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Development of protection in rainbow trout (Oncorhynchus mykiss, Walbaum) to Vibrio anguillarum following use of the probiotic Kocuria SM1.
Fish Shellfish Immunol. 2010 Aug; 29(2):212-6.FS

Abstract

Probiotic Kocuria SM1, which was part of the allochthonous gut microbiota of rainbow trout, was administered to rainbow trout (Oncorhynchus mykiss) fingerlings as dietary supplement at approximately 10(8) cells g(-1) for two weeks. The fish were challenged with Vibrio anguillarum and innate immunity determined over the following 5 weeks. The probiotic-fed fish survived (mortalities = 10-28%; P < 0.05) better than the controls (mortalities = 73-92%) for 5 weeks after stopping administration of Kocuria SM1, although the relative percent survival (RPS) declined steadily from 87 to 36% by the end of the experiment. A significant (P < 0.05) effect on the immune parameters was observed in fish within 3 weeks after stopping administration of Kocuria SM1 in comparison with the controls, i.e. enhancement of the respiratory burst (OD(550nm)) from 0.051 +/- 0.014 to 0.067 +/- 0.009, lysozyme 605 +/- 185 units ml(-1) to 872 +/- 114 units ml(-1), total protein 19.8 +/- 2.0 mg ml(-1) to 23.8 +/- 0.8 mg ml(-1) at 2 weeks, and leukocrit 2.9 +/- 1.8% to 5.1 +/- 1.1%, peroxidase (OD(550nm)) 0.23 +/- 0.02 to 0.37 +/- 0.11 and bacterial killing activities (percentage of surviving bacteria = 82 +/- 12% to 57 +/- 4%) at 3 weeks. Generally, the levels reduced progressively towards the end of experiment.

Authors+Show Affiliations

School of Life Sciences, Heriot-Watt University, Edinburgh EH14 4AS, Scotland, UK.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20371293

Citation

Sharifuzzaman, S M., and B Austin. "Development of Protection in Rainbow Trout (Oncorhynchus Mykiss, Walbaum) to Vibrio Anguillarum Following Use of the Probiotic Kocuria SM1." Fish & Shellfish Immunology, vol. 29, no. 2, 2010, pp. 212-6.
Sharifuzzaman SM, Austin B. Development of protection in rainbow trout (Oncorhynchus mykiss, Walbaum) to Vibrio anguillarum following use of the probiotic Kocuria SM1. Fish Shellfish Immunol. 2010;29(2):212-6.
Sharifuzzaman, S. M., & Austin, B. (2010). Development of protection in rainbow trout (Oncorhynchus mykiss, Walbaum) to Vibrio anguillarum following use of the probiotic Kocuria SM1. Fish & Shellfish Immunology, 29(2), 212-6. https://doi.org/10.1016/j.fsi.2010.03.008
Sharifuzzaman SM, Austin B. Development of Protection in Rainbow Trout (Oncorhynchus Mykiss, Walbaum) to Vibrio Anguillarum Following Use of the Probiotic Kocuria SM1. Fish Shellfish Immunol. 2010;29(2):212-6. PubMed PMID: 20371293.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of protection in rainbow trout (Oncorhynchus mykiss, Walbaum) to Vibrio anguillarum following use of the probiotic Kocuria SM1. AU - Sharifuzzaman,S M, AU - Austin,B, Y1 - 2010/04/03/ PY - 2010/01/20/received PY - 2010/03/15/revised PY - 2010/03/26/accepted PY - 2010/4/8/entrez PY - 2010/4/8/pubmed PY - 2010/11/10/medline SP - 212 EP - 6 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 29 IS - 2 N2 - Probiotic Kocuria SM1, which was part of the allochthonous gut microbiota of rainbow trout, was administered to rainbow trout (Oncorhynchus mykiss) fingerlings as dietary supplement at approximately 10(8) cells g(-1) for two weeks. The fish were challenged with Vibrio anguillarum and innate immunity determined over the following 5 weeks. The probiotic-fed fish survived (mortalities = 10-28%; P < 0.05) better than the controls (mortalities = 73-92%) for 5 weeks after stopping administration of Kocuria SM1, although the relative percent survival (RPS) declined steadily from 87 to 36% by the end of the experiment. A significant (P < 0.05) effect on the immune parameters was observed in fish within 3 weeks after stopping administration of Kocuria SM1 in comparison with the controls, i.e. enhancement of the respiratory burst (OD(550nm)) from 0.051 +/- 0.014 to 0.067 +/- 0.009, lysozyme 605 +/- 185 units ml(-1) to 872 +/- 114 units ml(-1), total protein 19.8 +/- 2.0 mg ml(-1) to 23.8 +/- 0.8 mg ml(-1) at 2 weeks, and leukocrit 2.9 +/- 1.8% to 5.1 +/- 1.1%, peroxidase (OD(550nm)) 0.23 +/- 0.02 to 0.37 +/- 0.11 and bacterial killing activities (percentage of surviving bacteria = 82 +/- 12% to 57 +/- 4%) at 3 weeks. Generally, the levels reduced progressively towards the end of experiment. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/20371293/Development_of_protection_in_rainbow_trout__Oncorhynchus_mykiss_Walbaum__to_Vibrio_anguillarum_following_use_of_the_probiotic_Kocuria_SM1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(10)00098-7 DB - PRIME DP - Unbound Medicine ER -