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A plasmid encoding parts of the dengue virus E and NS1 proteins induces an immune response in a mouse model.
Arch Virol. 2010 Jun; 155(6):847-56.AV

Abstract

A DENV-2 plasmid named pEII*EIII/NS1*,containing sequences encoding portions of the envelope protein that are potentially involved in the induction of neutralizing antibodies and a portion of the NS1 sequence that is involved in protection, is reported in this work. The synthesized subunit protein was recognized by human sera from infected patients and had the predicted size. The immunogenicity of this construct was evaluated using a mouse model in a prime-boost vaccination approach. The priming was performed using the plasmid pEII*EIII/NS1*, followed by a boost with recombinant full-length GST-E and GST-NS1 fusion proteins. The mice showed specific antibody responses to the E and NS1 proteins, as detected by ELISA, compared to the response of animals vaccinated with the parental plasmid. Interestingly, some animals had neutralizing antibodies. These results show that EII*, EIII and NS1* sequences could be considered for the design ofa recombinant subunit vaccine against dengue disease.

Authors+Show Affiliations

Departamento de Biomedicina Molecular, CINVESTAV IPN, Av. IPN # 2508 Col. San Pedro Zacatenco, México D. F., Mexico.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20390312

Citation

Mellado-Sánchez, Gabriela, et al. "A Plasmid Encoding Parts of the Dengue Virus E and NS1 Proteins Induces an Immune Response in a Mouse Model." Archives of Virology, vol. 155, no. 6, 2010, pp. 847-56.
Mellado-Sánchez G, García-Machorro J, Sandoval-Montes C, et al. A plasmid encoding parts of the dengue virus E and NS1 proteins induces an immune response in a mouse model. Arch Virol. 2010;155(6):847-56.
Mellado-Sánchez, G., García-Machorro, J., Sandoval-Montes, C., Gutiérrez-Castañeda, B., Rojo-Domínguez, A., García-Cordero, J., Santos-Argumedo, L., & Cedillo-Barrón, L. (2010). A plasmid encoding parts of the dengue virus E and NS1 proteins induces an immune response in a mouse model. Archives of Virology, 155(6), 847-56. https://doi.org/10.1007/s00705-010-0652-x
Mellado-Sánchez G, et al. A Plasmid Encoding Parts of the Dengue Virus E and NS1 Proteins Induces an Immune Response in a Mouse Model. Arch Virol. 2010;155(6):847-56. PubMed PMID: 20390312.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A plasmid encoding parts of the dengue virus E and NS1 proteins induces an immune response in a mouse model. AU - Mellado-Sánchez,Gabriela, AU - García-Machorro,Jazmín, AU - Sandoval-Montes,Claudia, AU - Gutiérrez-Castañeda,Benito, AU - Rojo-Domínguez,Arturo, AU - García-Cordero,Julio, AU - Santos-Argumedo,Leopoldo, AU - Cedillo-Barrón,Leticia, PY - 2009/08/04/received PY - 2010/02/24/accepted PY - 2010/4/15/entrez PY - 2010/4/15/pubmed PY - 2010/7/9/medline SP - 847 EP - 56 JF - Archives of virology JO - Arch Virol VL - 155 IS - 6 N2 - A DENV-2 plasmid named pEII*EIII/NS1*,containing sequences encoding portions of the envelope protein that are potentially involved in the induction of neutralizing antibodies and a portion of the NS1 sequence that is involved in protection, is reported in this work. The synthesized subunit protein was recognized by human sera from infected patients and had the predicted size. The immunogenicity of this construct was evaluated using a mouse model in a prime-boost vaccination approach. The priming was performed using the plasmid pEII*EIII/NS1*, followed by a boost with recombinant full-length GST-E and GST-NS1 fusion proteins. The mice showed specific antibody responses to the E and NS1 proteins, as detected by ELISA, compared to the response of animals vaccinated with the parental plasmid. Interestingly, some animals had neutralizing antibodies. These results show that EII*, EIII and NS1* sequences could be considered for the design ofa recombinant subunit vaccine against dengue disease. SN - 1432-8798 UR - https://www.unboundmedicine.com/medline/citation/20390312/A_plasmid_encoding_parts_of_the_dengue_virus_E_and_NS1_proteins_induces_an_immune_response_in_a_mouse_model_ L2 - https://dx.doi.org/10.1007/s00705-010-0652-x DB - PRIME DP - Unbound Medicine ER -