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Heat and exercise acclimation increases intracellular levels of Hsp72 and inhibits exercise-induced increase in intracellular and plasma Hsp72 in humans.
Cell Stress Chaperones. 2010 Nov; 15(6):885-95.CS

Abstract

In order to verify the effects of heat and exercise acclimation (HA) on resting and exercise-induced expression of plasma and leukocyte heat shock protein 72 (Hsp72) in humans, nine healthy young male volunteers (25.0 ± 0.7 years; 80.5 ± 2.0 kg; 180 ± 2 cm, mean ± SE) exercised for 60 min in a hot, dry environment (40 ± 0°C and 45 ± 0% relative humidity) for 11 days. The protocol consisted of running on a treadmill using a controlled hyperthermia technique in which the work rate was adjusted to elevate the rectal temperature by 1°C in 30 min and maintain it elevated for another 30 min. Before and after the HA, the volunteers performed a heat stress test (HST) at 50% of their individual maximal power output for 90 min in the same environment. Blood was drawn before (REST), immediately after (POST) and 1 h after (1 h POST) HST, and plasma and leukocytes were separated and stored. Subjects showed expected adaptations to HA: reduced exercise rectal and mean skin temperatures and heart rate, and augmented sweat rate and exercise tolerance. In HST1, plasma Hsp72 increased from REST to POST and then returned to resting values 1 h POST (REST: 1.11 ± 0.07, POST: 1.48 ± 0.10, 1 h POST: 1.22 ± 0.11 ng mL(-1); p < 0.05). In HST2, there was no change in plasma Hsp72 (REST: 0.94 ± 0.08, POST: 1.20 ± 0.15, 1 h POST: 1.17 ± 0.16 ng mL(-1); p > 0.05). HA increased resting levels of intracellular Hsp72 (HST1: 1 ± 0.02 and HST2: 4.2 ± 1.2 density units, p < 0.05). Exercise-induced increased intracellular Hsp72 expression was observed on HST1 (HST1: REST, 1 ± 0.02 vs. POST, 2.9 ± 0.9 density units, mean ± SE, p < 0.05) but was inhibited on HST2 (HST2: REST, 4.2 ± 1.2 vs. POST, 4.4 ± 1.1 density units, p > 0.05). Regression analysis showed that the lower the pre-exercise expression of intracellular Hsp72, the higher the exercise-induced increase (R = -0.85, p < 0.05). In conclusion, HA increased resting leukocyte Hsp72 levels and inhibited exercise-induced expression. This intracellular adaptation probably induces thermotolerance. In addition, the non-increase in plasma Hsp72 after HA may be related to lower stress at the cellular level in the acclimated individuals.

Authors+Show Affiliations

Laboratory of Biochemistry of Physical Activity, University of São Paulo, São Paulo, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20414820

Citation

Magalhães, Flávio de Castro, et al. "Heat and Exercise Acclimation Increases Intracellular Levels of Hsp72 and Inhibits Exercise-induced Increase in Intracellular and Plasma Hsp72 in Humans." Cell Stress & Chaperones, vol. 15, no. 6, 2010, pp. 885-95.
Magalhães Fde C, Amorim FT, Passos RL, et al. Heat and exercise acclimation increases intracellular levels of Hsp72 and inhibits exercise-induced increase in intracellular and plasma Hsp72 in humans. Cell Stress Chaperones. 2010;15(6):885-95.
Magalhães, F. d. e. . C., Amorim, F. T., Passos, R. L., Fonseca, M. A., Oliveira, K. P., Lima, M. R., Guimarães, J. B., Ferreira-Júnior, J. B., Martini, A. R., Lima, N. R., Soares, D. D., Oliveira, E. M., & Rodrigues, L. O. (2010). Heat and exercise acclimation increases intracellular levels of Hsp72 and inhibits exercise-induced increase in intracellular and plasma Hsp72 in humans. Cell Stress & Chaperones, 15(6), 885-95. https://doi.org/10.1007/s12192-010-0197-7
Magalhães Fde C, et al. Heat and Exercise Acclimation Increases Intracellular Levels of Hsp72 and Inhibits Exercise-induced Increase in Intracellular and Plasma Hsp72 in Humans. Cell Stress Chaperones. 2010;15(6):885-95. PubMed PMID: 20414820.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Heat and exercise acclimation increases intracellular levels of Hsp72 and inhibits exercise-induced increase in intracellular and plasma Hsp72 in humans. AU - Magalhães,Flávio de Castro, AU - Amorim,Fabiano Trigueiro, AU - Passos,Renata L Freitas, AU - Fonseca,Michele Atalla, AU - Oliveira,Kenya Paula Moreira, AU - Lima,Milene Rodrigues Malheiros, AU - Guimarães,Juliana Bohen, AU - Ferreira-Júnior,João Batista, AU - Martini,Angelo R P, AU - Lima,Nilo R V, AU - Soares,Danusa Dias, AU - Oliveira,Edilamar Menezes, AU - Rodrigues,Luiz Oswaldo Carneiro, Y1 - 2010/04/23/ PY - 2008/05/09/received PY - 2010/03/31/accepted PY - 2010/03/26/revised PY - 2010/4/24/entrez PY - 2010/4/24/pubmed PY - 2011/2/5/medline SP - 885 EP - 95 JF - Cell stress & chaperones JO - Cell Stress Chaperones VL - 15 IS - 6 N2 - In order to verify the effects of heat and exercise acclimation (HA) on resting and exercise-induced expression of plasma and leukocyte heat shock protein 72 (Hsp72) in humans, nine healthy young male volunteers (25.0 ± 0.7 years; 80.5 ± 2.0 kg; 180 ± 2 cm, mean ± SE) exercised for 60 min in a hot, dry environment (40 ± 0°C and 45 ± 0% relative humidity) for 11 days. The protocol consisted of running on a treadmill using a controlled hyperthermia technique in which the work rate was adjusted to elevate the rectal temperature by 1°C in 30 min and maintain it elevated for another 30 min. Before and after the HA, the volunteers performed a heat stress test (HST) at 50% of their individual maximal power output for 90 min in the same environment. Blood was drawn before (REST), immediately after (POST) and 1 h after (1 h POST) HST, and plasma and leukocytes were separated and stored. Subjects showed expected adaptations to HA: reduced exercise rectal and mean skin temperatures and heart rate, and augmented sweat rate and exercise tolerance. In HST1, plasma Hsp72 increased from REST to POST and then returned to resting values 1 h POST (REST: 1.11 ± 0.07, POST: 1.48 ± 0.10, 1 h POST: 1.22 ± 0.11 ng mL(-1); p < 0.05). In HST2, there was no change in plasma Hsp72 (REST: 0.94 ± 0.08, POST: 1.20 ± 0.15, 1 h POST: 1.17 ± 0.16 ng mL(-1); p > 0.05). HA increased resting levels of intracellular Hsp72 (HST1: 1 ± 0.02 and HST2: 4.2 ± 1.2 density units, p < 0.05). Exercise-induced increased intracellular Hsp72 expression was observed on HST1 (HST1: REST, 1 ± 0.02 vs. POST, 2.9 ± 0.9 density units, mean ± SE, p < 0.05) but was inhibited on HST2 (HST2: REST, 4.2 ± 1.2 vs. POST, 4.4 ± 1.1 density units, p > 0.05). Regression analysis showed that the lower the pre-exercise expression of intracellular Hsp72, the higher the exercise-induced increase (R = -0.85, p < 0.05). In conclusion, HA increased resting leukocyte Hsp72 levels and inhibited exercise-induced expression. This intracellular adaptation probably induces thermotolerance. In addition, the non-increase in plasma Hsp72 after HA may be related to lower stress at the cellular level in the acclimated individuals. SN - 1466-1268 UR - https://www.unboundmedicine.com/medline/citation/20414820/Heat_and_exercise_acclimation_increases_intracellular_levels_of_Hsp72_and_inhibits_exercise_induced_increase_in_intracellular_and_plasma_Hsp72_in_humans_ L2 - https://dx.doi.org/10.1007/s12192-010-0197-7 DB - PRIME DP - Unbound Medicine ER -