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Time-dependant protective effects of mangenese(III) tetrakis (1-methyl-4-pyridyl) porphyrin on mitochondrial function following renal ischemia-reperfusion injury.
Free Radic Res. 2010 Jul; 44(7):773-82.FR

Abstract

This study examined the time-dependent effects of a cell permeable SOD mimetic, MnTMPyP, on mitochondrial function in renal ischemia-reperfusion injury (IRI). Male SD rats were subject to either sham operation or bilateral renal ischemia for 45 min followed by reperfusion for 1, 4 or 24 h. A sub-set of animals was treated with either saline vehicle or 5 mg/Kg of MnTMPyP (i.p.). EPR measurements showed that at 1-h reperfusion MnTMPyP prevented a decrease in aconitase activity (p < 0.05) and attenuated the increase in the high spin heme at g = 6 and oxidation of 4Fe4S to 3Fe4S signal at g = 2.015 (p < 0.01). MnTMPyP was effective in preventing loss of mitochondrial complexes and prevented the loss of cytochrome c and Smac/Diablo from mitochondria early in reperfusion. Following 24 h of reperfusion MnTMPyP was effective in attenuating caspase-3 and blocking apoptosis (p < 0.05). In conclusion, MnTMPyP has biphasic effects in renal IRI, inhibiting mitochondrial dysfunction at the early phases of reperfusion and prevention of apoptosis following longer durations of reperfusion.

Authors+Show Affiliations

Kidney Disease Center, Medical College of Wisconsin, 53226, USA. vnilakan@mcw.edu <vnilakan@mcw.edu>No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20380592

Citation

Nilakantan, Vani, et al. "Time-dependant Protective Effects of mangenese(III) Tetrakis (1-methyl-4-pyridyl) Porphyrin On Mitochondrial Function Following Renal Ischemia-reperfusion Injury." Free Radical Research, vol. 44, no. 7, 2010, pp. 773-82.
Nilakantan V, Liang HL, Rajesh S, et al. Time-dependant protective effects of mangenese(III) tetrakis (1-methyl-4-pyridyl) porphyrin on mitochondrial function following renal ischemia-reperfusion injury. Free Radic Res. 2010;44(7):773-82.
Nilakantan, V., Liang, H. L., Rajesh, S., Mortensen, J., & Chandran, K. (2010). Time-dependant protective effects of mangenese(III) tetrakis (1-methyl-4-pyridyl) porphyrin on mitochondrial function following renal ischemia-reperfusion injury. Free Radical Research, 44(7), 773-82. https://doi.org/10.3109/10715761003786164
Nilakantan V, et al. Time-dependant Protective Effects of mangenese(III) Tetrakis (1-methyl-4-pyridyl) Porphyrin On Mitochondrial Function Following Renal Ischemia-reperfusion Injury. Free Radic Res. 2010;44(7):773-82. PubMed PMID: 20380592.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Time-dependant protective effects of mangenese(III) tetrakis (1-methyl-4-pyridyl) porphyrin on mitochondrial function following renal ischemia-reperfusion injury. AU - Nilakantan,Vani, AU - Liang,Huan Ling, AU - Rajesh,Seenivasan, AU - Mortensen,Jordan, AU - Chandran,Karunakaran, PY - 2010/4/13/entrez PY - 2010/4/13/pubmed PY - 2010/10/13/medline SP - 773 EP - 82 JF - Free radical research JO - Free Radic Res VL - 44 IS - 7 N2 - This study examined the time-dependent effects of a cell permeable SOD mimetic, MnTMPyP, on mitochondrial function in renal ischemia-reperfusion injury (IRI). Male SD rats were subject to either sham operation or bilateral renal ischemia for 45 min followed by reperfusion for 1, 4 or 24 h. A sub-set of animals was treated with either saline vehicle or 5 mg/Kg of MnTMPyP (i.p.). EPR measurements showed that at 1-h reperfusion MnTMPyP prevented a decrease in aconitase activity (p < 0.05) and attenuated the increase in the high spin heme at g = 6 and oxidation of 4Fe4S to 3Fe4S signal at g = 2.015 (p < 0.01). MnTMPyP was effective in preventing loss of mitochondrial complexes and prevented the loss of cytochrome c and Smac/Diablo from mitochondria early in reperfusion. Following 24 h of reperfusion MnTMPyP was effective in attenuating caspase-3 and blocking apoptosis (p < 0.05). In conclusion, MnTMPyP has biphasic effects in renal IRI, inhibiting mitochondrial dysfunction at the early phases of reperfusion and prevention of apoptosis following longer durations of reperfusion. SN - 1029-2470 UR - https://www.unboundmedicine.com/medline/citation/20380592/Time_dependant_protective_effects_of_mangenese_III__tetrakis__1_methyl_4_pyridyl__porphyrin_on_mitochondrial_function_following_renal_ischemia_reperfusion_injury_ L2 - https://www.tandfonline.com/doi/full/10.3109/10715761003786164 DB - PRIME DP - Unbound Medicine ER -