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Enumeration of coagulase and thermonuclease-positive Staphylococcus spp. in raw milk and fresh soft cheese: an evaluation of Baird-Parker agar, Rabbit Plasma Fibrinogen agar and the Petrifilm Staph Express count system.
Food Microbiol. 2010 Jun; 27(4):447-52.FM

Abstract

Staphylococcus spp. are microorganisms that are naturally present in milk and dairy products and are often associated with food-borne diseases outbreaks due to the ability of some strains to produce thermostable enterotoxins. This ability is usually associated with coagulase and thermonuclease production, characteristics that are considered in the microbiological analyses for the control of such microorganisms. The objective of this study was to evaluate the culture media and the methodologies used for the enumeration of coagulase and thermonuclease-positive Staphylococcus spp. in raw milk and fresh soft cheese. Samples of artificially contaminated milk (with coagulase-positive Staphylococcus reference strains) and samples of naturally contaminated raw milk and cheese were submitted for enumeration in Baird-Parker agar (BP), Rabbit Plasma Fibrinogen agar (RPFA) and in the Petrifilm Staph Express count system (STX). No significant differences (P > 0.05) were observed between the mean counts obtained in all of the evaluated culture media. RPFA and STX had good correlation indices between the total and typical colony counts as well as with coagulase and the thermonuclease-positive colony counts. Thus, there is a better association between coagulase and thermonuclease production to typical colony morphology developed on these culture media, leading to more accurate and reliable results than with BP, which demonstrated lower correlation indices between these counts.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Viçosa GN
Departamento de Veterinária, Universidade Federal de Viçosa, Campus UFV, 36570 000 Viçosa, MG, Brazil.
Moraes PM
No affiliation info available
Yamazi AK
No affiliation info available
Nero LA
No affiliation info available

MeSH

AgarAnimalsBacteriological TechniquesCheeseCoagulaseColony Count, MicrobialCulture MediaFood ContaminationFood MicrobiologyHumansMicrococcal NucleaseMilkStaphylococcus

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20417392

Citation

Viçosa, Gabriela Nogueira, et al. "Enumeration of Coagulase and Thermonuclease-positive Staphylococcus Spp. in Raw Milk and Fresh Soft Cheese: an Evaluation of Baird-Parker Agar, Rabbit Plasma Fibrinogen Agar and the Petrifilm Staph Express Count System." Food Microbiology, vol. 27, no. 4, 2010, pp. 447-52.
Viçosa GN, Moraes PM, Yamazi AK, et al. Enumeration of coagulase and thermonuclease-positive Staphylococcus spp. in raw milk and fresh soft cheese: an evaluation of Baird-Parker agar, Rabbit Plasma Fibrinogen agar and the Petrifilm Staph Express count system. Food Microbiol. 2010;27(4):447-52.
Viçosa, G. N., Moraes, P. M., Yamazi, A. K., & Nero, L. A. (2010). Enumeration of coagulase and thermonuclease-positive Staphylococcus spp. in raw milk and fresh soft cheese: an evaluation of Baird-Parker agar, Rabbit Plasma Fibrinogen agar and the Petrifilm Staph Express count system. Food Microbiology, 27(4), 447-52. https://doi.org/10.1016/j.fm.2009.12.007
Viçosa GN, et al. Enumeration of Coagulase and Thermonuclease-positive Staphylococcus Spp. in Raw Milk and Fresh Soft Cheese: an Evaluation of Baird-Parker Agar, Rabbit Plasma Fibrinogen Agar and the Petrifilm Staph Express Count System. Food Microbiol. 2010;27(4):447-52. PubMed PMID: 20417392.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enumeration of coagulase and thermonuclease-positive Staphylococcus spp. in raw milk and fresh soft cheese: an evaluation of Baird-Parker agar, Rabbit Plasma Fibrinogen agar and the Petrifilm Staph Express count system. AU - Viçosa,Gabriela Nogueira, AU - Moraes,Paula Mendonça, AU - Yamazi,Anderson Keizo, AU - Nero,Luís Augusto, Y1 - 2010/01/04/ PY - 2009/09/02/received PY - 2009/12/21/revised PY - 2009/12/22/accepted PY - 2010/4/27/entrez PY - 2010/4/27/pubmed PY - 2010/7/16/medline SP - 447 EP - 52 JF - Food microbiology JO - Food Microbiol VL - 27 IS - 4 N2 - Staphylococcus spp. are microorganisms that are naturally present in milk and dairy products and are often associated with food-borne diseases outbreaks due to the ability of some strains to produce thermostable enterotoxins. This ability is usually associated with coagulase and thermonuclease production, characteristics that are considered in the microbiological analyses for the control of such microorganisms. The objective of this study was to evaluate the culture media and the methodologies used for the enumeration of coagulase and thermonuclease-positive Staphylococcus spp. in raw milk and fresh soft cheese. Samples of artificially contaminated milk (with coagulase-positive Staphylococcus reference strains) and samples of naturally contaminated raw milk and cheese were submitted for enumeration in Baird-Parker agar (BP), Rabbit Plasma Fibrinogen agar (RPFA) and in the Petrifilm Staph Express count system (STX). No significant differences (P > 0.05) were observed between the mean counts obtained in all of the evaluated culture media. RPFA and STX had good correlation indices between the total and typical colony counts as well as with coagulase and the thermonuclease-positive colony counts. Thus, there is a better association between coagulase and thermonuclease production to typical colony morphology developed on these culture media, leading to more accurate and reliable results than with BP, which demonstrated lower correlation indices between these counts. SN - 1095-9998 UR - https://www.unboundmedicine.com/medline/citation/20417392/Enumeration_of_coagulase_and_thermonuclease_positive_Staphylococcus_spp__in_raw_milk_and_fresh_soft_cheese:_an_evaluation_of_Baird_Parker_agar_Rabbit_Plasma_Fibrinogen_agar_and_the_Petrifilm_Staph_Express_count_system_ DB - PRIME DP - Unbound Medicine ER -