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A mutation in the cytosolic O-acetylserine (thiol) lyase induces a genome-dependent early leaf death phenotype in Arabidopsis.
BMC Plant Biol 2010; 10:80BP

Abstract

BACKGROUND

Cysteine is a component in organic compounds including glutathione that have been implicated in the adaptation of plants to stresses. O-acetylserine (thiol) lyase (OAS-TL) catalyses the final step of cysteine biosynthesis. OAS-TL enzyme isoforms are localised in the cytoplasm, the plastids and mitochondria but the contribution of individual OAS-TL isoforms to plant sulphur metabolism has not yet been fully clarified.

RESULTS

The seedling lethal phenotype of the Arabidopsis onset of leaf death3-1 (old3-1) mutant is due to a point mutation in the OAS-A1 gene, encoding the cytosolic OAS-TL. The mutation causes a single amino acid substitution from Gly162 to Glu162, abolishing old3-1 OAS-TL activity in vitro. The old3-1 mutation segregates as a monogenic semi-dominant trait when backcrossed to its wild type accession Landsberg erecta (Ler-0) and the Di-2 accession. Consistent with its semi-dominant behaviour, wild type Ler-0 plants transformed with the mutated old3-1 gene, displayed the early leaf death phenotype. However, the old3-1 mutation segregates in an 11:4:1 (wild type: semi-dominant: mutant) ratio when backcrossed to the Colombia-0 and Wassilewskija accessions. Thus, the early leaf death phenotype depends on two semi-dominant loci. The second locus that determines the old3-1 early leaf death phenotype is referred to as odd-ler (for old3 determinant in the Ler accession) and is located on chromosome 3. The early leaf death phenotype is temperature dependent and is associated with increased expression of defence-response and oxidative-stress marker genes. Independent of the presence of the odd-ler gene, OAS-A1 is involved in maintaining sulphur and thiol levels and is required for resistance against cadmium stress.

CONCLUSIONS

The cytosolic OAS-TL is involved in maintaining organic sulphur levels. The old3-1 mutation causes genome-dependent and independent phenotypes and uncovers a novel function for the mutated OAS-TL in cell death regulation.

Authors+Show Affiliations

Molecular Biology of Plants, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, Haren, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20429919

Citation

Shirzadian-Khorramabad, Reza, et al. "A Mutation in the Cytosolic O-acetylserine (thiol) Lyase Induces a Genome-dependent Early Leaf Death Phenotype in Arabidopsis." BMC Plant Biology, vol. 10, 2010, p. 80.
Shirzadian-Khorramabad R, Jing HC, Everts GE, et al. A mutation in the cytosolic O-acetylserine (thiol) lyase induces a genome-dependent early leaf death phenotype in Arabidopsis. BMC Plant Biol. 2010;10:80.
Shirzadian-Khorramabad, R., Jing, H. C., Everts, G. E., Schippers, J. H., Hille, J., & Dijkwel, P. P. (2010). A mutation in the cytosolic O-acetylserine (thiol) lyase induces a genome-dependent early leaf death phenotype in Arabidopsis. BMC Plant Biology, 10, p. 80. doi:10.1186/1471-2229-10-80.
Shirzadian-Khorramabad R, et al. A Mutation in the Cytosolic O-acetylserine (thiol) Lyase Induces a Genome-dependent Early Leaf Death Phenotype in Arabidopsis. BMC Plant Biol. 2010 Apr 29;10:80. PubMed PMID: 20429919.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A mutation in the cytosolic O-acetylserine (thiol) lyase induces a genome-dependent early leaf death phenotype in Arabidopsis. AU - Shirzadian-Khorramabad,Reza, AU - Jing,Hai-Chun, AU - Everts,Gerja E, AU - Schippers,Jos H M, AU - Hille,Jacques, AU - Dijkwel,Paul P, Y1 - 2010/04/29/ PY - 2009/09/08/received PY - 2010/04/29/accepted PY - 2010/5/1/entrez PY - 2010/5/1/pubmed PY - 2010/7/6/medline SP - 80 EP - 80 JF - BMC plant biology JO - BMC Plant Biol. VL - 10 N2 - BACKGROUND: Cysteine is a component in organic compounds including glutathione that have been implicated in the adaptation of plants to stresses. O-acetylserine (thiol) lyase (OAS-TL) catalyses the final step of cysteine biosynthesis. OAS-TL enzyme isoforms are localised in the cytoplasm, the plastids and mitochondria but the contribution of individual OAS-TL isoforms to plant sulphur metabolism has not yet been fully clarified. RESULTS: The seedling lethal phenotype of the Arabidopsis onset of leaf death3-1 (old3-1) mutant is due to a point mutation in the OAS-A1 gene, encoding the cytosolic OAS-TL. The mutation causes a single amino acid substitution from Gly162 to Glu162, abolishing old3-1 OAS-TL activity in vitro. The old3-1 mutation segregates as a monogenic semi-dominant trait when backcrossed to its wild type accession Landsberg erecta (Ler-0) and the Di-2 accession. Consistent with its semi-dominant behaviour, wild type Ler-0 plants transformed with the mutated old3-1 gene, displayed the early leaf death phenotype. However, the old3-1 mutation segregates in an 11:4:1 (wild type: semi-dominant: mutant) ratio when backcrossed to the Colombia-0 and Wassilewskija accessions. Thus, the early leaf death phenotype depends on two semi-dominant loci. The second locus that determines the old3-1 early leaf death phenotype is referred to as odd-ler (for old3 determinant in the Ler accession) and is located on chromosome 3. The early leaf death phenotype is temperature dependent and is associated with increased expression of defence-response and oxidative-stress marker genes. Independent of the presence of the odd-ler gene, OAS-A1 is involved in maintaining sulphur and thiol levels and is required for resistance against cadmium stress. CONCLUSIONS: The cytosolic OAS-TL is involved in maintaining organic sulphur levels. The old3-1 mutation causes genome-dependent and independent phenotypes and uncovers a novel function for the mutated OAS-TL in cell death regulation. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/20429919/A_mutation_in_the_cytosolic_O_acetylserine__thiol__lyase_induces_a_genome_dependent_early_leaf_death_phenotype_in_Arabidopsis_ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-10-80 DB - PRIME DP - Unbound Medicine ER -