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Validated liquid chromatography-tandem mass spectrometry method for quantitative determination of dauricine in human plasma and its application to pharmacokinetic study.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 May 01; 878(15-16):1199-203.JC

Abstract

A highly sensitive and selective LC-MS/MS method was developed and validated for the determination of dauricine in human plasma, using protopine as internal standard (IS). The analyte and IS were extracted by liquid-liquid extraction and analyzed by LC-MS/MS. Chromatographic separation was performed on Agilent TC-C(18) column with a mobile phase of methanol-water-glacial acetic acid (60:40:0.8, v/v/v) at a flow rate of 0.7 mL/min. Detection was performed on a triple quadrupole tandem mass spectrum by multiple reaction monitoring (MRM) mode using the electrospray ionization technique in positive mode. The method was linear over the concentration range of 1-200 ng/mL. The lower limit of quantification (LLOQ) was 1 ng/mL in human plasma with acceptable precision and accuracy. The intra- and inter-day precision was less than 5.9% determined from quality control (QC) samples at concentrations of 2.0, 20.0 and 160 ng/mL, and the accuracy was within +/-9.9%. This method was successfully applied for the evaluation of pharmacokinetics of dauricine after oral doses of 100, 300 and 600 mg phenolic alkaloids of menispermum dauricum tablet (PAMDT) to 12 Chinese healthy volunteers.

Authors+Show Affiliations

School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20371215

Citation

Liu, Xiaoying, et al. "Validated Liquid Chromatography-tandem Mass Spectrometry Method for Quantitative Determination of Dauricine in Human Plasma and Its Application to Pharmacokinetic Study." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 878, no. 15-16, 2010, pp. 1199-203.
Liu X, Liu Q, Wang D, et al. Validated liquid chromatography-tandem mass spectrometry method for quantitative determination of dauricine in human plasma and its application to pharmacokinetic study. J Chromatogr B Analyt Technol Biomed Life Sci. 2010;878(15-16):1199-203.
Liu, X., Liu, Q., Wang, D., Wang, X., Zhang, P., Xu, H., Zhao, H., & Zhao, H. (2010). Validated liquid chromatography-tandem mass spectrometry method for quantitative determination of dauricine in human plasma and its application to pharmacokinetic study. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 878(15-16), 1199-203. https://doi.org/10.1016/j.jchromb.2010.03.028
Liu X, et al. Validated Liquid Chromatography-tandem Mass Spectrometry Method for Quantitative Determination of Dauricine in Human Plasma and Its Application to Pharmacokinetic Study. J Chromatogr B Analyt Technol Biomed Life Sci. 2010 May 1;878(15-16):1199-203. PubMed PMID: 20371215.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validated liquid chromatography-tandem mass spectrometry method for quantitative determination of dauricine in human plasma and its application to pharmacokinetic study. AU - Liu,Xiaoying, AU - Liu,Qian, AU - Wang,Dongmei, AU - Wang,Xueya, AU - Zhang,Peng, AU - Xu,Haiyan, AU - Zhao,Hui, AU - Zhao,Huaiqing, Y1 - 2010/03/20/ PY - 2009/11/20/received PY - 2010/03/13/revised PY - 2010/03/15/accepted PY - 2010/4/8/entrez PY - 2010/4/8/pubmed PY - 2010/8/3/medline SP - 1199 EP - 203 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 878 IS - 15-16 N2 - A highly sensitive and selective LC-MS/MS method was developed and validated for the determination of dauricine in human plasma, using protopine as internal standard (IS). The analyte and IS were extracted by liquid-liquid extraction and analyzed by LC-MS/MS. Chromatographic separation was performed on Agilent TC-C(18) column with a mobile phase of methanol-water-glacial acetic acid (60:40:0.8, v/v/v) at a flow rate of 0.7 mL/min. Detection was performed on a triple quadrupole tandem mass spectrum by multiple reaction monitoring (MRM) mode using the electrospray ionization technique in positive mode. The method was linear over the concentration range of 1-200 ng/mL. The lower limit of quantification (LLOQ) was 1 ng/mL in human plasma with acceptable precision and accuracy. The intra- and inter-day precision was less than 5.9% determined from quality control (QC) samples at concentrations of 2.0, 20.0 and 160 ng/mL, and the accuracy was within +/-9.9%. This method was successfully applied for the evaluation of pharmacokinetics of dauricine after oral doses of 100, 300 and 600 mg phenolic alkaloids of menispermum dauricum tablet (PAMDT) to 12 Chinese healthy volunteers. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/20371215/Validated_liquid_chromatography_tandem_mass_spectrometry_method_for_quantitative_determination_of_dauricine_in_human_plasma_and_its_application_to_pharmacokinetic_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(10)00168-6 DB - PRIME DP - Unbound Medicine ER -