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A highly sensitive detection platform based on surface-enhanced Raman scattering for Escherichia coli enumeration.
Anal Bioanal Chem. 2010 Jun; 397(4):1595-604.AB

Abstract

A very sensitive and highly specific heterogeneous immunoassay system, based on surface-enhanced Raman scattering (SERS) and gold nanoparticles, was developed for the detection of bacteria and other pathogens. Two different types of gold nanoparticles (citrate-stabilized gold nanosphere and hexadecyltrimethylammonium bromide (CTAB)-stabilized gold nanorod particles) were examined and this immunoassay was applied for the detection of Escherichia coli. Raman labels were constructed by using these spherical and rod-shaped gold nanoparticles which were first coated with 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and subsequently with a molecular recognizer. The working curve was obtained by plotting the intensity of the SERS signal of the symmetric NO(2) stretching of DTNB at 1,333 cm(-1) versus the concentration of the E. coli. The analytical performance of gold particles was evaluated via a sandwich immunoassay, and linear calibration graphs were obtained in the E. coli concentration range of 10(1)-10(5) cfu/mL with a 60-s accumulation time. The sensitivity of the Raman label fabricated with gold nanorods was more than three times higher than spherical gold nanoparticles. The selectivity of the developed sensor was examined with Enterobacter aerogenes and Enterobacter dissolvens, which did not produce any significant response. The usefulness of the developed immunoassay to detect E. coli in real water samples was also demonstrated.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20401720

Citation

Temur, Erhan, et al. "A Highly Sensitive Detection Platform Based On Surface-enhanced Raman Scattering for Escherichia Coli Enumeration." Analytical and Bioanalytical Chemistry, vol. 397, no. 4, 2010, pp. 1595-604.
Temur E, Boyaci IH, Tamer U, et al. A highly sensitive detection platform based on surface-enhanced Raman scattering for Escherichia coli enumeration. Anal Bioanal Chem. 2010;397(4):1595-604.
Temur, E., Boyaci, I. H., Tamer, U., Unsal, H., & Aydogan, N. (2010). A highly sensitive detection platform based on surface-enhanced Raman scattering for Escherichia coli enumeration. Analytical and Bioanalytical Chemistry, 397(4), 1595-604. https://doi.org/10.1007/s00216-010-3676-x
Temur E, et al. A Highly Sensitive Detection Platform Based On Surface-enhanced Raman Scattering for Escherichia Coli Enumeration. Anal Bioanal Chem. 2010;397(4):1595-604. PubMed PMID: 20401720.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A highly sensitive detection platform based on surface-enhanced Raman scattering for Escherichia coli enumeration. AU - Temur,Erhan, AU - Boyaci,Ismail Hakki, AU - Tamer,Uğur, AU - Unsal,Hande, AU - Aydogan,Nihal, Y1 - 2010/04/18/ PY - 2010/01/07/received PY - 2010/03/22/accepted PY - 2010/03/22/revised PY - 2010/4/20/entrez PY - 2010/4/20/pubmed PY - 2010/9/15/medline SP - 1595 EP - 604 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 397 IS - 4 N2 - A very sensitive and highly specific heterogeneous immunoassay system, based on surface-enhanced Raman scattering (SERS) and gold nanoparticles, was developed for the detection of bacteria and other pathogens. Two different types of gold nanoparticles (citrate-stabilized gold nanosphere and hexadecyltrimethylammonium bromide (CTAB)-stabilized gold nanorod particles) were examined and this immunoassay was applied for the detection of Escherichia coli. Raman labels were constructed by using these spherical and rod-shaped gold nanoparticles which were first coated with 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and subsequently with a molecular recognizer. The working curve was obtained by plotting the intensity of the SERS signal of the symmetric NO(2) stretching of DTNB at 1,333 cm(-1) versus the concentration of the E. coli. The analytical performance of gold particles was evaluated via a sandwich immunoassay, and linear calibration graphs were obtained in the E. coli concentration range of 10(1)-10(5) cfu/mL with a 60-s accumulation time. The sensitivity of the Raman label fabricated with gold nanorods was more than three times higher than spherical gold nanoparticles. The selectivity of the developed sensor was examined with Enterobacter aerogenes and Enterobacter dissolvens, which did not produce any significant response. The usefulness of the developed immunoassay to detect E. coli in real water samples was also demonstrated. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/20401720/A_highly_sensitive_detection_platform_based_on_surface_enhanced_Raman_scattering_for_Escherichia_coli_enumeration_ L2 - https://dx.doi.org/10.1007/s00216-010-3676-x DB - PRIME DP - Unbound Medicine ER -