Tags

Type your tag names separated by a space and hit enter

Higher CD34(+) and CD3(+) cell doses in the graft promote long-term survival, and have no impact on the incidence of severe acute or chronic graft-versus-host disease after in vivo T cell-depleted unrelated donor hematopoietic stem cell transplantation in children.
Biol Blood Marrow Transplant. 2010 Oct; 16(10):1388-401.BB

Abstract

The aim of our study was to compare the results of unrelated donor (UD) peripheral blood stem cell transplantation versus UD bone marrow transplantation and to analyze the impact of infused CD34(+) and CD3(+) cell doses on survival and incidence of severe graft-versus-host disease (GVHD) in 187 children who underwent UD hematopoietic cell transplantation with the use of in vivo T cell depletion (antithymocyte globulin or CAMPATH-1H). HLA typing was performed at the "high-resolution" level. Patients receiving > or =10 x 10(6) CD34(+) cells/kg and > or =4 x 10(8) CD3(+) cells/kg had better overall and disease-free survival. Multivariate analysis has shown that both infused CD34(+) cell dose <10 x 10(6)/kg and CD3(+) cell dose <4 x 10(8)/kg were independent risk factors for mortality (relative risk [RR] 1.8 and 1.71, P = .009 and .016, respectively). Regarding disease-free survival, multivariate analysis has revealed another independent risk factor for poor outcome apart from the 2 earlier-mentioned cell doses, which was the use of donors mismatched at 2 HLA antigens or 3 HLA allele/antigens (RR 2.5, P = .004). In age groups 0-10 years and 10-20 years, CD34(+) cell doses higher than the age-adjusted median dose clearly favored survival. Higher infused doses of CD34(+) and CD3(+) cells did not result in an increased rate of severe GVHD. The use of mismatched donors was the only independent risk factor for the incidence of severe acute GVHD (RR 2.2, P = .046). The report demonstrates for the first time in a pediatric cohort, that higher doses of transplanted CD34(+) and CD3(+) cells lead to an improved survival without an increased risk of severe GVHD. The study findings may be limited to the population of patients receiving in vivo T cell depletion, which is now broadly used in unrelated donor setting in Europe.

Authors+Show Affiliations

Department of Pediatric Hematology, Oncology and Bone Marrow Transplantation, Wroclaw Medical University, Poland. kk@pedhemat.am.wroc.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20382248

Citation

Kałwak, Krzysztof, et al. "Higher CD34(+) and CD3(+) Cell Doses in the Graft Promote Long-term Survival, and Have No Impact On the Incidence of Severe Acute or Chronic Graft-versus-host Disease After in Vivo T Cell-depleted Unrelated Donor Hematopoietic Stem Cell Transplantation in Children." Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation, vol. 16, no. 10, 2010, pp. 1388-401.
Kałwak K, Porwolik J, Mielcarek M, et al. Higher CD34(+) and CD3(+) cell doses in the graft promote long-term survival, and have no impact on the incidence of severe acute or chronic graft-versus-host disease after in vivo T cell-depleted unrelated donor hematopoietic stem cell transplantation in children. Biol Blood Marrow Transplant. 2010;16(10):1388-401.
Kałwak, K., Porwolik, J., Mielcarek, M., Gorczyńska, E., Owoc-Lempach, J., Ussowicz, M., Dyla, A., Musiał, J., Paździor, D., Turkiewicz, D., & Chybicka, A. (2010). Higher CD34(+) and CD3(+) cell doses in the graft promote long-term survival, and have no impact on the incidence of severe acute or chronic graft-versus-host disease after in vivo T cell-depleted unrelated donor hematopoietic stem cell transplantation in children. Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation, 16(10), 1388-401. https://doi.org/10.1016/j.bbmt.2010.04.001
Kałwak K, et al. Higher CD34(+) and CD3(+) Cell Doses in the Graft Promote Long-term Survival, and Have No Impact On the Incidence of Severe Acute or Chronic Graft-versus-host Disease After in Vivo T Cell-depleted Unrelated Donor Hematopoietic Stem Cell Transplantation in Children. Biol Blood Marrow Transplant. 2010;16(10):1388-401. PubMed PMID: 20382248.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Higher CD34(+) and CD3(+) cell doses in the graft promote long-term survival, and have no impact on the incidence of severe acute or chronic graft-versus-host disease after in vivo T cell-depleted unrelated donor hematopoietic stem cell transplantation in children. AU - Kałwak,Krzysztof, AU - Porwolik,Julita, AU - Mielcarek,Monika, AU - Gorczyńska,Ewa, AU - Owoc-Lempach,Joanna, AU - Ussowicz,Marek, AU - Dyla,Agnieszka, AU - Musiał,Jakub, AU - Paździor,Dominika, AU - Turkiewicz,Dominik, AU - Chybicka,Alicja, Y1 - 2010/04/09/ PY - 2009/12/14/received PY - 2010/04/01/accepted PY - 2010/4/13/entrez PY - 2010/4/13/pubmed PY - 2011/2/1/medline SP - 1388 EP - 401 JF - Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation JO - Biol. Blood Marrow Transplant. VL - 16 IS - 10 N2 - The aim of our study was to compare the results of unrelated donor (UD) peripheral blood stem cell transplantation versus UD bone marrow transplantation and to analyze the impact of infused CD34(+) and CD3(+) cell doses on survival and incidence of severe graft-versus-host disease (GVHD) in 187 children who underwent UD hematopoietic cell transplantation with the use of in vivo T cell depletion (antithymocyte globulin or CAMPATH-1H). HLA typing was performed at the "high-resolution" level. Patients receiving > or =10 x 10(6) CD34(+) cells/kg and > or =4 x 10(8) CD3(+) cells/kg had better overall and disease-free survival. Multivariate analysis has shown that both infused CD34(+) cell dose <10 x 10(6)/kg and CD3(+) cell dose <4 x 10(8)/kg were independent risk factors for mortality (relative risk [RR] 1.8 and 1.71, P = .009 and .016, respectively). Regarding disease-free survival, multivariate analysis has revealed another independent risk factor for poor outcome apart from the 2 earlier-mentioned cell doses, which was the use of donors mismatched at 2 HLA antigens or 3 HLA allele/antigens (RR 2.5, P = .004). In age groups 0-10 years and 10-20 years, CD34(+) cell doses higher than the age-adjusted median dose clearly favored survival. Higher infused doses of CD34(+) and CD3(+) cells did not result in an increased rate of severe GVHD. The use of mismatched donors was the only independent risk factor for the incidence of severe acute GVHD (RR 2.2, P = .046). The report demonstrates for the first time in a pediatric cohort, that higher doses of transplanted CD34(+) and CD3(+) cells lead to an improved survival without an increased risk of severe GVHD. The study findings may be limited to the population of patients receiving in vivo T cell depletion, which is now broadly used in unrelated donor setting in Europe. SN - 1523-6536 UR - https://www.unboundmedicine.com/medline/citation/20382248/Higher_CD34_+__and_CD3_+__cell_doses_in_the_graft_promote_long_term_survival_and_have_no_impact_on_the_incidence_of_severe_acute_or_chronic_graft_versus_host_disease_after_in_vivo_T_cell_depleted_unrelated_donor_hematopoietic_stem_cell_transplantation_in_children_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1083-8791(10)00148-5 DB - PRIME DP - Unbound Medicine ER -