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Characterization of a wheat-Thinopyrum bessarabicum (T2JS-2BS.2BL) translocation line.
Theor Appl Genet. 2010 Aug; 121(3):589-97.TA

Abstract

Thinopyrum bessarabicum (2n = 2x = 14, JJ or E(b)E(b)) is an important genetic resource for wheat improvement due to its salinity tolerance and disease resistance. Development of wheat-Th. bessarabicum translocation lines will facilitate its practical utilization in wheat improvement. In this study, a novel wheat-Th. bessarabicum translocation line T2JS-2BS.2BL, which carries a segment of Th. bessarabicum chromosome arm 2JS was identified and further characterized using sequential chromosome C-banding, genomic in situ hybridization (GISH), dual-color fluorescent in situ hybridization (FISH) and DNA markers. The translocation breakpoint was mapped within bin C-2BS1-0.53 of chromosome 2B through marker analysis. Compared to the Chinese Spring (CS) parent and to CS-type lines, the translocation line has more fertile spikes per plant, longer spikes, more grains per spike and higher yield per plant, which suggests that the alien segment carries yield-related genes. However, plants with the translocation are also taller, head later and have lower 1,000-kernel weight than CS or CS-type lines. By using markers specific to the barley photoperiod response gene Ppd-H1, it was determined that the late heading date was conferred by a recessive allele located on the 2JS segment. In addition, four markers specific for the translocated segment were identified, which can be used for marker-aided screening.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Qi Z
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China. zjqi@njau.edu.cn
Du P
No affiliation info available
Qian B
No affiliation info available
Zhuang L
No affiliation info available
Chen H
No affiliation info available
Chen T
No affiliation info available
Shen J
No affiliation info available
Guo J
No affiliation info available
Feng Y
No affiliation info available
Pei Z
No affiliation info available

MeSH

Chromosome BandingChromosomes, PlantCrosses, GeneticEdible GrainGenetic MarkersHybrid VigorIn Situ Hybridization, FluorescencePoaceaeTranslocation, GeneticTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20407740

Citation

Qi, Zengjun, et al. "Characterization of a wheat-Thinopyrum Bessarabicum (T2JS-2BS.2BL) Translocation Line." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 121, no. 3, 2010, pp. 589-97.
Qi Z, Du P, Qian B, et al. Characterization of a wheat-Thinopyrum bessarabicum (T2JS-2BS.2BL) translocation line. Theor Appl Genet. 2010;121(3):589-97.
Qi, Z., Du, P., Qian, B., Zhuang, L., Chen, H., Chen, T., Shen, J., Guo, J., Feng, Y., & Pei, Z. (2010). Characterization of a wheat-Thinopyrum bessarabicum (T2JS-2BS.2BL) translocation line. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 121(3), 589-97. https://doi.org/10.1007/s00122-010-1332-7
Qi Z, et al. Characterization of a wheat-Thinopyrum Bessarabicum (T2JS-2BS.2BL) Translocation Line. Theor Appl Genet. 2010;121(3):589-97. PubMed PMID: 20407740.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of a wheat-Thinopyrum bessarabicum (T2JS-2BS.2BL) translocation line. AU - Qi,Zengjun, AU - Du,Pei, AU - Qian,Baoli, AU - Zhuang,Lifang, AU - Chen,Huafeng, AU - Chen,Tingting, AU - Shen,Jian, AU - Guo,Jie, AU - Feng,Yigao, AU - Pei,Ziyou, Y1 - 2010/04/21/ PY - 2009/11/16/received PY - 2010/04/01/accepted PY - 2010/4/22/entrez PY - 2010/4/22/pubmed PY - 2010/10/13/medline SP - 589 EP - 97 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 121 IS - 3 N2 - Thinopyrum bessarabicum (2n = 2x = 14, JJ or E(b)E(b)) is an important genetic resource for wheat improvement due to its salinity tolerance and disease resistance. Development of wheat-Th. bessarabicum translocation lines will facilitate its practical utilization in wheat improvement. In this study, a novel wheat-Th. bessarabicum translocation line T2JS-2BS.2BL, which carries a segment of Th. bessarabicum chromosome arm 2JS was identified and further characterized using sequential chromosome C-banding, genomic in situ hybridization (GISH), dual-color fluorescent in situ hybridization (FISH) and DNA markers. The translocation breakpoint was mapped within bin C-2BS1-0.53 of chromosome 2B through marker analysis. Compared to the Chinese Spring (CS) parent and to CS-type lines, the translocation line has more fertile spikes per plant, longer spikes, more grains per spike and higher yield per plant, which suggests that the alien segment carries yield-related genes. However, plants with the translocation are also taller, head later and have lower 1,000-kernel weight than CS or CS-type lines. By using markers specific to the barley photoperiod response gene Ppd-H1, it was determined that the late heading date was conferred by a recessive allele located on the 2JS segment. In addition, four markers specific for the translocated segment were identified, which can be used for marker-aided screening. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/20407740/Characterization_of_a_wheat_Thinopyrum_bessarabicum__T2JS_2BS_2BL__translocation_line_ L2 - https://dx.doi.org/10.1007/s00122-010-1332-7 DB - PRIME DP - Unbound Medicine ER -