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Inhibition of auxin transport from the ovary or from the apical shoot induces parthenocarpic fruit-set in tomato mediated by gibberellins.
Plant Physiol. 2010 Jun; 153(2):851-62.PP

Abstract

Fruit-set in tomato (Solanum lycopersicum) depends on gibberellins and auxins (GAs). Here, we show, using the cv MicroTom, that application of N-1-naphthylphthalamic acid (NPA; an inhibitor of auxin transport) to unpollinated ovaries induced parthenocarpic fruit-set, associated with an increase of indole-3-acetic acid (IAA) content, and that this effect was negated by paclobutrazol (an inhibitor of GA biosynthesis). NPA-induced ovaries contained higher content of GA(1) (an active GA) and transcripts of GA biosynthetic genes (SlCPS, SlGA20ox1, and -2). Interestingly, application of NPA to pollinated ovaries prevented their growth, potentially due to supraoptimal IAA accumulation. Plant decapitation and inhibition of auxin transport by NPA from the apical shoot also induced parthenocarpic fruit growth of unpollinated ovaries. Application of IAA to the severed stump negated the plant decapitation effect, indicating that the apical shoot prevents unpollinated ovary growth through IAA transport. Parthenocarpic fruit growth induced by plant decapitation was associated with high levels of GA(1) and was counteracted by paclobutrazol treatment. Plant decapitation also produced changes in transcript levels of genes encoding enzymes of GA biosynthesis (SlCPS and SlGA20ox1) in the ovary, quite similar to those found in NPA-induced fruits. All these results suggest that auxin can have opposing effects on fruit-set, either inducing (when accumulated in the ovary) or repressing (when transported from the apical shoot) that process, and that GAs act as mediators in both cases. The effect of NPA application and decapitation on fruit-set induction was also observed in MicroTom lines bearing introgressed DWARF and SELF-PRUNING wild-type alleles.

Authors+Show Affiliations

Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, 46022 Valencia, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20388661

Citation

Serrani, Juan Carlos, et al. "Inhibition of Auxin Transport From the Ovary or From the Apical Shoot Induces Parthenocarpic Fruit-set in Tomato Mediated By Gibberellins." Plant Physiology, vol. 153, no. 2, 2010, pp. 851-62.
Serrani JC, Carrera E, Ruiz-Rivero O, et al. Inhibition of auxin transport from the ovary or from the apical shoot induces parthenocarpic fruit-set in tomato mediated by gibberellins. Plant Physiol. 2010;153(2):851-62.
Serrani, J. C., Carrera, E., Ruiz-Rivero, O., Gallego-Giraldo, L., Peres, L. E., & García-Martínez, J. L. (2010). Inhibition of auxin transport from the ovary or from the apical shoot induces parthenocarpic fruit-set in tomato mediated by gibberellins. Plant Physiology, 153(2), 851-62. https://doi.org/10.1104/pp.110.155424
Serrani JC, et al. Inhibition of Auxin Transport From the Ovary or From the Apical Shoot Induces Parthenocarpic Fruit-set in Tomato Mediated By Gibberellins. Plant Physiol. 2010;153(2):851-62. PubMed PMID: 20388661.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of auxin transport from the ovary or from the apical shoot induces parthenocarpic fruit-set in tomato mediated by gibberellins. AU - Serrani,Juan Carlos, AU - Carrera,Esther, AU - Ruiz-Rivero,Omar, AU - Gallego-Giraldo,Lina, AU - Peres,Lázaro Eustáquio Pereira, AU - García-Martínez,José Luis, Y1 - 2010/04/13/ PY - 2010/4/15/entrez PY - 2010/4/15/pubmed PY - 2010/9/16/medline SP - 851 EP - 62 JF - Plant physiology JO - Plant Physiol VL - 153 IS - 2 N2 - Fruit-set in tomato (Solanum lycopersicum) depends on gibberellins and auxins (GAs). Here, we show, using the cv MicroTom, that application of N-1-naphthylphthalamic acid (NPA; an inhibitor of auxin transport) to unpollinated ovaries induced parthenocarpic fruit-set, associated with an increase of indole-3-acetic acid (IAA) content, and that this effect was negated by paclobutrazol (an inhibitor of GA biosynthesis). NPA-induced ovaries contained higher content of GA(1) (an active GA) and transcripts of GA biosynthetic genes (SlCPS, SlGA20ox1, and -2). Interestingly, application of NPA to pollinated ovaries prevented their growth, potentially due to supraoptimal IAA accumulation. Plant decapitation and inhibition of auxin transport by NPA from the apical shoot also induced parthenocarpic fruit growth of unpollinated ovaries. Application of IAA to the severed stump negated the plant decapitation effect, indicating that the apical shoot prevents unpollinated ovary growth through IAA transport. Parthenocarpic fruit growth induced by plant decapitation was associated with high levels of GA(1) and was counteracted by paclobutrazol treatment. Plant decapitation also produced changes in transcript levels of genes encoding enzymes of GA biosynthesis (SlCPS and SlGA20ox1) in the ovary, quite similar to those found in NPA-induced fruits. All these results suggest that auxin can have opposing effects on fruit-set, either inducing (when accumulated in the ovary) or repressing (when transported from the apical shoot) that process, and that GAs act as mediators in both cases. The effect of NPA application and decapitation on fruit-set induction was also observed in MicroTom lines bearing introgressed DWARF and SELF-PRUNING wild-type alleles. SN - 1532-2548 UR - https://www.unboundmedicine.com/medline/citation/20388661/Inhibition_of_auxin_transport_from_the_ovary_or_from_the_apical_shoot_induces_parthenocarpic_fruit_set_in_tomato_mediated_by_gibberellins_ L2 - https://academic.oup.com/plphys/article-lookup/doi/10.1104/pp.110.155424 DB - PRIME DP - Unbound Medicine ER -