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Inactivation kinetics of pulsed electric field-resistant strains of Listeria monocytogenes and Staphylococcus aureus in media of different pH.
Food Microbiol. 2010 Jun; 27(4):550-8.FM

Abstract

A study of the effect of pulsed electric fields (PEF) on the inactivation of Listeria monocytogenes STCC 5672 and Staphylococcus aureus STCC 4459 in McIlvaine buffer covering a range from pH 3.5 to 7.0 was conducted. Mathematical models based on the Weibull distribution were developed to describe the influence of the electric field strength, treatment time and pH of the treatment medium on the lethality of both Gram positive pathogenic bacteria after PEF treatments. Both microorganisms were more sensitive to PEF in media of low pH, although the influence of the pH on the PEF resistance was more significant in S. aureus. In the best cases scenario, the highest inactivation levels achieved were 3.3 and 6.1 log(10) cycles for L. monocytogenes and S. aureus respectively in pH 3.5 after 500 micros of 35 kV/cm. Based on these results and those observed in literature, L. monocytogenes STCC 5672 at any pH investigated has been shown as one of the most PEF resistant microorganism. Therefore, this microorganism should be considered as a possible target microorganism to define process criterion for PEF pasteurization.

Authors+Show Affiliations

Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50.013 Zaragoza, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20417406

Citation

Saldaña, G, et al. "Inactivation Kinetics of Pulsed Electric Field-resistant Strains of Listeria Monocytogenes and Staphylococcus Aureus in Media of Different PH." Food Microbiology, vol. 27, no. 4, 2010, pp. 550-8.
Saldaña G, Puértolas E, Condón S, et al. Inactivation kinetics of pulsed electric field-resistant strains of Listeria monocytogenes and Staphylococcus aureus in media of different pH. Food Microbiol. 2010;27(4):550-8.
Saldaña, G., Puértolas, E., Condón, S., Alvarez, I., & Raso, J. (2010). Inactivation kinetics of pulsed electric field-resistant strains of Listeria monocytogenes and Staphylococcus aureus in media of different pH. Food Microbiology, 27(4), 550-8. https://doi.org/10.1016/j.fm.2010.01.002
Saldaña G, et al. Inactivation Kinetics of Pulsed Electric Field-resistant Strains of Listeria Monocytogenes and Staphylococcus Aureus in Media of Different PH. Food Microbiol. 2010;27(4):550-8. PubMed PMID: 20417406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inactivation kinetics of pulsed electric field-resistant strains of Listeria monocytogenes and Staphylococcus aureus in media of different pH. AU - Saldaña,G, AU - Puértolas,E, AU - Condón,S, AU - Alvarez,I, AU - Raso,J, Y1 - 2010/01/25/ PY - 2009/06/15/received PY - 2010/01/14/revised PY - 2010/01/17/accepted PY - 2010/4/27/entrez PY - 2010/4/27/pubmed PY - 2010/7/16/medline SP - 550 EP - 8 JF - Food microbiology JO - Food Microbiol VL - 27 IS - 4 N2 - A study of the effect of pulsed electric fields (PEF) on the inactivation of Listeria monocytogenes STCC 5672 and Staphylococcus aureus STCC 4459 in McIlvaine buffer covering a range from pH 3.5 to 7.0 was conducted. Mathematical models based on the Weibull distribution were developed to describe the influence of the electric field strength, treatment time and pH of the treatment medium on the lethality of both Gram positive pathogenic bacteria after PEF treatments. Both microorganisms were more sensitive to PEF in media of low pH, although the influence of the pH on the PEF resistance was more significant in S. aureus. In the best cases scenario, the highest inactivation levels achieved were 3.3 and 6.1 log(10) cycles for L. monocytogenes and S. aureus respectively in pH 3.5 after 500 micros of 35 kV/cm. Based on these results and those observed in literature, L. monocytogenes STCC 5672 at any pH investigated has been shown as one of the most PEF resistant microorganism. Therefore, this microorganism should be considered as a possible target microorganism to define process criterion for PEF pasteurization. SN - 1095-9998 UR - https://www.unboundmedicine.com/medline/citation/20417406/Inactivation_kinetics_of_pulsed_electric_field_resistant_strains_of_Listeria_monocytogenes_and_Staphylococcus_aureus_in_media_of_different_pH_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0740-0020(10)00016-X DB - PRIME DP - Unbound Medicine ER -