Tags

Type your tag names separated by a space and hit enter

Analysis of amphetamines and metabolites in urine with ultra performance liquid chromatography tandem mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jun 01; 878(19):1616-22.JC

Abstract

A simple, rapid and sensitive ultra performance liquid chromatography tandem mass spectrometry method was developed and fully validated for the quantitative determination of seven amphetamines and metabolites in urine. The method was validated for selectivity, linearity, LOQ, LOD, imprecision, bias, analyte and processed sample stability, matrix effect, recovery, carryover and dilution integrity. A classic liquid-liquid extraction with ethyl acetate was used as sample preparation procedure. The compounds were separated on an Acquity UPLC HSS C18 column in 6.8 min. The linear dynamic range was established from 25 to 500 ng/mL. The limit of quantification was fixed to the lowest calibrator level and the limit of detection ranged from 0.125 to 2.5 ng/mL. The method presented an excellent intra- and inter-assay imprecision and bias (<10.7%) at each measured concentration of two external quality controls (QC) and three "in house" QC. No matrix effects were observed and good recoveries (>70%) were obtained for all the compounds. No carryover was observed after the analysis of high concentrated samples (8000 ng/mL). The method was subsequently applied to authentic samples.

Authors+Show Affiliations

Federal Public Service Justice, National Institute of Criminalistics and Criminology, Laboratory of Toxicology, Brussels, Belgium. marmixoscity@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20413354

Citation

Ramírez Fernández, María del Mar, et al. "Analysis of Amphetamines and Metabolites in Urine With Ultra Performance Liquid Chromatography Tandem Mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 878, no. 19, 2010, pp. 1616-22.
Ramírez Fernández Mdel M, Wille SM, di Fazio V, et al. Analysis of amphetamines and metabolites in urine with ultra performance liquid chromatography tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2010;878(19):1616-22.
Ramírez Fernández, M. d. e. l. . M., Wille, S. M., di Fazio, V., Gosselin, M., & Samyn, N. (2010). Analysis of amphetamines and metabolites in urine with ultra performance liquid chromatography tandem mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 878(19), 1616-22. https://doi.org/10.1016/j.jchromb.2010.03.048
Ramírez Fernández Mdel M, et al. Analysis of Amphetamines and Metabolites in Urine With Ultra Performance Liquid Chromatography Tandem Mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jun 1;878(19):1616-22. PubMed PMID: 20413354.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of amphetamines and metabolites in urine with ultra performance liquid chromatography tandem mass spectrometry. AU - Ramírez Fernández,María del Mar, AU - Wille,Sarah M R, AU - di Fazio,Vincent, AU - Gosselin,Matthias, AU - Samyn,Nele, Y1 - 2010/04/03/ PY - 2009/12/23/received PY - 2010/03/25/revised PY - 2010/03/26/accepted PY - 2010/4/24/entrez PY - 2010/4/24/pubmed PY - 2010/8/27/medline SP - 1616 EP - 22 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 878 IS - 19 N2 - A simple, rapid and sensitive ultra performance liquid chromatography tandem mass spectrometry method was developed and fully validated for the quantitative determination of seven amphetamines and metabolites in urine. The method was validated for selectivity, linearity, LOQ, LOD, imprecision, bias, analyte and processed sample stability, matrix effect, recovery, carryover and dilution integrity. A classic liquid-liquid extraction with ethyl acetate was used as sample preparation procedure. The compounds were separated on an Acquity UPLC HSS C18 column in 6.8 min. The linear dynamic range was established from 25 to 500 ng/mL. The limit of quantification was fixed to the lowest calibrator level and the limit of detection ranged from 0.125 to 2.5 ng/mL. The method presented an excellent intra- and inter-assay imprecision and bias (<10.7%) at each measured concentration of two external quality controls (QC) and three "in house" QC. No matrix effects were observed and good recoveries (>70%) were obtained for all the compounds. No carryover was observed after the analysis of high concentrated samples (8000 ng/mL). The method was subsequently applied to authentic samples. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/20413354/Analysis_of_amphetamines_and_metabolites_in_urine_with_ultra_performance_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(10)00199-6 DB - PRIME DP - Unbound Medicine ER -