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Diagnosis and phylogenetic analysis of Orf virus from goats in China: a case report.
Virol J 2010; 7:78VJ

Abstract

BACKGROUND

Orf virus (ORFV) is the etiological agent of contagious pustular dermatitis and is the prototype of the genus Parapoxvirus (PPV). It causes a severe exanthematous dermatitis that afflicts domestic and wild small ruminants.

CASE PRESENTATION

In the present study, an outbreak of proliferative dermatitis in farmed goats. The presence of ORFV in tissue scrapings from the lips was confirmed by B2L gene polymerase chain reaction (PCR) amplification. The molecular characterization of the ORFV was performed using PCR amplification, DNA sequencing and phylogenetic analysis of the B2L gene.

CONCLUSION

The results of this investigation indicated that the outbreak was caused by infection with an ORFV that was closely related genetically to Nantou (DQ934351), which was isolated from the Tai wan province of China and Hoping (EU935106), which originated from South Korea in 2008. This is the first report of the phylogenetic analysis of ORFV from goats in China.

Authors+Show Affiliations

Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, Xujiaping No,1, Yanchangpu, Lanzhou, Gansu, 730046, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20416112

Citation

Zhang, Keshan, et al. "Diagnosis and Phylogenetic Analysis of Orf Virus From Goats in China: a Case Report." Virology Journal, vol. 7, 2010, p. 78.
Zhang K, Shang Y, Jin Y, et al. Diagnosis and phylogenetic analysis of Orf virus from goats in China: a case report. Virol J. 2010;7:78.
Zhang, K., Shang, Y., Jin, Y., Wang, G., Zheng, H., He, J., ... Liu, X. (2010). Diagnosis and phylogenetic analysis of Orf virus from goats in China: a case report. Virology Journal, 7, p. 78. doi:10.1186/1743-422X-7-78.
Zhang K, et al. Diagnosis and Phylogenetic Analysis of Orf Virus From Goats in China: a Case Report. Virol J. 2010 Apr 25;7:78. PubMed PMID: 20416112.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnosis and phylogenetic analysis of Orf virus from goats in China: a case report. AU - Zhang,Keshan, AU - Shang,Youjun, AU - Jin,Ye, AU - Wang,Guangxiang, AU - Zheng,Haixue, AU - He,Jijun, AU - Lu,Zhongxin, AU - Liu,Xiangtao, Y1 - 2010/04/25/ PY - 2010/01/20/received PY - 2010/04/25/accepted PY - 2010/4/27/entrez PY - 2010/4/27/pubmed PY - 2010/6/23/medline SP - 78 EP - 78 JF - Virology journal JO - Virol. J. VL - 7 N2 - BACKGROUND: Orf virus (ORFV) is the etiological agent of contagious pustular dermatitis and is the prototype of the genus Parapoxvirus (PPV). It causes a severe exanthematous dermatitis that afflicts domestic and wild small ruminants. CASE PRESENTATION: In the present study, an outbreak of proliferative dermatitis in farmed goats. The presence of ORFV in tissue scrapings from the lips was confirmed by B2L gene polymerase chain reaction (PCR) amplification. The molecular characterization of the ORFV was performed using PCR amplification, DNA sequencing and phylogenetic analysis of the B2L gene. CONCLUSION: The results of this investigation indicated that the outbreak was caused by infection with an ORFV that was closely related genetically to Nantou (DQ934351), which was isolated from the Tai wan province of China and Hoping (EU935106), which originated from South Korea in 2008. This is the first report of the phylogenetic analysis of ORFV from goats in China. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/20416112/Diagnosis_and_phylogenetic_analysis_of_Orf_virus_from_goats_in_China:_a_case_report_ L2 - https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-7-78 DB - PRIME DP - Unbound Medicine ER -