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[Collagen spatial distribution in rapid atrial pacing dogs with or without superior vena cava and aortic root fat pad].
Zhonghua Xin Xue Guan Bing Za Zhi. 2010 Feb; 38(2):171-4.ZX

Abstract

OBJECTIVE

To observe the collagen spatial distribution, collagen volume fraction (CVF) and Cx40, Cx43mRNA expressions in rapid atrial pacing dogs post vagal denervation by removing fat pad located between the medial superior vena cava and aortic root (SVC-Ao fat pad).

METHODS

Twenty-four dogs were randomly divided into unpaced sham operation group (S group, n = 8), Keeping SVC-Ao fat pad group (K group, n = 8) and Removing SVC-Ao fat pad group (R group, SVC-Ao fat pad was removed by surgical excision before pacing, n = 8). K and R groups were paced for six weeks. Six weeks later, all dogs were sacrificed, left atrium (LA), right atrium (RA), left atrial appendage (LAA), right atrial appendage (RAA) and atrial septum (AS) were collected and stained with HE or Masson Trichrome or frozen in liquid nitrogen for quantifying the expression of Cx40, Cx43 mRNA by Real-time quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR).

RESULTS

Spatial distribution of collagen fibers as well as CVF between S and R group were similar (all P > 0.05). CVF was significantly higher in K group compared to R group, especially at LAA and AS locations (all P < 0.05). Cx40mRNA expression in K group was significantly decreased in LA, RA, and significantly increased in LAA, RAA and AS compared those in S group (all P < 0.05), significantly lower in LA and RA while significantly higher in LAA and RAA compared to R group (all P < 0.05). Cx43mRNA expression in K group was significantly reduced in LA, RA, LAA and RAA while significantly increased in AS compared to S group (all P < 0.05), significantly higher in LA, RA, RAA and AS while significantly lower in LAA compared to R group.

CONCLUSION

Pacing induced collagen remodeling and modulation on Cx40mRNA and Cx43 mRNA expressions could be partially attenuated by removing SVC-Ao fat pad suggesting vagal denervation plays a key role in the initiation and preservation of atrial fibrillation.

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Publisher Full Text

Authors+Show Affiliations

Xiong RX
Department of Cardiology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
Zhong GQ
No affiliation info available
Song HX
No affiliation info available
Zhang JC
No affiliation info available
Ling Y
No affiliation info available

MeSH

AnimalsAtrial FibrillationCardiac Pacing, ArtificialCollagenConnexin 43ConnexinsDogsHeart AtriaRNA, Messenger

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

20398567

Citation

Xiong, Ri-Xin, et al. "[Collagen Spatial Distribution in Rapid Atrial Pacing Dogs With or Without Superior Vena Cava and Aortic Root Fat Pad]." Zhonghua Xin Xue Guan Bing Za Zhi, vol. 38, no. 2, 2010, pp. 171-4.
Xiong RX, Zhong GQ, Song HX, et al. [Collagen spatial distribution in rapid atrial pacing dogs with or without superior vena cava and aortic root fat pad]. Zhonghua Xin Xue Guan Bing Za Zhi. 2010;38(2):171-4.
Xiong, R. X., Zhong, G. Q., Song, H. X., Zhang, J. C., & Ling, Y. (2010). [Collagen spatial distribution in rapid atrial pacing dogs with or without superior vena cava and aortic root fat pad]. Zhonghua Xin Xue Guan Bing Za Zhi, 38(2), 171-4.
Xiong RX, et al. [Collagen Spatial Distribution in Rapid Atrial Pacing Dogs With or Without Superior Vena Cava and Aortic Root Fat Pad]. Zhonghua Xin Xue Guan Bing Za Zhi. 2010;38(2):171-4. PubMed PMID: 20398567.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Collagen spatial distribution in rapid atrial pacing dogs with or without superior vena cava and aortic root fat pad]. AU - Xiong,Ri-Xin, AU - Zhong,Guo-Qiang, AU - Song,Hong-Xing, AU - Zhang,Jing-Chang, AU - Ling,Yun, PY - 2010/4/20/entrez PY - 2010/4/20/pubmed PY - 2011/10/14/medline SP - 171 EP - 4 JF - Zhonghua xin xue guan bing za zhi JO - Zhonghua Xin Xue Guan Bing Za Zhi VL - 38 IS - 2 N2 - OBJECTIVE: To observe the collagen spatial distribution, collagen volume fraction (CVF) and Cx40, Cx43mRNA expressions in rapid atrial pacing dogs post vagal denervation by removing fat pad located between the medial superior vena cava and aortic root (SVC-Ao fat pad). METHODS: Twenty-four dogs were randomly divided into unpaced sham operation group (S group, n = 8), Keeping SVC-Ao fat pad group (K group, n = 8) and Removing SVC-Ao fat pad group (R group, SVC-Ao fat pad was removed by surgical excision before pacing, n = 8). K and R groups were paced for six weeks. Six weeks later, all dogs were sacrificed, left atrium (LA), right atrium (RA), left atrial appendage (LAA), right atrial appendage (RAA) and atrial septum (AS) were collected and stained with HE or Masson Trichrome or frozen in liquid nitrogen for quantifying the expression of Cx40, Cx43 mRNA by Real-time quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR). RESULTS: Spatial distribution of collagen fibers as well as CVF between S and R group were similar (all P > 0.05). CVF was significantly higher in K group compared to R group, especially at LAA and AS locations (all P < 0.05). Cx40mRNA expression in K group was significantly decreased in LA, RA, and significantly increased in LAA, RAA and AS compared those in S group (all P < 0.05), significantly lower in LA and RA while significantly higher in LAA and RAA compared to R group (all P < 0.05). Cx43mRNA expression in K group was significantly reduced in LA, RA, LAA and RAA while significantly increased in AS compared to S group (all P < 0.05), significantly higher in LA, RA, RAA and AS while significantly lower in LAA compared to R group. CONCLUSION: Pacing induced collagen remodeling and modulation on Cx40mRNA and Cx43 mRNA expressions could be partially attenuated by removing SVC-Ao fat pad suggesting vagal denervation plays a key role in the initiation and preservation of atrial fibrillation. SN - 0253-3758 UR - https://www.unboundmedicine.com/medline/citation/20398567/[Collagen_spatial_distribution_in_rapid_atrial_pacing_dogs_with_or_without_superior_vena_cava_and_aortic_root_fat_pad]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0253-3758&amp;year=2010&amp;vol=38&amp;issue=2&amp;fpage=171 DB - PRIME DP - Unbound Medicine ER -