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Ribosomal protein L35 is required for 27SB pre-rRNA processing in Saccharomyces cerevisiae.
Nucleic Acids Res. 2010 Aug; 38(15):5177-92.NA

Abstract

Ribosome synthesis involves the concomitance of pre-rRNA processing and ribosomal protein assembly. In eukaryotes, this is a complex process that requires the participation of specific sequences and structures within the pre-rRNAs, at least 200 trans-acting factors and the ribosomal proteins. There is little information on the function of individual 60S ribosomal proteins in ribosome synthesis. Herein, we have analysed the contribution of ribosomal protein L35 in ribosome biogenesis. In vivo depletion of L35 results in a deficit in 60S ribosomal subunits and the appearance of half-mer polysomes. Pulse-chase, northern hybridization and primer extension analyses show that processing of the 27SB to 7S pre-rRNAs is strongly delayed upon L35 depletion. Most likely as a consequence of this, release of pre-60S ribosomal particles from the nucleolus to the nucleoplasm is also blocked. Deletion of RPL35A leads to similar although less pronounced phenotypes. Moreover, we show that L35 assembles in the nucleolus and binds to early pre-60S ribosomal particles. Finally, flow cytometry analysis indicated that L35-depleted cells mildly delay the G1 phase of the cell cycle. We conclude that L35 assembly is a prerequisite for the efficient cleavage of the internal transcribed spacer 2 at site C(2).

Authors+Show Affiliations

Departamento de Genética, Universidad de Sevilla, Sevilla, Spain.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20392820

Citation

Babiano, Reyes, and Jesús de la Cruz. "Ribosomal Protein L35 Is Required for 27SB pre-rRNA Processing in Saccharomyces Cerevisiae." Nucleic Acids Research, vol. 38, no. 15, 2010, pp. 5177-92.
Babiano R, de la Cruz J. Ribosomal protein L35 is required for 27SB pre-rRNA processing in Saccharomyces cerevisiae. Nucleic Acids Res. 2010;38(15):5177-92.
Babiano, R., & de la Cruz, J. (2010). Ribosomal protein L35 is required for 27SB pre-rRNA processing in Saccharomyces cerevisiae. Nucleic Acids Research, 38(15), 5177-92. https://doi.org/10.1093/nar/gkq260
Babiano R, de la Cruz J. Ribosomal Protein L35 Is Required for 27SB pre-rRNA Processing in Saccharomyces Cerevisiae. Nucleic Acids Res. 2010;38(15):5177-92. PubMed PMID: 20392820.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ribosomal protein L35 is required for 27SB pre-rRNA processing in Saccharomyces cerevisiae. AU - Babiano,Reyes, AU - de la Cruz,Jesús, Y1 - 2010/04/14/ PY - 2010/4/16/entrez PY - 2010/4/16/pubmed PY - 2010/9/30/medline SP - 5177 EP - 92 JF - Nucleic acids research JO - Nucleic Acids Res. VL - 38 IS - 15 N2 - Ribosome synthesis involves the concomitance of pre-rRNA processing and ribosomal protein assembly. In eukaryotes, this is a complex process that requires the participation of specific sequences and structures within the pre-rRNAs, at least 200 trans-acting factors and the ribosomal proteins. There is little information on the function of individual 60S ribosomal proteins in ribosome synthesis. Herein, we have analysed the contribution of ribosomal protein L35 in ribosome biogenesis. In vivo depletion of L35 results in a deficit in 60S ribosomal subunits and the appearance of half-mer polysomes. Pulse-chase, northern hybridization and primer extension analyses show that processing of the 27SB to 7S pre-rRNAs is strongly delayed upon L35 depletion. Most likely as a consequence of this, release of pre-60S ribosomal particles from the nucleolus to the nucleoplasm is also blocked. Deletion of RPL35A leads to similar although less pronounced phenotypes. Moreover, we show that L35 assembles in the nucleolus and binds to early pre-60S ribosomal particles. Finally, flow cytometry analysis indicated that L35-depleted cells mildly delay the G1 phase of the cell cycle. We conclude that L35 assembly is a prerequisite for the efficient cleavage of the internal transcribed spacer 2 at site C(2). SN - 1362-4962 UR - https://www.unboundmedicine.com/medline/citation/20392820/Ribosomal_protein_L35_is_required_for_27SB_pre_rRNA_processing_in_Saccharomyces_cerevisiae_ L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkq260 DB - PRIME DP - Unbound Medicine ER -