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Active infection of human blood monocytes by Chikungunya virus triggers an innate immune response.
J Immunol. 2010 May 15; 184(10):5903-13.JI

Abstract

Chikungunya virus (CHIKV) is an alphavirus that causes chronic and incapacitating arthralgia in humans. To date, interactions between the immune system and the different stages of the virus life cycle remain poorly defined. We demonstrated for the first time that CHIKV Ags could be detected in vivo in the monocytes of acutely infected patients. Using in vitro experimental systems, whole blood and purified monocytes, we confirmed that monocytes could be infected and virus growth could be sustained. CHIKV interactions with monocytes, and with other blood leukocytes, induced a robust and rapid innate immune response with the production of specific chemokines and cytokines. In particular, high levels of IFN-alpha were produced rapidly after CHIKV incubation with monocytes. The identification of monocytes during the early phase of CHIKV infection in vivo is significant as infected monocyte/macrophage cells have been detected in the synovial tissues of chronically CHIKV-infected patients, and these cells may behave as the vehicles for virus dissemination. This may explain the persistence of joint symptoms despite the short duration of viremia. Our results provide a better understanding on the basic mechanisms of infection and early antiviral immune responses and will help in the development of future effective control strategies.

Authors+Show Affiliations

Singapore Immunology Network, Agency for Science, Technology and Research, Biopolis, Singapore.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20404274

Citation

Her, Zhisheng, et al. "Active Infection of Human Blood Monocytes By Chikungunya Virus Triggers an Innate Immune Response." Journal of Immunology (Baltimore, Md. : 1950), vol. 184, no. 10, 2010, pp. 5903-13.
Her Z, Malleret B, Chan M, et al. Active infection of human blood monocytes by Chikungunya virus triggers an innate immune response. J Immunol. 2010;184(10):5903-13.
Her, Z., Malleret, B., Chan, M., Ong, E. K., Wong, S. C., Kwek, D. J., Tolou, H., Lin, R. T., Tambyah, P. A., Rénia, L., & Ng, L. F. (2010). Active infection of human blood monocytes by Chikungunya virus triggers an innate immune response. Journal of Immunology (Baltimore, Md. : 1950), 184(10), 5903-13. https://doi.org/10.4049/jimmunol.0904181
Her Z, et al. Active Infection of Human Blood Monocytes By Chikungunya Virus Triggers an Innate Immune Response. J Immunol. 2010 May 15;184(10):5903-13. PubMed PMID: 20404274.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Active infection of human blood monocytes by Chikungunya virus triggers an innate immune response. AU - Her,Zhisheng, AU - Malleret,Benoit, AU - Chan,Monica, AU - Ong,Edward K S, AU - Wong,Siew-Cheng, AU - Kwek,Dyan J C, AU - Tolou,Hugues, AU - Lin,Raymond T P, AU - Tambyah,Paul Anantharajah, AU - Rénia,Laurent, AU - Ng,Lisa F P, Y1 - 2010/04/19/ PY - 2010/4/21/entrez PY - 2010/4/21/pubmed PY - 2010/7/20/medline SP - 5903 EP - 13 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 184 IS - 10 N2 - Chikungunya virus (CHIKV) is an alphavirus that causes chronic and incapacitating arthralgia in humans. To date, interactions between the immune system and the different stages of the virus life cycle remain poorly defined. We demonstrated for the first time that CHIKV Ags could be detected in vivo in the monocytes of acutely infected patients. Using in vitro experimental systems, whole blood and purified monocytes, we confirmed that monocytes could be infected and virus growth could be sustained. CHIKV interactions with monocytes, and with other blood leukocytes, induced a robust and rapid innate immune response with the production of specific chemokines and cytokines. In particular, high levels of IFN-alpha were produced rapidly after CHIKV incubation with monocytes. The identification of monocytes during the early phase of CHIKV infection in vivo is significant as infected monocyte/macrophage cells have been detected in the synovial tissues of chronically CHIKV-infected patients, and these cells may behave as the vehicles for virus dissemination. This may explain the persistence of joint symptoms despite the short duration of viremia. Our results provide a better understanding on the basic mechanisms of infection and early antiviral immune responses and will help in the development of future effective control strategies. SN - 1550-6606 UR - https://www.unboundmedicine.com/medline/citation/20404274/Active_infection_of_human_blood_monocytes_by_Chikungunya_virus_triggers_an_innate_immune_response_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=20404274 DB - PRIME DP - Unbound Medicine ER -