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Impact of sulfur starvation on cysteine biosynthesis in T-DNA mutants deficient for compartment-specific serine-acetyltransferase.
Amino Acids 2010; 39(4):1029-42AA

Abstract

Sulfur plays a pivotal role in the cellular metabolism of many organisms. In plants, the uptake and assimilation of sulfate is strongly regulated at the transcriptional level. Regulatory factors are the demand of reduced sulfur in organic or non-organic form and the level of O-acetylserine (OAS), the carbon precursor for cysteine biosynthesis. In plants, cysteine is synthesized by action of the cysteine-synthase complex (CSC) containing serine acetyltransferase (SAT) and O-acetylserine-(thiol)-lyase (OASTL). Both enzymes are located in plastids, mitochondria and the cytosol. The function of the compartmentation of the CSC to regulate sulfate uptake and assimilation is still not clearly resolved. To address this question, we analyzed Arabidopsis thaliana mutants for the plastidic and cytosolic SAT isoenzymes under sulfur starvation conditions. In addition, subcellular metabolite analysis by non-aqueous fractionation revealed distinct changes in subcellular metabolite distribution upon short-term sulfur starvation. Metabolite and transcript analyses of SERAT1.1 and SERAT2.1 mutants [previously analyzed in Krueger et al. (Plant Cell Environ 32:349-367, 2009)] grown under sulfur starvation conditions indicate that both isoenzymes do not contribute directly to the transcriptional regulation of genes involved in sulfate uptake and assimilation. Here, we summarize the current knowledge about the regulation of cysteine biosynthesis and the contribution of the different compartments to this metabolic process. We relate hypotheses and views of the regulation of cysteine biosynthesis with our results of applying sulfur starvation to mutants impaired in compartment-specific cysteine biosynthetic enzymes.

Authors+Show Affiliations

Max Planck Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20379751

Citation

Krueger, Stephan, et al. "Impact of Sulfur Starvation On Cysteine Biosynthesis in T-DNA Mutants Deficient for Compartment-specific Serine-acetyltransferase." Amino Acids, vol. 39, no. 4, 2010, pp. 1029-42.
Krueger S, Donath A, Lopez-Martin MC, et al. Impact of sulfur starvation on cysteine biosynthesis in T-DNA mutants deficient for compartment-specific serine-acetyltransferase. Amino Acids. 2010;39(4):1029-42.
Krueger, S., Donath, A., Lopez-Martin, M. C., Hoefgen, R., Gotor, C., & Hesse, H. (2010). Impact of sulfur starvation on cysteine biosynthesis in T-DNA mutants deficient for compartment-specific serine-acetyltransferase. Amino Acids, 39(4), pp. 1029-42. doi:10.1007/s00726-010-0580-9.
Krueger S, et al. Impact of Sulfur Starvation On Cysteine Biosynthesis in T-DNA Mutants Deficient for Compartment-specific Serine-acetyltransferase. Amino Acids. 2010;39(4):1029-42. PubMed PMID: 20379751.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impact of sulfur starvation on cysteine biosynthesis in T-DNA mutants deficient for compartment-specific serine-acetyltransferase. AU - Krueger,Stephan, AU - Donath,Andrea, AU - Lopez-Martin,M Carmen, AU - Hoefgen,Rainer, AU - Gotor,Cecilia, AU - Hesse,Holger, Y1 - 2010/04/09/ PY - 2010/01/19/received PY - 2010/03/22/accepted PY - 2010/4/10/entrez PY - 2010/4/10/pubmed PY - 2010/12/14/medline SP - 1029 EP - 42 JF - Amino acids JO - Amino Acids VL - 39 IS - 4 N2 - Sulfur plays a pivotal role in the cellular metabolism of many organisms. In plants, the uptake and assimilation of sulfate is strongly regulated at the transcriptional level. Regulatory factors are the demand of reduced sulfur in organic or non-organic form and the level of O-acetylserine (OAS), the carbon precursor for cysteine biosynthesis. In plants, cysteine is synthesized by action of the cysteine-synthase complex (CSC) containing serine acetyltransferase (SAT) and O-acetylserine-(thiol)-lyase (OASTL). Both enzymes are located in plastids, mitochondria and the cytosol. The function of the compartmentation of the CSC to regulate sulfate uptake and assimilation is still not clearly resolved. To address this question, we analyzed Arabidopsis thaliana mutants for the plastidic and cytosolic SAT isoenzymes under sulfur starvation conditions. In addition, subcellular metabolite analysis by non-aqueous fractionation revealed distinct changes in subcellular metabolite distribution upon short-term sulfur starvation. Metabolite and transcript analyses of SERAT1.1 and SERAT2.1 mutants [previously analyzed in Krueger et al. (Plant Cell Environ 32:349-367, 2009)] grown under sulfur starvation conditions indicate that both isoenzymes do not contribute directly to the transcriptional regulation of genes involved in sulfate uptake and assimilation. Here, we summarize the current knowledge about the regulation of cysteine biosynthesis and the contribution of the different compartments to this metabolic process. We relate hypotheses and views of the regulation of cysteine biosynthesis with our results of applying sulfur starvation to mutants impaired in compartment-specific cysteine biosynthetic enzymes. SN - 1438-2199 UR - https://www.unboundmedicine.com/medline/citation/20379751/Impact_of_sulfur_starvation_on_cysteine_biosynthesis_in_T_DNA_mutants_deficient_for_compartment_specific_serine_acetyltransferase_ L2 - https://dx.doi.org/10.1007/s00726-010-0580-9 DB - PRIME DP - Unbound Medicine ER -