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Development of a high-throughput method for the determination of ethosuximide in human plasma by liquid chromatography mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jun 01; 878(19):1605-10.JC

Abstract

A simple, rapid, sensitive and specific ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantification of ethosuximide in human plasma is described. Analyte was chromatographed on a Hypersil Gold C18 column (100 mm x 2.1 mm, i.d., 1.9 microm) with isocratic elution at a flow rate of 0.250 mL/min and pravastatin was used as the internal standard. The assay involves a simple solid-phase extraction procedure of 0.25 mL human plasma and the analysis was performed on a triple-quadrupole tandem mass spectrometer by MRM mode via electrospray ionization (ESI). The method was linear in the concentration range of 0.25-60.0 microg/mL. The lower limit of quantification (LLOQ) was 0.25 microg/mL. The within- and between-day precision and accuracy of the quality control samples were within 10.0%. The recovery was 95.1% and 94.4% for ethosuximide and pravastatin, respectively. The analysis time for each sample was 1.8 min. The method was highly reproducible and gave peaks with excellent chromatography properties.

Authors+Show Affiliations

Department of Chemistry, Shree U.P. Arts, Smt. M.G. Panchal Science & V.L. Shah Commerce College Pilvai, Vijapur, Gujarat State, India. mitu 070980@yahoo.co.inNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20417157

Citation

Bhatt, Mitesh, et al. "Development of a High-throughput Method for the Determination of Ethosuximide in Human Plasma By Liquid Chromatography Mass Spectrometry." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 878, no. 19, 2010, pp. 1605-10.
Bhatt M, Shah S, Shivprakash . Development of a high-throughput method for the determination of ethosuximide in human plasma by liquid chromatography mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2010;878(19):1605-10.
Bhatt, M., Shah, S., & Shivprakash, . (2010). Development of a high-throughput method for the determination of ethosuximide in human plasma by liquid chromatography mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 878(19), 1605-10. https://doi.org/10.1016/j.jchromb.2010.03.019
Bhatt M, Shah S, Shivprakash . Development of a High-throughput Method for the Determination of Ethosuximide in Human Plasma By Liquid Chromatography Mass Spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jun 1;878(19):1605-10. PubMed PMID: 20417157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a high-throughput method for the determination of ethosuximide in human plasma by liquid chromatography mass spectrometry. AU - Bhatt,Mitesh, AU - Shah,Sanjay, AU - Shivprakash,, Y1 - 2010/03/19/ PY - 2009/11/10/received PY - 2010/03/11/revised PY - 2010/03/11/accepted PY - 2010/4/27/entrez PY - 2010/4/27/pubmed PY - 2010/8/27/medline SP - 1605 EP - 10 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 878 IS - 19 N2 - A simple, rapid, sensitive and specific ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantification of ethosuximide in human plasma is described. Analyte was chromatographed on a Hypersil Gold C18 column (100 mm x 2.1 mm, i.d., 1.9 microm) with isocratic elution at a flow rate of 0.250 mL/min and pravastatin was used as the internal standard. The assay involves a simple solid-phase extraction procedure of 0.25 mL human plasma and the analysis was performed on a triple-quadrupole tandem mass spectrometer by MRM mode via electrospray ionization (ESI). The method was linear in the concentration range of 0.25-60.0 microg/mL. The lower limit of quantification (LLOQ) was 0.25 microg/mL. The within- and between-day precision and accuracy of the quality control samples were within 10.0%. The recovery was 95.1% and 94.4% for ethosuximide and pravastatin, respectively. The analysis time for each sample was 1.8 min. The method was highly reproducible and gave peaks with excellent chromatography properties. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/20417157/Development_of_a_high_throughput_method_for_the_determination_of_ethosuximide_in_human_plasma_by_liquid_chromatography_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(10)00159-5 DB - PRIME DP - Unbound Medicine ER -