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Dengue NS1 antigen detection: a useful tool in early diagnosis of dengue virus infection.
Indian J Med Microbiol. 2010 Apr-Jun; 28(2):107-10.IJ

Abstract

PURPOSE

This study was undertaken to evaluate the efficacy of NS1 antigen (Ag) assay as an early marker for dengue virus (DV) infection.

MATERIALS AND METHODS

Group I evaluated the performance of NS1 antigen (Ag) assay in comparison to MAC-ELISA and their detection rate when performed together in a single sample. Six hundred acute/early convalescent sera were screened by both the assays. Group II evaluated the efficacy of a single assay in 30 acute phase sera of paediatric OPD patients screened only by NS1 Ag assay. Group III evaluated the specificity of NS1 assay in comparison to MAC-ELISA on 40 samples included as controls.

RESULTS

In Group I, 140 (23.3%) and 235 (39.1%) samples were positive by NS1 assay and MAC-ELISA respectively. The detection rate increased to 320 (53.3%) when both the assays were used together on a single sample. NS1 Ag positivity varied from 71.42% to 28.4% in acute and early convalescent sera, conversely IgM detection rate was 93.61% and 6.38% in early convalescent and acute phase sera respectively (P < 0.0001). In Group II, 66.66% (20) samples were positive by NS1 assay. All the samples in Group III were negative showing 100% specificity of both the assays.

CONCLUSION

NS1 Ag assay holds promise in early diagnosis of dengue infection. When used in combination with MAC-ELISA on a single sample it significantly improves the diagnostic algorithm without the requirement of paired sera.

Authors+Show Affiliations

Department of Clinical Microbiology, Sir Ganga Ram Hospital, Rajinder Nagar, New Delhi - 110 060, India.No affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

20404453

Citation

Datta, S, and C Wattal. "Dengue NS1 Antigen Detection: a Useful Tool in Early Diagnosis of Dengue Virus Infection." Indian Journal of Medical Microbiology, vol. 28, no. 2, 2010, pp. 107-10.
Datta S, Wattal C. Dengue NS1 antigen detection: a useful tool in early diagnosis of dengue virus infection. Indian J Med Microbiol. 2010;28(2):107-10.
Datta, S., & Wattal, C. (2010). Dengue NS1 antigen detection: a useful tool in early diagnosis of dengue virus infection. Indian Journal of Medical Microbiology, 28(2), 107-10. https://doi.org/10.4103/0255-0857.62484
Datta S, Wattal C. Dengue NS1 Antigen Detection: a Useful Tool in Early Diagnosis of Dengue Virus Infection. Indian J Med Microbiol. 2010 Apr-Jun;28(2):107-10. PubMed PMID: 20404453.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dengue NS1 antigen detection: a useful tool in early diagnosis of dengue virus infection. AU - Datta,S, AU - Wattal,C, PY - 2010/4/21/entrez PY - 2010/4/21/pubmed PY - 2010/7/14/medline SP - 107 EP - 10 JF - Indian journal of medical microbiology JO - Indian J Med Microbiol VL - 28 IS - 2 N2 - PURPOSE: This study was undertaken to evaluate the efficacy of NS1 antigen (Ag) assay as an early marker for dengue virus (DV) infection. MATERIALS AND METHODS: Group I evaluated the performance of NS1 antigen (Ag) assay in comparison to MAC-ELISA and their detection rate when performed together in a single sample. Six hundred acute/early convalescent sera were screened by both the assays. Group II evaluated the efficacy of a single assay in 30 acute phase sera of paediatric OPD patients screened only by NS1 Ag assay. Group III evaluated the specificity of NS1 assay in comparison to MAC-ELISA on 40 samples included as controls. RESULTS: In Group I, 140 (23.3%) and 235 (39.1%) samples were positive by NS1 assay and MAC-ELISA respectively. The detection rate increased to 320 (53.3%) when both the assays were used together on a single sample. NS1 Ag positivity varied from 71.42% to 28.4% in acute and early convalescent sera, conversely IgM detection rate was 93.61% and 6.38% in early convalescent and acute phase sera respectively (P < 0.0001). In Group II, 66.66% (20) samples were positive by NS1 assay. All the samples in Group III were negative showing 100% specificity of both the assays. CONCLUSION: NS1 Ag assay holds promise in early diagnosis of dengue infection. When used in combination with MAC-ELISA on a single sample it significantly improves the diagnostic algorithm without the requirement of paired sera. SN - 1998-3646 UR - https://www.unboundmedicine.com/medline/citation/20404453/Dengue_NS1_antigen_detection:_a_useful_tool_in_early_diagnosis_of_dengue_virus_infection_ L2 - http://www.ijmm.org/article.asp?issn=0255-0857;year=2010;volume=28;issue=2;spage=107;epage=110;aulast=Datta DB - PRIME DP - Unbound Medicine ER -