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Phenolic compounds in different barley varieties: identification by tandem mass spectrometry (QStar) and NMR; quantification by liquid chromatography triple quadrupole-linear ion trap mass spectrometry (Q-Trap).
Nat Prod Commun. 2010 Mar; 5(3):407-14.NP

Abstract

Barley (Hordeum vulgare) is an important cereal that has many applications; as a human food, in malt products and as livestock feed. The content of soluble health-promoting fibers, beta-glucans, varies substantially among barley varieties. In the present study, the content of secondary metabolites with potential positive health effects in different high- and low-beta-glucan barley varieties was studied. Five different flavanols were isolated and identified: (2R,3S)-catechin-7-O-beta-D-glucopyranoside (1), prodelphinidin B3 (2), procyanidin B3 (3), (+)-catechin (4) and procyanidin B1 (5). Procyanidin B1 has never been reported in barley grains before. The compounds were identified using 1H NMR and quadrupolar time-of-flight mass spectrometry. A quantitative analytical method was developed for prodelphinidin B3, procyanidin B3 and (+)-catechin in liquid chromatography triple quadrupole-linear ion trap mass spectrometry and these compounds were quantified in all varieties, together with four phenolic acids: ferulic acid, vanillic acid, p-coumaric acid and p-hydroxybenzoic acid. Catechin was the compound that was present at the highest concentration in all varieties. The variation, between cultivars, in catechin concentration varied four fold. A Principal Component Analysis indicated no correlation between concentrations of beta-glucan and secondary metabolites. Concentrations of catechin and prodelphinidin B3 were strongly correlated, whereas the concentration of procyanidin B3 was not correlated with that of catechin or prodelphinidin B3. Either two different enzymes could be responsible for the dimerization of prodelphinidin B3 and procyanidin B3, or the affinity of the enzyme could be different whether the dimerization is between two catechin units or between units of gallocatechin and catechin.

Authors+Show Affiliations

University of Aarhus, Faculty of Agricultural Sciences, Department of Integrated Pest Management, DK-4200 Slagelse, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20420318

Citation

Klausen, Kamilla, et al. "Phenolic Compounds in Different Barley Varieties: Identification By Tandem Mass Spectrometry (QStar) and NMR; Quantification By Liquid Chromatography Triple Quadrupole-linear Ion Trap Mass Spectrometry (Q-Trap)." Natural Product Communications, vol. 5, no. 3, 2010, pp. 407-14.
Klausen K, Mortensen AG, Laursen B, et al. Phenolic compounds in different barley varieties: identification by tandem mass spectrometry (QStar) and NMR; quantification by liquid chromatography triple quadrupole-linear ion trap mass spectrometry (Q-Trap). Nat Prod Commun. 2010;5(3):407-14.
Klausen, K., Mortensen, A. G., Laursen, B., Haselmann, K. F., Jespersen, B. M., & Fomsgaard, I. S. (2010). Phenolic compounds in different barley varieties: identification by tandem mass spectrometry (QStar) and NMR; quantification by liquid chromatography triple quadrupole-linear ion trap mass spectrometry (Q-Trap). Natural Product Communications, 5(3), 407-14.
Klausen K, et al. Phenolic Compounds in Different Barley Varieties: Identification By Tandem Mass Spectrometry (QStar) and NMR; Quantification By Liquid Chromatography Triple Quadrupole-linear Ion Trap Mass Spectrometry (Q-Trap). Nat Prod Commun. 2010;5(3):407-14. PubMed PMID: 20420318.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenolic compounds in different barley varieties: identification by tandem mass spectrometry (QStar) and NMR; quantification by liquid chromatography triple quadrupole-linear ion trap mass spectrometry (Q-Trap). AU - Klausen,Kamilla, AU - Mortensen,Anne G, AU - Laursen,Bente, AU - Haselmann,Kim F, AU - Jespersen,Birthe Møller, AU - Fomsgaard,Inge S, PY - 2010/4/28/entrez PY - 2010/4/28/pubmed PY - 2010/5/21/medline SP - 407 EP - 14 JF - Natural product communications JO - Nat Prod Commun VL - 5 IS - 3 N2 - Barley (Hordeum vulgare) is an important cereal that has many applications; as a human food, in malt products and as livestock feed. The content of soluble health-promoting fibers, beta-glucans, varies substantially among barley varieties. In the present study, the content of secondary metabolites with potential positive health effects in different high- and low-beta-glucan barley varieties was studied. Five different flavanols were isolated and identified: (2R,3S)-catechin-7-O-beta-D-glucopyranoside (1), prodelphinidin B3 (2), procyanidin B3 (3), (+)-catechin (4) and procyanidin B1 (5). Procyanidin B1 has never been reported in barley grains before. The compounds were identified using 1H NMR and quadrupolar time-of-flight mass spectrometry. A quantitative analytical method was developed for prodelphinidin B3, procyanidin B3 and (+)-catechin in liquid chromatography triple quadrupole-linear ion trap mass spectrometry and these compounds were quantified in all varieties, together with four phenolic acids: ferulic acid, vanillic acid, p-coumaric acid and p-hydroxybenzoic acid. Catechin was the compound that was present at the highest concentration in all varieties. The variation, between cultivars, in catechin concentration varied four fold. A Principal Component Analysis indicated no correlation between concentrations of beta-glucan and secondary metabolites. Concentrations of catechin and prodelphinidin B3 were strongly correlated, whereas the concentration of procyanidin B3 was not correlated with that of catechin or prodelphinidin B3. Either two different enzymes could be responsible for the dimerization of prodelphinidin B3 and procyanidin B3, or the affinity of the enzyme could be different whether the dimerization is between two catechin units or between units of gallocatechin and catechin. SN - 1934-578X UR - https://www.unboundmedicine.com/medline/citation/20420318/Phenolic_compounds_in_different_barley_varieties:_identification_by_tandem_mass_spectrometry__QStar__and_NMR DB - PRIME DP - Unbound Medicine ER -