structure and Diversity in the Sediments of an Active Submarine Mud Volcano (Kazan Mud Volcano, East Mediterranean Sea)." FEMS Microbiology Ecology, vol. 72, no. 3, 2010, pp. 429-44.
Pachiadaki MG, Lykousis V, Stefanou EG, et al. Prokaryotic community structure and diversity in the sediments of an active submarine mud volcano (Kazan mud volcano, East Mediterranean Sea). FEMS Microbiol Ecol. 2010;72(3):429-44.
Pachiadaki, M. G., Lykousis, V., Stefanou, E. G., & Kormas, K. A. (2010). Prokaryotic community structure and diversity in the sediments of an active submarine mud volcano (Kazan mud volcano, East Mediterranean Sea). FEMS Microbiology Ecology, 72(3), 429-44. https://doi.org/10.1111/j.1574-6941.2010.00857.x
Pachiadaki MG, et al. Prokaryotic Community Structure and Diversity in the Sediments of an Active Submarine Mud Volcano (Kazan Mud Volcano, East Mediterranean Sea). FEMS Microbiol Ecol. 2010;72(3):429-44. PubMed PMID: 20370830.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prokaryotic community structure and diversity in the sediments of an active submarine mud volcano (Kazan mud volcano, East Mediterranean Sea). AU - Pachiadaki,Maria G, AU - Lykousis,Vasilios, AU - Stefanou,Euripides G, AU - Kormas,Konstantinos A, Y1 - 2010/03/03/ PY - 2010/4/8/entrez PY - 2010/4/8/pubmed PY - 2010/9/15/medline SP - 429 EP - 44 JF - FEMS microbiology ecology JO - FEMS Microbiol Ecol VL - 72 IS - 3 N2 - We investigated 16S rRNA gene diversity at a high sediment depth resolution (every 5 cm, top 30 cm) in an active site of the Kazan mud volcano, East Mediterranean Sea. A total of 242 archaeal and 374 bacterial clones were analysed, which were attributed to 38 and 205 unique phylotypes, respectively (> or = 98% similarity). Most of the archaeal phylotypes were related to ANME-1, -2 and -3 members originating from habitats where anaerobic oxidation of methane (AOM) occurs, although they occurred in sediment layers with no apparent AOM (below the sulphate depletion depth). Proteobacteria were the most abundant and diverse bacterial group, with the Gammaproteobacteria dominating in most sediment layers and these were related to phylotypes involved in methane cycling. The Deltaproteobacteria included several of the sulphate-reducers related to AOM. The rest of the bacterial phylotypes belonged to 15 known phyla and three unaffiliated groups, with representatives from similar habitats. Diversity index H was in the range 0.56-1.73 and 1.47-3.82 for Archaea and Bacteria, respectively, revealing different depth patterns for the two groups. At 15 and 20 cm below the sea floor, the prokaryotic communities were highly similar, hosting AOM-specific Archaea and Bacteria. Our study revealed different dominant phyla in proximate sediment layers. SN - 1574-6941 UR - https://www.unboundmedicine.com/medline/citation/20370830/Prokaryotic_community_structure_and_diversity_in_the_sediments_of_an_active_submarine_mud_volcano__Kazan_mud_volcano_East_Mediterranean_Sea__ L2 - https://academic.oup.com/femsec/article-lookup/doi/10.1111/j.1574-6941.2010.00857.x DB - PRIME DP - Unbound Medicine ER -