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Cytotoxic and mutagenic evaluation of extracts from plant species of the Miconia genus and their influence on doxorubicin-induced mutagenicity: an in vitro analysis.
Exp Toxicol Pathol. 2011 Jul; 63(5):499-504.ET

Abstract

The Miconia genus, a plant widely used for medicine, occurs in tropical America and its extracts and isolated compounds have demonstrated antibiotic, antitumoral, analgesic and antimalarial activities. However, no study concerning its genotoxicity has been conducted and it is necessary to determine its potential mutagenic effects to develop products and chemicals from these extracts. This study assessed the cytotoxicity, mutagenicity and the protective effects of methanolic extracts from Miconia species on Chinese hamster lung fibroblast cell cultures (V79). The cytotoxicity was evaluated using a clonogenic assay. Cultures exposed to the extract of Miconia albicans up to a concentration of 30 μg/mL, M. cabucu up to 40 μg/mL, M. albicans up to 40 μg/mL and M. stenostachya up to 60 μg/mL exhibited a cytotoxic effect on the cells. The clonogenic assay used three non-cytotoxic concentrations (5, 10 and 20 μg/mL) to evaluate mutagenicity and antimutagenicity of the extracts. Cultures were treated with these three extract concentrations (mutagenicity test) or the extract associated with doxorubicin (DXR) (antimutagenicity test) in three protocols (pre-, simultaneous and post-treatments). Distilled water and DXR were used as negative and positive controls, respectively. In the micronucleus (MN) test, a significant reduction was observed in MN frequency in cultures treated with DXR and extracts compared to those receiving only DXR; a significant reduction was also observed for the presence of mutagenicity in all treatments. This study confirmed the safe use of Miconia extracts at the concentrations tested and reinforced the therapeutic properties previously described for Miconia species by showing their protective effects on doxorubicin-induced mutagenicity.

Authors+Show Affiliations

Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brasil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20385475

Citation

Mara Serpeloni, Juliana, et al. "Cytotoxic and Mutagenic Evaluation of Extracts From Plant Species of the Miconia Genus and Their Influence On Doxorubicin-induced Mutagenicity: an in Vitro Analysis." Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Fur Toxikologische Pathologie, vol. 63, no. 5, 2011, pp. 499-504.
Mara Serpeloni J, Mazzaron Barcelos GR, Prates Mori M, et al. Cytotoxic and mutagenic evaluation of extracts from plant species of the Miconia genus and their influence on doxorubicin-induced mutagenicity: an in vitro analysis. Exp Toxicol Pathol. 2011;63(5):499-504.
Mara Serpeloni, J., Mazzaron Barcelos, G. R., Prates Mori, M., Yanagui, K., Vilegas, W., Aparecida Varanda, E., & de Syllos Cólus, I. M. (2011). Cytotoxic and mutagenic evaluation of extracts from plant species of the Miconia genus and their influence on doxorubicin-induced mutagenicity: an in vitro analysis. Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Fur Toxikologische Pathologie, 63(5), 499-504. https://doi.org/10.1016/j.etp.2010.03.011
Mara Serpeloni J, et al. Cytotoxic and Mutagenic Evaluation of Extracts From Plant Species of the Miconia Genus and Their Influence On Doxorubicin-induced Mutagenicity: an in Vitro Analysis. Exp Toxicol Pathol. 2011;63(5):499-504. PubMed PMID: 20385475.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytotoxic and mutagenic evaluation of extracts from plant species of the Miconia genus and their influence on doxorubicin-induced mutagenicity: an in vitro analysis. AU - Mara Serpeloni,Juliana, AU - Mazzaron Barcelos,Gustavo Rafael, AU - Prates Mori,Mateus, AU - Yanagui,Karina, AU - Vilegas,Wagner, AU - Aparecida Varanda,Eliana, AU - de Syllos Cólus,Ilce Mara, Y1 - 2010/04/10/ PY - 2009/07/27/received PY - 2010/03/22/revised PY - 2010/03/25/accepted PY - 2010/4/14/entrez PY - 2010/4/14/pubmed PY - 2011/9/17/medline SP - 499 EP - 504 JF - Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie JO - Exp. Toxicol. Pathol. VL - 63 IS - 5 N2 - The Miconia genus, a plant widely used for medicine, occurs in tropical America and its extracts and isolated compounds have demonstrated antibiotic, antitumoral, analgesic and antimalarial activities. However, no study concerning its genotoxicity has been conducted and it is necessary to determine its potential mutagenic effects to develop products and chemicals from these extracts. This study assessed the cytotoxicity, mutagenicity and the protective effects of methanolic extracts from Miconia species on Chinese hamster lung fibroblast cell cultures (V79). The cytotoxicity was evaluated using a clonogenic assay. Cultures exposed to the extract of Miconia albicans up to a concentration of 30 μg/mL, M. cabucu up to 40 μg/mL, M. albicans up to 40 μg/mL and M. stenostachya up to 60 μg/mL exhibited a cytotoxic effect on the cells. The clonogenic assay used three non-cytotoxic concentrations (5, 10 and 20 μg/mL) to evaluate mutagenicity and antimutagenicity of the extracts. Cultures were treated with these three extract concentrations (mutagenicity test) or the extract associated with doxorubicin (DXR) (antimutagenicity test) in three protocols (pre-, simultaneous and post-treatments). Distilled water and DXR were used as negative and positive controls, respectively. In the micronucleus (MN) test, a significant reduction was observed in MN frequency in cultures treated with DXR and extracts compared to those receiving only DXR; a significant reduction was also observed for the presence of mutagenicity in all treatments. This study confirmed the safe use of Miconia extracts at the concentrations tested and reinforced the therapeutic properties previously described for Miconia species by showing their protective effects on doxorubicin-induced mutagenicity. SN - 1618-1433 UR - https://www.unboundmedicine.com/medline/citation/20385475/Cytotoxic_and_mutagenic_evaluation_of_extracts_from_plant_species_of_the_Miconia_genus_and_their_influence_on_doxorubicin_induced_mutagenicity:_an_in_vitro_analysis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0940-2993(10)00046-1 DB - PRIME DP - Unbound Medicine ER -