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Calgranulin B (S100A9/MRP14): a key molecule in idiopathic pulmonary fibrosis?
Inflammation. 2011 Apr; 34(2):85-91.I

Abstract

Calgranulin B is a small calcium-binding protein with several immunological functions mainly involved in chronic inflammation and cancer. It can participate in recruitment of neutrophils and leukocytes in inflamed tissue, oxidant/antioxidant balance, adhesion of neutrophils to fibronectin, and regulation of apoptosis. In a previous proteomic study, we found that calgranulin B was up-regulated in the bronchoalveolar lavage (BAL) of patients with idiopathic pulmonary fibrosis (IPF) with respect to controls and patients with other interstitial lung diseases. The aims of this study are to compare calgranulin B concentrations in BAL of patients with IPF and sarcoidosis and controls by a quantitative method, to look for correlations with clinical data, and to evaluate calgranulin B expression in lung tissue of IPF patients by immunohistochemistry. A modification of a commercial ELISA was used to determine calgranulin B concentrations in BAL of 16 patients with IPF (a group of patients in which we previously performed proteomic analysis), 17 patients with sarcoidosis, and 7 controls. The immunohistochemistry was done in a subgroup of patients with IPF and a control group of lung transplant donors. Calgranulin B concentrations were significantly higher in patients with IPF than controls (p < 0.01); they were inversely correlated with FVC and DLCO values and directly correlated with neutrophil and eosinophil percentages in BAL. Immunohistochemistry revealed a patchy distribution of calgranulin B, predominantly around areas of fibrotic remodeling. Calgranulin B may be a trigger molecule involved in the evolution and progression of IPF, being overexpressed in BAL of patients with IPF with severe functional deterioration and in the peribronchiolar area bordering zones of honeycombing.

Authors+Show Affiliations

Respiratory Diseases Section, Dept. of Clinical Medicine and Immunological Sciences, Siena University, Siena, Italy. bargagli2@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20422274

Citation

Bargagli, Elena, et al. "Calgranulin B (S100A9/MRP14): a Key Molecule in Idiopathic Pulmonary Fibrosis?" Inflammation, vol. 34, no. 2, 2011, pp. 85-91.
Bargagli E, Olivieri C, Cintorino M, et al. Calgranulin B (S100A9/MRP14): a key molecule in idiopathic pulmonary fibrosis? Inflammation. 2011;34(2):85-91.
Bargagli, E., Olivieri, C., Cintorino, M., Refini, R. M., Bianchi, N., Prasse, A., & Rottoli, P. (2011). Calgranulin B (S100A9/MRP14): a key molecule in idiopathic pulmonary fibrosis? Inflammation, 34(2), 85-91. https://doi.org/10.1007/s10753-010-9210-7
Bargagli E, et al. Calgranulin B (S100A9/MRP14): a Key Molecule in Idiopathic Pulmonary Fibrosis. Inflammation. 2011;34(2):85-91. PubMed PMID: 20422274.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Calgranulin B (S100A9/MRP14): a key molecule in idiopathic pulmonary fibrosis? AU - Bargagli,Elena, AU - Olivieri,Carmela, AU - Cintorino,Marcella, AU - Refini,Rosa M, AU - Bianchi,Nicola, AU - Prasse,Antje, AU - Rottoli,Paola, PY - 2010/4/28/entrez PY - 2010/4/28/pubmed PY - 2011/6/17/medline SP - 85 EP - 91 JF - Inflammation JO - Inflammation VL - 34 IS - 2 N2 - Calgranulin B is a small calcium-binding protein with several immunological functions mainly involved in chronic inflammation and cancer. It can participate in recruitment of neutrophils and leukocytes in inflamed tissue, oxidant/antioxidant balance, adhesion of neutrophils to fibronectin, and regulation of apoptosis. In a previous proteomic study, we found that calgranulin B was up-regulated in the bronchoalveolar lavage (BAL) of patients with idiopathic pulmonary fibrosis (IPF) with respect to controls and patients with other interstitial lung diseases. The aims of this study are to compare calgranulin B concentrations in BAL of patients with IPF and sarcoidosis and controls by a quantitative method, to look for correlations with clinical data, and to evaluate calgranulin B expression in lung tissue of IPF patients by immunohistochemistry. A modification of a commercial ELISA was used to determine calgranulin B concentrations in BAL of 16 patients with IPF (a group of patients in which we previously performed proteomic analysis), 17 patients with sarcoidosis, and 7 controls. The immunohistochemistry was done in a subgroup of patients with IPF and a control group of lung transplant donors. Calgranulin B concentrations were significantly higher in patients with IPF than controls (p < 0.01); they were inversely correlated with FVC and DLCO values and directly correlated with neutrophil and eosinophil percentages in BAL. Immunohistochemistry revealed a patchy distribution of calgranulin B, predominantly around areas of fibrotic remodeling. Calgranulin B may be a trigger molecule involved in the evolution and progression of IPF, being overexpressed in BAL of patients with IPF with severe functional deterioration and in the peribronchiolar area bordering zones of honeycombing. SN - 1573-2576 UR - https://www.unboundmedicine.com/medline/citation/20422274/Calgranulin_B__S100A9/MRP14_:_a_key_molecule_in_idiopathic_pulmonary_fibrosis L2 - https://doi.org/10.1007/s10753-010-9210-7 DB - PRIME DP - Unbound Medicine ER -