Tags

Type your tag names separated by a space and hit enter

Correlation between the VITEK2 system and cefoxitin disk diffusion for the daily detection of oxacillin resistance in a large number of clinical Staphylococcus aureus isolates.
Eur J Clin Microbiol Infect Dis. 2010 Jun; 29(6):745-7.EJ

Abstract

The aim of the present study was to compare the performance of the new VITEK2 AST-P551 card with the cefoxitin disk diffusion method for the daily detection of methicillin resistance with a high number of Staphylococcus aureus clinical isolates. Detection of the PBP2a protein or mecA gene was performed for each discordant case. Seventy (3.3%) isolates out of 2,107 clinical strains showed discordant results, two very major errors, four major errors and 64 minor errors. Fifty-nine (84%) discordant results were resolved, with a final overall agreement of 99.5%. Eleven (0.5%) strains remained discordant (minor error [mE]). Four of 370 MRSA strains were misclassified as susceptible in daily practice by the cefoxitin disk diffusion method. All of these strains were resistant to aminoglycosides and/or fluoroquinolones. The VITEK2 system is highly reliable for methicillin resistance detection at the routine level. Oxacillin-susceptible classified clinical strains with associated resistance patterns required attention.

Authors+Show Affiliations

Service de Bactériologie-Hygiène, CHU de Nantes, Nantes, France. pascale.bemer@chu-nantes.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

20372955

Citation

Bemer, P, et al. "Correlation Between the VITEK2 System and Cefoxitin Disk Diffusion for the Daily Detection of Oxacillin Resistance in a Large Number of Clinical Staphylococcus Aureus Isolates." European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, vol. 29, no. 6, 2010, pp. 745-7.
Bemer P, Juvin ME, Le Gargasson G, et al. Correlation between the VITEK2 system and cefoxitin disk diffusion for the daily detection of oxacillin resistance in a large number of clinical Staphylococcus aureus isolates. Eur J Clin Microbiol Infect Dis. 2010;29(6):745-7.
Bemer, P., Juvin, M. E., Le Gargasson, G., Drugeon, H., Reynaud, A., & Corvec, S. (2010). Correlation between the VITEK2 system and cefoxitin disk diffusion for the daily detection of oxacillin resistance in a large number of clinical Staphylococcus aureus isolates. European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, 29(6), 745-7. https://doi.org/10.1007/s10096-010-0920-4
Bemer P, et al. Correlation Between the VITEK2 System and Cefoxitin Disk Diffusion for the Daily Detection of Oxacillin Resistance in a Large Number of Clinical Staphylococcus Aureus Isolates. Eur J Clin Microbiol Infect Dis. 2010;29(6):745-7. PubMed PMID: 20372955.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Correlation between the VITEK2 system and cefoxitin disk diffusion for the daily detection of oxacillin resistance in a large number of clinical Staphylococcus aureus isolates. AU - Bemer,P, AU - Juvin,M E, AU - Le Gargasson,G, AU - Drugeon,H, AU - Reynaud,A, AU - Corvec,S, Y1 - 2010/04/06/ PY - 2009/09/15/received PY - 2010/01/21/accepted PY - 2010/4/8/entrez PY - 2010/4/8/pubmed PY - 2010/8/10/medline SP - 745 EP - 7 JF - European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology JO - Eur. J. Clin. Microbiol. Infect. Dis. VL - 29 IS - 6 N2 - The aim of the present study was to compare the performance of the new VITEK2 AST-P551 card with the cefoxitin disk diffusion method for the daily detection of methicillin resistance with a high number of Staphylococcus aureus clinical isolates. Detection of the PBP2a protein or mecA gene was performed for each discordant case. Seventy (3.3%) isolates out of 2,107 clinical strains showed discordant results, two very major errors, four major errors and 64 minor errors. Fifty-nine (84%) discordant results were resolved, with a final overall agreement of 99.5%. Eleven (0.5%) strains remained discordant (minor error [mE]). Four of 370 MRSA strains were misclassified as susceptible in daily practice by the cefoxitin disk diffusion method. All of these strains were resistant to aminoglycosides and/or fluoroquinolones. The VITEK2 system is highly reliable for methicillin resistance detection at the routine level. Oxacillin-susceptible classified clinical strains with associated resistance patterns required attention. SN - 1435-4373 UR - https://www.unboundmedicine.com/medline/citation/20372955/Correlation_between_the_VITEK2_system_and_cefoxitin_disk_diffusion_for_the_daily_detection_of_oxacillin_resistance_in_a_large_number_of_clinical_Staphylococcus_aureus_isolates_ L2 - https://dx.doi.org/10.1007/s10096-010-0920-4 DB - PRIME DP - Unbound Medicine ER -