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Development of excitatory and inhibitory neurotransmitters in transitory cholinergic neurons, starburst amacrine cells, and GABAergic amacrine cells of rabbit retina, with implications for previsual and visual development of retinal ganglion cells.
Vis Neurosci 2010; 27(1-2):19-42VN

Abstract

Starburst amacrine cells (SACs), the only acetylcholine (ACh)-releasing amacrine cells (ACs) in adult rabbit retina, contain GABA and are key elements in the retina's directionally selective (DS) mechanism. Unlike many other GABAergic ACs, they use glutamic acid decarboxlyase (GAD)(67), not GAD(65), to synthesize GABA. Using immunocytochemistry, we demonstrate the apoptosis at birth (P0) of transitory putative ACs that exhibit immunoreactivity (IR) for the ACh-synthetic enzyme choline acetyltransferase (ChAT), GAD(67), and the GABA transporter, GAT1. Only a few intact, displaced ChAT-immunoreactive SAC bodies are detected at P0. At P2, ChAT-IR is detected in the two narrowly stratified substrata of starburst dendrites in the inner plexiform layer (IPL). Quantitative analysis reveals that in the first postnatal week, only a small fraction of SACs cells express ChAT- and GABA-IR. Not until the end of the second week are they expressed in all SACs. At P0, a three-tiered stratification of GABA-IR is present in the IPL, entirely different from the adult pattern of seven substrata, emerging at P3-P4, and optimally visualized at P13. At P0, GAD(65) is detectable in normally placed AC bodies. At P1, GAD(65)-IR appears in dendrites of nonstarburst GABAergic ACs, and by P5 is robust in the adult pattern of four substrata in the IPL. GAD(65)-IR never co-localizes with ChAT-IR. In a temporal comparison of our data with physiological, pharmacological, and ultrastructural studies, we suggest that transitory ChAT-immunoreactive cells share with SACs production of stage II (nicotinic) waves of previsual synchronous activity in ganglion cells (GCs). Further, we conclude that (1) GAD(65)-immunoreactive, non-SAC GABAergic ACs are the most likely candidates responsible for the suppression of stage III (muscarinic/AMPA-kainate) waves and (2) DS responses first appear in DS GCs, when about 50% of SACs express ChAT- and GABA-IR, and in 100% of DS GCs, when expression occurs in all SACs.

Authors+Show Affiliations

Department of Surgery (Ophthalmology), Brown University, Providence, Rhode Island 02882, USA. edward_famiglietti@brown.eduNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20392300

Citation

Famiglietti, Edward V., and Sarah J. Sundquist. "Development of Excitatory and Inhibitory Neurotransmitters in Transitory Cholinergic Neurons, Starburst Amacrine Cells, and GABAergic Amacrine Cells of Rabbit Retina, With Implications for Previsual and Visual Development of Retinal Ganglion Cells." Visual Neuroscience, vol. 27, no. 1-2, 2010, pp. 19-42.
Famiglietti EV, Sundquist SJ. Development of excitatory and inhibitory neurotransmitters in transitory cholinergic neurons, starburst amacrine cells, and GABAergic amacrine cells of rabbit retina, with implications for previsual and visual development of retinal ganglion cells. Vis Neurosci. 2010;27(1-2):19-42.
Famiglietti, E. V., & Sundquist, S. J. (2010). Development of excitatory and inhibitory neurotransmitters in transitory cholinergic neurons, starburst amacrine cells, and GABAergic amacrine cells of rabbit retina, with implications for previsual and visual development of retinal ganglion cells. Visual Neuroscience, 27(1-2), pp. 19-42. doi:10.1017/S0952523810000052.
Famiglietti EV, Sundquist SJ. Development of Excitatory and Inhibitory Neurotransmitters in Transitory Cholinergic Neurons, Starburst Amacrine Cells, and GABAergic Amacrine Cells of Rabbit Retina, With Implications for Previsual and Visual Development of Retinal Ganglion Cells. Vis Neurosci. 2010;27(1-2):19-42. PubMed PMID: 20392300.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of excitatory and inhibitory neurotransmitters in transitory cholinergic neurons, starburst amacrine cells, and GABAergic amacrine cells of rabbit retina, with implications for previsual and visual development of retinal ganglion cells. AU - Famiglietti,Edward V, AU - Sundquist,Sarah J, Y1 - 2010/04/15/ PY - 2010/4/16/entrez PY - 2010/4/16/pubmed PY - 2010/9/24/medline SP - 19 EP - 42 JF - Visual neuroscience JO - Vis. Neurosci. VL - 27 IS - 1-2 N2 - Starburst amacrine cells (SACs), the only acetylcholine (ACh)-releasing amacrine cells (ACs) in adult rabbit retina, contain GABA and are key elements in the retina's directionally selective (DS) mechanism. Unlike many other GABAergic ACs, they use glutamic acid decarboxlyase (GAD)(67), not GAD(65), to synthesize GABA. Using immunocytochemistry, we demonstrate the apoptosis at birth (P0) of transitory putative ACs that exhibit immunoreactivity (IR) for the ACh-synthetic enzyme choline acetyltransferase (ChAT), GAD(67), and the GABA transporter, GAT1. Only a few intact, displaced ChAT-immunoreactive SAC bodies are detected at P0. At P2, ChAT-IR is detected in the two narrowly stratified substrata of starburst dendrites in the inner plexiform layer (IPL). Quantitative analysis reveals that in the first postnatal week, only a small fraction of SACs cells express ChAT- and GABA-IR. Not until the end of the second week are they expressed in all SACs. At P0, a three-tiered stratification of GABA-IR is present in the IPL, entirely different from the adult pattern of seven substrata, emerging at P3-P4, and optimally visualized at P13. At P0, GAD(65) is detectable in normally placed AC bodies. At P1, GAD(65)-IR appears in dendrites of nonstarburst GABAergic ACs, and by P5 is robust in the adult pattern of four substrata in the IPL. GAD(65)-IR never co-localizes with ChAT-IR. In a temporal comparison of our data with physiological, pharmacological, and ultrastructural studies, we suggest that transitory ChAT-immunoreactive cells share with SACs production of stage II (nicotinic) waves of previsual synchronous activity in ganglion cells (GCs). Further, we conclude that (1) GAD(65)-immunoreactive, non-SAC GABAergic ACs are the most likely candidates responsible for the suppression of stage III (muscarinic/AMPA-kainate) waves and (2) DS responses first appear in DS GCs, when about 50% of SACs express ChAT- and GABA-IR, and in 100% of DS GCs, when expression occurs in all SACs. SN - 1469-8714 UR - https://www.unboundmedicine.com/medline/citation/20392300/Development_of_excitatory_and_inhibitory_neurotransmitters_in_transitory_cholinergic_neurons_starburst_amacrine_cells_and_GABAergic_amacrine_cells_of_rabbit_retina_with_implications_for_previsual_and_visual_development_of_retinal_ganglion_cells_ L2 - https://www.cambridge.org/core/product/identifier/S0952523810000052/type/journal_article DB - PRIME DP - Unbound Medicine ER -