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The assessment of bee venom responses in an experimental model of mono-arthritis using Tc-99m DPD bone scintigraphy.

Abstract

OBJECTIVES

Several recent studies have shown that bee venom (BV) has an anti-nociceptive and anti-inflammatory effect on arthritis. However, objective methods for evaluation of the therapeutic effect of BV is insufficient in animal studies and clinical trials. Our purpose was to determine the usefulness of bone scintigraphy using Tc-99m DPD (3,3-diphosphono-1,2-propan-dicarbonacid) about effects of BV applied to carrageenan-induced mono-arthritis (CIA) model.

METHODS

Mono-arthritis was induced by an intra-articular injection of carrageenan in Sprague-Dawley rats. Administration of BV (0.8 mg/kg) was performed at 30 min before and at 4 h after the induction of mono-arthritis. We assigned rats to BV-before, BV-after, control-before and control-after groups and compared the results of each group by the weight-loading test and bone scintigraphy. The rats received an intravenous injection of 37 MBq of Tc-99m DPD by the tail vein and then scanning was performed at 4 and 24 h after the injection. Visual assessment and quantitative analysis were performed for both knees.

RESULTS

The BV-before and BV-after groups were more improved than the control groups on the weight load test (p < 0.05). Bone scintigraphy showed lower activity in the BV-before group than in the control-before group (p < 0.05) on the 4 h imaging. However, a significant difference in the BV-before and BV-after groups was not observed on the 24 h imaging.

CONCLUSIONS

BV had therapeutic effects by anti-nociceptive and anti-inflammatory activity in the CIA and bone scintigraphy performed on 4 h imaging provided visual and quantitative information for the assessment of the therapeutic response to BV as an objective method in mono-arthritis model.

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  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of Physical Medicine and Rehabilitation, College of Medicine, Wonkwang University, Iksan, Republic of Korea.

    , ,

    Source

    Annals of nuclear medicine 24:6 2010 Jul pg 455-60

    MeSH

    Acupuncture Therapy
    Animals
    Arthritis
    Bee Venoms
    Behavior, Animal
    Bone and Bones
    Carrageenan
    Diphosphonates
    Disease Models, Animal
    Knee Joint
    Male
    Organotechnetium Compounds
    Rats
    Rats, Sprague-Dawley
    Treatment Outcome

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    20379856

    Citation

    TY - JOUR T1 - The assessment of bee venom responses in an experimental model of mono-arthritis using Tc-99m DPD bone scintigraphy. AU - Yang,Chung-Yong, AU - Park,Soon-Ah, AU - Oh,Kyung-Jae, AU - Yang,Yun Sik, Y1 - 2010/04/09/ PY - 2010/1/18/received PY - 2010/3/20/accepted PY - 2010/4/9/aheadofprint PY - 2010/4/10/entrez PY - 2010/4/10/pubmed PY - 2010/11/4/medline SP - 455 EP - 60 JF - Annals of nuclear medicine JO - Ann Nucl Med VL - 24 IS - 6 N2 - OBJECTIVES: Several recent studies have shown that bee venom (BV) has an anti-nociceptive and anti-inflammatory effect on arthritis. However, objective methods for evaluation of the therapeutic effect of BV is insufficient in animal studies and clinical trials. Our purpose was to determine the usefulness of bone scintigraphy using Tc-99m DPD (3,3-diphosphono-1,2-propan-dicarbonacid) about effects of BV applied to carrageenan-induced mono-arthritis (CIA) model. METHODS: Mono-arthritis was induced by an intra-articular injection of carrageenan in Sprague-Dawley rats. Administration of BV (0.8 mg/kg) was performed at 30 min before and at 4 h after the induction of mono-arthritis. We assigned rats to BV-before, BV-after, control-before and control-after groups and compared the results of each group by the weight-loading test and bone scintigraphy. The rats received an intravenous injection of 37 MBq of Tc-99m DPD by the tail vein and then scanning was performed at 4 and 24 h after the injection. Visual assessment and quantitative analysis were performed for both knees. RESULTS: The BV-before and BV-after groups were more improved than the control groups on the weight load test (p < 0.05). Bone scintigraphy showed lower activity in the BV-before group than in the control-before group (p < 0.05) on the 4 h imaging. However, a significant difference in the BV-before and BV-after groups was not observed on the 24 h imaging. CONCLUSIONS: BV had therapeutic effects by anti-nociceptive and anti-inflammatory activity in the CIA and bone scintigraphy performed on 4 h imaging provided visual and quantitative information for the assessment of the therapeutic response to BV as an objective method in mono-arthritis model. SN - 1864-6433 UR - https://www.unboundmedicine.com/medline/citation/20379856/full_citation/The_assessment_of_bee_venom_responses_in_an_experimental_model_of_mono_arthritis_using_Tc_99m_DPD_bone_scintigraphy_ L2 - http://dx.doi.org/10.1007/s12149-010-0380-z ER -