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An LC-ESI/MS method for determining theanine in green tea dietary supplements.
Anal Bioanal Chem. 2010 Jul; 397(5):1773-7.AB

Abstract

Theanine is the major amino acid present in Camellia sinensis or green tea. A method for determining theanine in its native state using liquid chromatography with positive-mode electrospray ionization mass spectrometric detection was developed. Quantitation of theanine was achieved using theanine-[(2)H(5)] as an internal standard. This approach was utilized on different green tea matrix materials that are commonly used as dietary supplements including powdered plant leaves, a powdered plant leaf extract, and an oral dosage form that contains green tea. The theanine response was linear over several orders of magnitude, and excellent measurement precision was obtained for all three materials using the developed method.

Authors+Show Affiliations

Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20899-8392, USA. mary.bedner@nist.govNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

20422160

Citation

Bedner, Mary, et al. "An LC-ESI/MS Method for Determining Theanine in Green Tea Dietary Supplements." Analytical and Bioanalytical Chemistry, vol. 397, no. 5, 2010, pp. 1773-7.
Bedner M, Sander LC, Sharpless KE. An LC-ESI/MS method for determining theanine in green tea dietary supplements. Anal Bioanal Chem. 2010;397(5):1773-7.
Bedner, M., Sander, L. C., & Sharpless, K. E. (2010). An LC-ESI/MS method for determining theanine in green tea dietary supplements. Analytical and Bioanalytical Chemistry, 397(5), 1773-7. https://doi.org/10.1007/s00216-010-3713-9
Bedner M, Sander LC, Sharpless KE. An LC-ESI/MS Method for Determining Theanine in Green Tea Dietary Supplements. Anal Bioanal Chem. 2010;397(5):1773-7. PubMed PMID: 20422160.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An LC-ESI/MS method for determining theanine in green tea dietary supplements. AU - Bedner,Mary, AU - Sander,Lane C, AU - Sharpless,Katherine E, Y1 - 2010/04/28/ PY - 2010/02/02/received PY - 2010/03/31/accepted PY - 2010/03/26/revised PY - 2010/4/28/entrez PY - 2010/4/28/pubmed PY - 2010/9/21/medline SP - 1773 EP - 7 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 397 IS - 5 N2 - Theanine is the major amino acid present in Camellia sinensis or green tea. A method for determining theanine in its native state using liquid chromatography with positive-mode electrospray ionization mass spectrometric detection was developed. Quantitation of theanine was achieved using theanine-[(2)H(5)] as an internal standard. This approach was utilized on different green tea matrix materials that are commonly used as dietary supplements including powdered plant leaves, a powdered plant leaf extract, and an oral dosage form that contains green tea. The theanine response was linear over several orders of magnitude, and excellent measurement precision was obtained for all three materials using the developed method. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/20422160/An_LC_ESI/MS_method_for_determining_theanine_in_green_tea_dietary_supplements_ L2 - https://dx.doi.org/10.1007/s00216-010-3713-9 DB - PRIME DP - Unbound Medicine ER -