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Determination of malachite green residues in fish using molecularly imprinted solid-phase extraction followed by liquid chromatography-linear ion trap mass spectrometry.
Anal Chim Acta. 2010 Apr 14; 665(1):47-54.AC

Abstract

This paper describes the development of an analytical procedure to determine malachite green (MG) residues in salmon samples using molecularly imprinted polymers (MIPs) as the extraction and clean-up material, followed by liquid chromatography-linear ion trap mass spectrometry (LC-QqQLIT-MS/MS). MG and two structurally related compounds, crystal violet (CV) and brilliant green (BG) were employed for the selectivity test. The imprinted polymers exhibited high binding affinity for MG, while CV and BG showed less binding capacity: 47% and 34%, respectively. The recovery values of MG in salmon samples fortified with leucomalachite green (LMG) were determined by measuring the amount of MG in the sample, after carrying out the oxidation reaction with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), which converts the LMG back into chromic-form. The average recovery of MG in spiked salmon muscle over the concentration range 1-100 ng g(-1) was 98% with a relative standard deviation value (R.S.D.) below 12%. The method detection limits (MDLs) obtained for MG, CV, BG and their leuco-metabolites were in the range of 3-20 ng kg(-1) (ppt).

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Authors+Show Affiliations

Martínez Bueno MJ
Pesticide Residues Research Group, Department of Hydrogeology and Analytical Chemistry, University of Almería, 04120 La Cañada de San Urbano, Almería, Spain.
Herrera S
No affiliation info available
Uclés A
No affiliation info available
Agüera A
No affiliation info available
Hernando MD
No affiliation info available
Shimelis O
No affiliation info available
Rudolfsson M
No affiliation info available
Fernández-Alba AR
No affiliation info available

MeSH

AnimalsChromatography, High Pressure LiquidGentian VioletMolecular ImprintingQuaternary Ammonium CompoundsRosaniline DyesSalmonSolid Phase ExtractionSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20381689

Citation

Martínez Bueno, María Jesús, et al. "Determination of Malachite Green Residues in Fish Using Molecularly Imprinted Solid-phase Extraction Followed By Liquid Chromatography-linear Ion Trap Mass Spectrometry." Analytica Chimica Acta, vol. 665, no. 1, 2010, pp. 47-54.
Martínez Bueno MJ, Herrera S, Uclés A, et al. Determination of malachite green residues in fish using molecularly imprinted solid-phase extraction followed by liquid chromatography-linear ion trap mass spectrometry. Anal Chim Acta. 2010;665(1):47-54.
Martínez Bueno, M. J., Herrera, S., Uclés, A., Agüera, A., Hernando, M. D., Shimelis, O., Rudolfsson, M., & Fernández-Alba, A. R. (2010). Determination of malachite green residues in fish using molecularly imprinted solid-phase extraction followed by liquid chromatography-linear ion trap mass spectrometry. Analytica Chimica Acta, 665(1), 47-54. https://doi.org/10.1016/j.aca.2010.03.001
Martínez Bueno MJ, et al. Determination of Malachite Green Residues in Fish Using Molecularly Imprinted Solid-phase Extraction Followed By Liquid Chromatography-linear Ion Trap Mass Spectrometry. Anal Chim Acta. 2010 Apr 14;665(1):47-54. PubMed PMID: 20381689.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of malachite green residues in fish using molecularly imprinted solid-phase extraction followed by liquid chromatography-linear ion trap mass spectrometry. AU - Martínez Bueno,María Jesús, AU - Herrera,Sonia, AU - Uclés,Ana, AU - Agüera,Ana, AU - Hernando,María Dolores, AU - Shimelis,Olga, AU - Rudolfsson,Marcus, AU - Fernández-Alba,Amadeo R, Y1 - 2010/03/06/ PY - 2009/12/14/received PY - 2010/02/02/revised PY - 2010/03/01/accepted PY - 2010/4/13/entrez PY - 2010/4/13/pubmed PY - 2010/6/30/medline SP - 47 EP - 54 JF - Analytica chimica acta JO - Anal Chim Acta VL - 665 IS - 1 N2 - This paper describes the development of an analytical procedure to determine malachite green (MG) residues in salmon samples using molecularly imprinted polymers (MIPs) as the extraction and clean-up material, followed by liquid chromatography-linear ion trap mass spectrometry (LC-QqQLIT-MS/MS). MG and two structurally related compounds, crystal violet (CV) and brilliant green (BG) were employed for the selectivity test. The imprinted polymers exhibited high binding affinity for MG, while CV and BG showed less binding capacity: 47% and 34%, respectively. The recovery values of MG in salmon samples fortified with leucomalachite green (LMG) were determined by measuring the amount of MG in the sample, after carrying out the oxidation reaction with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), which converts the LMG back into chromic-form. The average recovery of MG in spiked salmon muscle over the concentration range 1-100 ng g(-1) was 98% with a relative standard deviation value (R.S.D.) below 12%. The method detection limits (MDLs) obtained for MG, CV, BG and their leuco-metabolites were in the range of 3-20 ng kg(-1) (ppt). SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/20381689/Determination_of_malachite_green_residues_in_fish_using_molecularly_imprinted_solid_phase_extraction_followed_by_liquid_chromatography_linear_ion_trap_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(10)00284-9 DB - PRIME DP - Unbound Medicine ER -
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