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Prevalence, characterization, and genotypic analysis of Escherichia coli O157:H7/NM from selected beef exporting abattoirs of Argentina.
J Food Prot. 2010 Apr; 73(4):649-56.JF

Abstract

In Argentina, Escherichia coli O157:H7/NM (STEC O157) is the prevalent serotype associated with hemolytic uremic syndrome (HUS), which is endemic in the country with more than 400 cases per year. In order to estimate the prevalence and characteristics of STEC O157 in beef cattle at slaughter, a survey of 1,622 fecal and carcass samples was conducted in nine beef exporting abattoirs from November 2006 to April 2008. A total of 54 samples were found positive for STEC O157, with an average prevalence of 4.1% in fecal content and 2.6% in carcasses. Calves and heifers presented higher percentages of prevalence in feces, 10.5 and 8.5%, respectively. All STEC O157 isolates harbored stx(2) (Shiga toxin 2), eae (intimin), ehxA (enterohemolysin), and fliC(H7) (H7 flagellin) genes, while stx(1) (Shiga toxin 1) was present in 16.7% of the strains. The prevalent (56%) stx genotype identified was stx(2) combined with variant stx(2c (vh-a)), the combination of which is also prevalent (>90%) in STEC O157 post-enteric HUS cases in Argentina. The clonal relatedness of STEC O157 strains was established by phage typing and pulsed-field gel electrophoresis (PFGE). The 54 STEC isolates were categorized into 12 different phage types and in 29 XbaI-PFGE patterns distributed in 27 different lots. STEC O157 strains isolated from 5 of 21 carcasses were identical by PFGE (100% similarity) to strains of the fecal content of the same or a contiguous bovine in the lot. Five phage type-PFGE-stx profiles of 10 strains isolated in this study matched with the profiles of the strains recovered from 18 of 122 HUS cases that occurred in the same period.

Authors+Show Affiliations

Instituto Tecnología de Alimentos, Centro de Investigación de Agroindustria, Instituto Nacional de Tecnología Agropecuaria, INTA, CC. 77, B1708WAB Morón, Pcia. de Buenos Aires, Argentina. mmasana@cnia.inta.gov.arNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20377952

Citation

Masana, M O., et al. "Prevalence, Characterization, and Genotypic Analysis of Escherichia Coli O157:H7/NM From Selected Beef Exporting Abattoirs of Argentina." Journal of Food Protection, vol. 73, no. 4, 2010, pp. 649-56.
Masana MO, Leotta GA, Del Castillo LL, et al. Prevalence, characterization, and genotypic analysis of Escherichia coli O157:H7/NM from selected beef exporting abattoirs of Argentina. J Food Prot. 2010;73(4):649-56.
Masana, M. O., Leotta, G. A., Del Castillo, L. L., D'Astek, B. A., Palladino, P. M., Galli, L., Vilacoba, E., Carbonari, C., Rodríguez, H. R., & Rivas, M. (2010). Prevalence, characterization, and genotypic analysis of Escherichia coli O157:H7/NM from selected beef exporting abattoirs of Argentina. Journal of Food Protection, 73(4), 649-56.
Masana MO, et al. Prevalence, Characterization, and Genotypic Analysis of Escherichia Coli O157:H7/NM From Selected Beef Exporting Abattoirs of Argentina. J Food Prot. 2010;73(4):649-56. PubMed PMID: 20377952.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence, characterization, and genotypic analysis of Escherichia coli O157:H7/NM from selected beef exporting abattoirs of Argentina. AU - Masana,M O, AU - Leotta,G A, AU - Del Castillo,L L, AU - D'Astek,B A, AU - Palladino,P M, AU - Galli,L, AU - Vilacoba,E, AU - Carbonari,C, AU - Rodríguez,H R, AU - Rivas,M, PY - 2010/4/10/entrez PY - 2010/4/10/pubmed PY - 2010/5/12/medline SP - 649 EP - 56 JF - Journal of food protection JO - J Food Prot VL - 73 IS - 4 N2 - In Argentina, Escherichia coli O157:H7/NM (STEC O157) is the prevalent serotype associated with hemolytic uremic syndrome (HUS), which is endemic in the country with more than 400 cases per year. In order to estimate the prevalence and characteristics of STEC O157 in beef cattle at slaughter, a survey of 1,622 fecal and carcass samples was conducted in nine beef exporting abattoirs from November 2006 to April 2008. A total of 54 samples were found positive for STEC O157, with an average prevalence of 4.1% in fecal content and 2.6% in carcasses. Calves and heifers presented higher percentages of prevalence in feces, 10.5 and 8.5%, respectively. All STEC O157 isolates harbored stx(2) (Shiga toxin 2), eae (intimin), ehxA (enterohemolysin), and fliC(H7) (H7 flagellin) genes, while stx(1) (Shiga toxin 1) was present in 16.7% of the strains. The prevalent (56%) stx genotype identified was stx(2) combined with variant stx(2c (vh-a)), the combination of which is also prevalent (>90%) in STEC O157 post-enteric HUS cases in Argentina. The clonal relatedness of STEC O157 strains was established by phage typing and pulsed-field gel electrophoresis (PFGE). The 54 STEC isolates were categorized into 12 different phage types and in 29 XbaI-PFGE patterns distributed in 27 different lots. STEC O157 strains isolated from 5 of 21 carcasses were identical by PFGE (100% similarity) to strains of the fecal content of the same or a contiguous bovine in the lot. Five phage type-PFGE-stx profiles of 10 strains isolated in this study matched with the profiles of the strains recovered from 18 of 122 HUS cases that occurred in the same period. SN - 0362-028X UR - https://www.unboundmedicine.com/medline/citation/20377952/Prevalence_characterization_and_genotypic_analysis_of_Escherichia_coli_O157:H7/NM_from_selected_beef_exporting_abattoirs_of_Argentina_ L2 - https://meridian.allenpress.com/jfp/article-lookup/doi/10.4315/0362-028x-73.4.649 DB - PRIME DP - Unbound Medicine ER -