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Utility of a commercial nonstructural protein 1 antigen capture kit as a dengue virus diagnostic tool.
Clin Vaccine Immunol. 2010 Jun; 17(6):949-53.CV

Abstract

Annually, over 2.5 billion people are at risk for infection with dengue virus (DENV), while between 50 and 100 million people contract the infection. There is an urgent need for alternative diagnostic tools that can detect DENV during acute infection. Recent studies have shown that DENV nonstructural protein 1 (NS1) is detectable in the blood as early as the onset of symptoms and persists well into the convalescent phase of the infection. We evaluated the utility of the Bio-Rad Platelia DENV NS1 antigen capture kit in combination with real-time reverse transcriptase PCR (RT-PCR) and an IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) for refining a new algorithm for the diagnosis of acute- or convalescent-phase DENV infection with a single clinical sample. We tested the Bio-Rad kit with three panels of sera. These panels were designed to evaluate the sensitivities of the NS1 kit for (i) early-convalescent-phase samples, (ii) acute-phase samples with false-negative PCR results, and (iii) IgM-negative convalescent-phase samples from patients with confirmed secondary DENV infections. Results show that NS1 can be detected in 22% of serum samples collected more than 10 days after the onset of illness and in 22% of samples that did not elicit an IgM response. Additionally, NS1 was detected in 37% of the tested acute-phase samples with false-negative PCR results, suggesting that NS1 detection may be valuable in increasing the sensitivity of current acute-phase diagnostics. These results will improve diagnosis with a single acute-phase or early-convalescent-phase sample for disease surveillance and clinical diagnosis.

Authors+Show Affiliations

Public Health Service, CDC-Dengue Branch, 1324 Calle Cañada, San Juan, PR 00920.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

20410325

Citation

Bessoff, Kovi, et al. "Utility of a Commercial Nonstructural Protein 1 Antigen Capture Kit as a Dengue Virus Diagnostic Tool." Clinical and Vaccine Immunology : CVI, vol. 17, no. 6, 2010, pp. 949-53.
Bessoff K, Phoutrides E, Delorey M, et al. Utility of a commercial nonstructural protein 1 antigen capture kit as a dengue virus diagnostic tool. Clin Vaccine Immunol. 2010;17(6):949-53.
Bessoff, K., Phoutrides, E., Delorey, M., Acosta, L. N., & Hunsperger, E. (2010). Utility of a commercial nonstructural protein 1 antigen capture kit as a dengue virus diagnostic tool. Clinical and Vaccine Immunology : CVI, 17(6), 949-53. https://doi.org/10.1128/CVI.00041-10
Bessoff K, et al. Utility of a Commercial Nonstructural Protein 1 Antigen Capture Kit as a Dengue Virus Diagnostic Tool. Clin Vaccine Immunol. 2010;17(6):949-53. PubMed PMID: 20410325.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Utility of a commercial nonstructural protein 1 antigen capture kit as a dengue virus diagnostic tool. AU - Bessoff,Kovi, AU - Phoutrides,Elena, AU - Delorey,Mark, AU - Acosta,Luz N, AU - Hunsperger,Elizabeth, Y1 - 2010/04/21/ PY - 2010/4/23/entrez PY - 2010/4/23/pubmed PY - 2010/12/17/medline SP - 949 EP - 53 JF - Clinical and vaccine immunology : CVI JO - Clin Vaccine Immunol VL - 17 IS - 6 N2 - Annually, over 2.5 billion people are at risk for infection with dengue virus (DENV), while between 50 and 100 million people contract the infection. There is an urgent need for alternative diagnostic tools that can detect DENV during acute infection. Recent studies have shown that DENV nonstructural protein 1 (NS1) is detectable in the blood as early as the onset of symptoms and persists well into the convalescent phase of the infection. We evaluated the utility of the Bio-Rad Platelia DENV NS1 antigen capture kit in combination with real-time reverse transcriptase PCR (RT-PCR) and an IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) for refining a new algorithm for the diagnosis of acute- or convalescent-phase DENV infection with a single clinical sample. We tested the Bio-Rad kit with three panels of sera. These panels were designed to evaluate the sensitivities of the NS1 kit for (i) early-convalescent-phase samples, (ii) acute-phase samples with false-negative PCR results, and (iii) IgM-negative convalescent-phase samples from patients with confirmed secondary DENV infections. Results show that NS1 can be detected in 22% of serum samples collected more than 10 days after the onset of illness and in 22% of samples that did not elicit an IgM response. Additionally, NS1 was detected in 37% of the tested acute-phase samples with false-negative PCR results, suggesting that NS1 detection may be valuable in increasing the sensitivity of current acute-phase diagnostics. These results will improve diagnosis with a single acute-phase or early-convalescent-phase sample for disease surveillance and clinical diagnosis. SN - 1556-679X UR - https://www.unboundmedicine.com/medline/citation/20410325/Utility_of_a_commercial_nonstructural_protein_1_antigen_capture_kit_as_a_dengue_virus_diagnostic_tool_ L2 - http://cvi.asm.org/lookup/pmidlookup?view=long&pmid=20410325 DB - PRIME DP - Unbound Medicine ER -