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How patchy is patchy villous atrophy?: distribution pattern of histological lesions in the duodenum of children with celiac disease.
Am J Gastroenterol. 2010 Sep; 105(9):2103-10.AJ

Abstract

OBJECTIVES

In celiac disease (CD) the degree of histological damage in the duodenum may vary, but there is some controversy about the coexistence of villous atrophy and normal mucosa in different biopsy sites, i.e., patchy villous atrophy. We prospectively evaluated the degree, frequency, and distribution of histological lesions among different duodenal sites as well as within each duodenal biopsy.

METHODS

Over the last 4 years, in each patient with suspected CD (positive anti-transglutaminase antibodies), four to five endoscopic biopsies were taken from the duodeno-jejunal flexure/distal duodenum (D3), intermediate duodenum (D2), proximal duodenum (D1), and duodenal bulb (B). Biopsies were subjected to hematoxylin/eosin staining and immunostaining with anti-CD3 monoclonal antibodies for intraepithelial lymphocyte (IEL) count. Duodenal lesions were classified according to Marsh-Oberhuber, and CD was diagnosed according to the European Society of Pediatric Gastroenterology, Hepatology, and Nutrition criteria.

RESULTS

Six hundred and eighty-six children did have CD. A degree of villous atrophy was found in 660/686 patients (96.2%), total villous atrophy was present in 550/686 (80.1%), and 320/686 (46.6%) had different lesions at different sites, but none of these patients had entirely normal biopsies. In all, 116 of 686 (16.9%) had variable lesions within the same biopsy, with grade 2+3A being the most frequent association (43%), followed by 2+3A+3B (27%) and 2+3A+3B+3C (22%). All these 116 patients also had histologically normal areas within the same biopsy, but anti-CD3 immunostaining showed that IELs were always increased in such areas. In all the cases, the severity of duodenal lesions significantly increased in an aborad manner (chi(2)=52.38 with alpha=0.01 and d.f.=12; P<0.0001). No correlation was found between type and distribution of histologic lesions and clinical presentation of CD.

CONCLUSIONS

In newly diagnosed CD, some variability of histological lesions can be found, even within the same duodenal biopsy, in which areas of apparently normal mucosa with increased IEL number often exist. We also confirm our previous findings that duodenal lesions may vary among different biopsies; lesion severity has a proximal-to-distal gradient, but no patient has entirely normal duodenal biopsies. The awareness of such histological variability may help establish a correct diagnosis of CD.

Authors+Show Affiliations

Gastrointestinal Pathophysiology and Endoscopy, University Department of Pediatrics, Children's Hospital, Spedali Civili, Brescia, Italy. alberto_ravelli@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20372112

Citation

Ravelli, Alberto, et al. "How Patchy Is Patchy Villous Atrophy?: Distribution Pattern of Histological Lesions in the Duodenum of Children With Celiac Disease." The American Journal of Gastroenterology, vol. 105, no. 9, 2010, pp. 2103-10.
Ravelli A, Villanacci V, Monfredini C, et al. How patchy is patchy villous atrophy?: distribution pattern of histological lesions in the duodenum of children with celiac disease. Am J Gastroenterol. 2010;105(9):2103-10.
Ravelli, A., Villanacci, V., Monfredini, C., Martinazzi, S., Grassi, V., & Manenti, S. (2010). How patchy is patchy villous atrophy?: distribution pattern of histological lesions in the duodenum of children with celiac disease. The American Journal of Gastroenterology, 105(9), 2103-10. https://doi.org/10.1038/ajg.2010.153
Ravelli A, et al. How Patchy Is Patchy Villous Atrophy?: Distribution Pattern of Histological Lesions in the Duodenum of Children With Celiac Disease. Am J Gastroenterol. 2010;105(9):2103-10. PubMed PMID: 20372112.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - How patchy is patchy villous atrophy?: distribution pattern of histological lesions in the duodenum of children with celiac disease. AU - Ravelli,Alberto, AU - Villanacci,Vincenzo, AU - Monfredini,Chiara, AU - Martinazzi,Silvia, AU - Grassi,Veronica, AU - Manenti,Stefania, Y1 - 2010/04/06/ PY - 2010/4/8/entrez PY - 2010/4/8/pubmed PY - 2010/9/29/medline SP - 2103 EP - 10 JF - The American journal of gastroenterology JO - Am J Gastroenterol VL - 105 IS - 9 N2 - OBJECTIVES: In celiac disease (CD) the degree of histological damage in the duodenum may vary, but there is some controversy about the coexistence of villous atrophy and normal mucosa in different biopsy sites, i.e., patchy villous atrophy. We prospectively evaluated the degree, frequency, and distribution of histological lesions among different duodenal sites as well as within each duodenal biopsy. METHODS: Over the last 4 years, in each patient with suspected CD (positive anti-transglutaminase antibodies), four to five endoscopic biopsies were taken from the duodeno-jejunal flexure/distal duodenum (D3), intermediate duodenum (D2), proximal duodenum (D1), and duodenal bulb (B). Biopsies were subjected to hematoxylin/eosin staining and immunostaining with anti-CD3 monoclonal antibodies for intraepithelial lymphocyte (IEL) count. Duodenal lesions were classified according to Marsh-Oberhuber, and CD was diagnosed according to the European Society of Pediatric Gastroenterology, Hepatology, and Nutrition criteria. RESULTS: Six hundred and eighty-six children did have CD. A degree of villous atrophy was found in 660/686 patients (96.2%), total villous atrophy was present in 550/686 (80.1%), and 320/686 (46.6%) had different lesions at different sites, but none of these patients had entirely normal biopsies. In all, 116 of 686 (16.9%) had variable lesions within the same biopsy, with grade 2+3A being the most frequent association (43%), followed by 2+3A+3B (27%) and 2+3A+3B+3C (22%). All these 116 patients also had histologically normal areas within the same biopsy, but anti-CD3 immunostaining showed that IELs were always increased in such areas. In all the cases, the severity of duodenal lesions significantly increased in an aborad manner (chi(2)=52.38 with alpha=0.01 and d.f.=12; P<0.0001). No correlation was found between type and distribution of histologic lesions and clinical presentation of CD. CONCLUSIONS: In newly diagnosed CD, some variability of histological lesions can be found, even within the same duodenal biopsy, in which areas of apparently normal mucosa with increased IEL number often exist. We also confirm our previous findings that duodenal lesions may vary among different biopsies; lesion severity has a proximal-to-distal gradient, but no patient has entirely normal duodenal biopsies. The awareness of such histological variability may help establish a correct diagnosis of CD. SN - 1572-0241 UR - https://www.unboundmedicine.com/medline/citation/20372112/How_patchy_is_patchy_villous_atrophy:_distribution_pattern_of_histological_lesions_in_the_duodenum_of_children_with_celiac_disease_ L2 - https://Insights.ovid.com/pubmed?pmid=20372112 DB - PRIME DP - Unbound Medicine ER -