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Apoptosis caused by an inhibitor of NO production in the decidua of rat from mid-gestation.
Exp Biol Med (Maywood). 2010 Apr; 235(4):455-62.EB

Abstract

We previously reported that nitric oxide (NO) is first detected in the uterus of a pregnant rat on gestational day 13.5 (GD13.5) and that NO levels peak on GD17.5. In addition, NO production in the uterus is mainly derived from the decidua and not the myometrium. The aim of the present study was to reveal the role of NO that peaked on GD17.5 of gestation in the decidua. To inhibit NO production, pregnant rats were continuously administered by an nitric oxide synthase inhibitor, N(G)-nitro-L-arginine-methyl ester (L-NAME) for 48 h. In the control group, saline was infused instead of L-NAME. After treatment, the decidua were obtained from GD13.5, GD17.5 and GD21.5 rats. Apoptosis and activated caspase-3-positive cells were observed by transferase-mediated dUTP nick-end labeling (TUNEL) assay and immunohistochemistry, respectively. The caspase-3 enzyme activity was also measured in the cell lysate from the decidua. The numbers of TUNEL-positive cells and activated caspase-3-positive cells each increased and the amount of caspase-3 activity also increased significantly in rats on GD17.5 than in rats in the control group, but no changes were observed in rats on GD13.5 and GD21.5. Furthermore, enzyme activity regarding the initiator caspases, caspase-8 and -9, upstream factors for caspase-3 in the caspase cascade, was measured simultaneously on GD17.5 under the same treatment. Caspase-8 and -9 enzyme activities increased significantly in the control group; an increment of caspase-8 activity was especially prominent. The present results indicate that an inhibitor of NO production caused apoptosis through typical apoptotic signals in the decidua on GD17.5, suggesting that an NO peak in the decidua is essential to cell survival and the maintenance of uterine formation.

Authors+Show Affiliations

Graduate School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 229-8501, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20407077

Citation

Suzuki, Takehito, et al. "Apoptosis Caused By an Inhibitor of NO Production in the Decidua of Rat From Mid-gestation." Experimental Biology and Medicine (Maywood, N.J.), vol. 235, no. 4, 2010, pp. 455-62.
Suzuki T, Nagamatsu C, Kushima T, et al. Apoptosis caused by an inhibitor of NO production in the decidua of rat from mid-gestation. Exp Biol Med (Maywood). 2010;235(4):455-62.
Suzuki, T., Nagamatsu, C., Kushima, T., Miyakoshi, R., Tanaka, K., Morita, H., Sakaue, M., & Takizawa, T. (2010). Apoptosis caused by an inhibitor of NO production in the decidua of rat from mid-gestation. Experimental Biology and Medicine (Maywood, N.J.), 235(4), 455-62. https://doi.org/10.1258/ebm.2009.009285
Suzuki T, et al. Apoptosis Caused By an Inhibitor of NO Production in the Decidua of Rat From Mid-gestation. Exp Biol Med (Maywood). 2010;235(4):455-62. PubMed PMID: 20407077.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apoptosis caused by an inhibitor of NO production in the decidua of rat from mid-gestation. AU - Suzuki,Takehito, AU - Nagamatsu,Chiaki, AU - Kushima,Takahiro, AU - Miyakoshi,Ryu, AU - Tanaka,Kazuaki, AU - Morita,Hidetoshi, AU - Sakaue,Motoharu, AU - Takizawa,Tatsuya, PY - 2010/4/22/entrez PY - 2010/4/22/pubmed PY - 2010/4/29/medline SP - 455 EP - 62 JF - Experimental biology and medicine (Maywood, N.J.) JO - Exp. Biol. Med. (Maywood) VL - 235 IS - 4 N2 - We previously reported that nitric oxide (NO) is first detected in the uterus of a pregnant rat on gestational day 13.5 (GD13.5) and that NO levels peak on GD17.5. In addition, NO production in the uterus is mainly derived from the decidua and not the myometrium. The aim of the present study was to reveal the role of NO that peaked on GD17.5 of gestation in the decidua. To inhibit NO production, pregnant rats were continuously administered by an nitric oxide synthase inhibitor, N(G)-nitro-L-arginine-methyl ester (L-NAME) for 48 h. In the control group, saline was infused instead of L-NAME. After treatment, the decidua were obtained from GD13.5, GD17.5 and GD21.5 rats. Apoptosis and activated caspase-3-positive cells were observed by transferase-mediated dUTP nick-end labeling (TUNEL) assay and immunohistochemistry, respectively. The caspase-3 enzyme activity was also measured in the cell lysate from the decidua. The numbers of TUNEL-positive cells and activated caspase-3-positive cells each increased and the amount of caspase-3 activity also increased significantly in rats on GD17.5 than in rats in the control group, but no changes were observed in rats on GD13.5 and GD21.5. Furthermore, enzyme activity regarding the initiator caspases, caspase-8 and -9, upstream factors for caspase-3 in the caspase cascade, was measured simultaneously on GD17.5 under the same treatment. Caspase-8 and -9 enzyme activities increased significantly in the control group; an increment of caspase-8 activity was especially prominent. The present results indicate that an inhibitor of NO production caused apoptosis through typical apoptotic signals in the decidua on GD17.5, suggesting that an NO peak in the decidua is essential to cell survival and the maintenance of uterine formation. SN - 1535-3699 UR - https://www.unboundmedicine.com/medline/citation/20407077/Apoptosis_caused_by_an_inhibitor_of_NO_production_in_the_decidua_of_rat_from_mid_gestation_ L2 - http://journals.sagepub.com/doi/full/10.1258/ebm.2009.009285?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -